We narrowed to 19,454 results for: gateway
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Plasmid#98608PurposeMammalian DamID lentiviral vector for fusing ELK1Y158A with Dam-V5 using Gateway cloningDepositorInsertELK1Y158A (Elk1 Mouse)
UseLentiviral; DamidTagsDam (DNA adenine methyltransferase) and V5ExpressionMammalianPromoterHeat Shock Minimal PromoterAvailable sinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENTR CMVie-Lifeact-mCardinal L1-R5
Plasmid#84392PurposeMultisite Gateway entry vector for expression of mCardinal-tagged Lifeact in cells including neuronsDepositorInsertLifeact
UseGateway CloningTagsmCardinalExpressionMammalianPromoterCMV immediate earlyAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
8522-E10
Plasmid#234295PurposeGateway ORF Entry clone of human SMAD2 with stop codon (for native or N-terminal fusions); S464A/S465A/S467A mutations (C-terminal phosphorylation sites dead)DepositorInsertSMAD2 S464A/S465A/S467A (SMAD2 Human)
UseGateway CloningMutationS464A/S465A/S467A; C-terminal phosphorylation sit…Available sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
9336-E23
Plasmid#234306PurposeGateway ORF Entry clone of human SMAD3 EPSM-CSD with stop codon (for native or N-terminal fusions); Linker sites dead, C-terminal constitutively activeDepositorInsertSMAD3 EPSM-CSD (SMAD3 Human)
UseGateway CloningMutationT179V/S204A/S208A/S213A/S423D/S425D; Linker sites…Available sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4b G12D/C185S
Plasmid#83165PurposeGateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12D/C185S mutationDepositorAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pREP3xN_ccdB
Plasmid#79021PurposeGateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1 (ATATATAAA) promoter, LEU2 selectionDepositorTypeEmpty backboneExpressionYeastAvailable sinceJuly 7, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAM88
Plasmid#226680PurposeRpt1I191TAG base (3xFLAG-Rpt1I191TAG, Rpt2, 6xHis-Rpt3, Rpt4, Rpt5, Rpt6)DepositorInsertsTagsFLAG and HisExpressionBacterialMutationI191TAGAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM89
Plasmid#226681PurposeRpt5Q49TAG base (3xFLAG-Rpt1, Rpt2, 6xHis-Rpt3, Rpt4, Rpt5Q49TAG, Rpt6)DepositorInsertsTagsFLAG and HisExpressionBacterialMutationQ49TAGAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe3-RfA-HA
Plasmid#186374PurposeDestination vector for the expression of a fusion of an N-ter protein of interest with an HA tag into embryos, by mRNA microinjectionDepositorPublicationTypeEmpty backboneUseGateway CloningAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe3-HA-RfA
Plasmid#186373PurposeDestination vector for the expression of a fusion of N-ter HA tag and a protein of interest into embryos, by mRNA microinjectionDepositorPublicationTypeEmpty backboneUseGateway CloningAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe3-Venus-RfA
Plasmid#186371PurposeDestination vector for the expression of a fluorescent fusion of N-ter Venus and a protein of interest into embryos, by mRNA microinjectionDepositorPublicationTypeEmpty backboneUseGateway CloningAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnj20
Plasmid#173150PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj20 (si:ch211-113j13.2 Zebrafish)
UsePcr cloning vectorPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnj12a
Plasmid#173141PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj12a (kcnj12a Zebrafish)
UsePcr cloning vectorPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnj12b
Plasmid#173142PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj12b (kcnj12b Zebrafish)
UsePcr cloning vectorPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-kcnj13
Plasmid#173143PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj13 (kcnj13 Zebrafish)
UsePcr cloning vectorPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-kcnj15
Plasmid#173145PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj15 (kcnj15 Zebrafish)
UsePcr cloning vectorPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-kcnj16
Plasmid#173146PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj16a (kcnj16 Zebrafish)
UsePcr cloning vectorMutationStop codon removedPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnj19a
Plasmid#173148PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj19a (kcnj19a Zebrafish)
UsePcr cloning vectorPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnj19b
Plasmid#173149PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj19b (kcnj19b Zebrafish)
UsePcr cloning vectorPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnj21
Plasmid#173151PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj21 (zgc:162160 Zebrafish)
UsePcr cloning vectorPromoterNo promoter at 5 end; T7 promoter at 3 endAvailable sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only