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pET-28b His6-TEV-SHP2-FL(E139A)

Plasmid

#245207

Purpose

Production of His-tagged full-length SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Jul 4:2025.07.0

Insert

SHP2 full-length

Tags

6xHis

Expression

Bacterial

Mutation

E139A

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28b His6-TEV-SHP2-FL(E139D)

Plasmid

#245208

Purpose

Production of His-tagged full-length SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al Proc Natl Acad Sci U S A. 2024 Jul 23;12

Insert

SHP2 full-length

Tags

6xHis

Expression

Bacterial

Mutation

E139D

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28b His6-TEV-SHP2-FL(I282V)

Plasmid

#245210

Purpose

Production of His-tagged full-length SHP2

Depositor

Neel Shah

Article

Jiang et al Nat Commun. 2025 Jul 1;16(1):5464. doi:

Insert

SHP2 full-length

Tags

6xHis

Expression

Bacterial

Mutation

I282V

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28b His6-TEV-SHP2-FL(A72V)

Plasmid

#245200

Purpose

Production of His-tagged full-length SHP2

Depositor

Neel Shah

Article

Jiang et al Nat Commun. 2025 Jul 1;16(1):5464. doi:

Insert

SHP2 full-length

Tags

6xHis

Expression

Bacterial

Mutation

A72V

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28b His6-TEV-SHP2-FL(T356L)

Plasmid

#245211

Purpose

Production of His-tagged full-length SHP2

Depositor

Neel Shah

Article

Jiang et al Nat Commun. 2025 Jul 1;16(1):5464. doi:

Insert

SHP2 full-length

Tags

6xHis

Expression

Bacterial

Mutation

T356L

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28b His6-TEV-SHP2-FL(P424L)

Plasmid

#245212

Purpose

Production of His-tagged full-length SHP2

Depositor

Neel Shah

Article

Jiang et al Nat Commun. 2025 Jul 1;16(1):5464. doi:

Insert

SHP2 full-length

Tags

6xHis

Expression

Bacterial

Mutation

P424L

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28b His6-TEV-SHP2-FL(D61Y)

Plasmid

#245198

Purpose

Production of His-tagged full-length SHP2

Depositor

Neel Shah

Article

Jiang et al Nat Commun. 2025 Jul 1;16(1):5464. doi:

Insert

SHP2 full-length

Tags

6xHis

Expression

Bacterial

Mutation

D61Y

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28b His6-TEV-SHP2-FL(H114W)

Plasmid

#245205

Purpose

Production of His-tagged full-length SHP2

Depositor

Neel Shah

Article

Jiang et al Nat Commun. 2025 Jul 1;16(1):5464. doi:

Insert

SHP2 full-length

Tags

6xHis

Expression

Bacterial

Mutation

H114W

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28b His6-TEV-SHP2-FL(E69K)

Plasmid

#245199

Purpose

Production of His-tagged full-length SHP2

Depositor

Neel Shah

Article

Jiang et al Nat Commun. 2025 Jul 1;16(1):5464. doi:

Insert

SHP2 full-length

Tags

6xHis

Expression

Bacterial

Mutation

E69K

Available Since

Sept. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pET-SUMO-SHP2-C-SH2(E139D)-Avi

Plasmid

#245190

Purpose

Production of N-terminally His-SUMO-tagged and C-terminally Avi-tagged SH2 domain

Depositor

Neel Shah

Article

van Vlimmeren et al Proc Natl Acad Sci U S A. 2024 Jul 23;12

Insert

SHP2 C-SH2

Tags

Avi and His-SUMO

Expression

Bacterial

Mutation

E139D

Available Since

Sept. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pET15b His SUMO mNeon L66R PPM1H

Plasmid

#240064

Purpose

Expresses His SUMO mNeon (inserted in loop 1) L66R PPM1H phosphtase in bacterial cells

Depositor

Suzanne Pfeffer

Article

Adhikari et al J Biol Chem. 2025 Sep 3:110679. doi: 10.

Insert

His SUMO mNeon (loop 1) L66R PPM1H (PPM1H Human)

Expression

Bacterial

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pET15b His SUMO mNeon Δ37 PPM1H

Plasmid

#240065

Purpose

Expresses His SUMO mNeon (inserted in loop1) Δ37 PPM1H in bacterial cells

Depositor

Suzanne Pfeffer

Article

Adhikari et al J Biol Chem. 2025 Sep 3:110679. doi: 10.

Insert

His SUMO mNeon (loop 1) Δ37 PPM1H (PPM1H Human)

Expression

Bacterial

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pSIN-FHA2 R605A-Ypet-34-ECFP-hLaminA

Plasmid

#235123

Purpose

FHA2-mutated FRET biosensor R605A; FRET biosensor for live cell visualization of pSer22 Lamin A

Depositor

Qin Peng

Article

Liu et al Biomater Res. 2024 Oct 22;28:0091. doi:

Insert

FHA2 R605A-Ypet-34-ECFP-hLaminA

Use

Lentiviral

Expression

Mammalian

Mutation

FHA2 R605A

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR2b ITGA2-tail-YPet (GFFKR/GAAKR)

Plasmid

#145144

Purpose

Shuttle vector with YPet-tagged ITGA2 cytoplasmic tail as an insert with a GFFKR/GAAKR mutation

Depositor

Johanna Ivaska

Article

Miihkinen et al Cell Rep. 2021 Sep 14;36(11):109716. doi

Insert

ITGA2-tail-YPet (GFFKR/GAAKR) (ITGA2 Human)

Use

Entry vector for gateway cloning

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pEtv5-Bsd-T2a-Fkbp-3xF-GGSG

Plasmid

#235525

Purpose

Etv5 N-term targeting vector, Long Isoform, Blasto-T2A

Depositor

Brian Hendrich

Insert

Etv5 (Etv5 Mouse)

Use

Mouse Targeting

Tags

Bsdd, FKBP, 3xFLAG

Mutation

None

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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Ec holA(Y316A)-FLAG-pET(11a)(ACH-)

Plasmid

#238346

Purpose

over-expresses C-terminal FLAG tagged E. coli delta (Y316A) in E. coli

Depositor

Michael O'Donnell

Insert

C-terminal FLAG tagged E. coli delta (Y316A) (holA E. coli)

Tags

3X FLAG

Expression

Bacterial

Mutation

Y316A

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet1_6xHis-TEV-mCherry-WIPI2d (K87A/K88A)

Plasmid

#223751

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

WIPI2d (WIPI2 Human)

Tags

6xHis-TEV-mCherry

Expression

Bacterial

Mutation

K87A/K88A

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pET28 MBP-TEV-Rat BCKDK-His

Plasmid

#232119

Purpose

For bacterial expression of His-tagged Rat BCKDK (AA31-412, missing precursor peptide) with a cleavable MBP purification tag.

Depositor

Ruth Sommese

Article

Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi

Insert

BCKDK (Bckdk Rat)

Tags

6 x His, Maltose-Binding Protein (MBP), and Tev S…

Expression

Bacterial

Promoter

T7 Promoter

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pETcon-pGAL1-Aga2-Amyloid β42-c_myc

Plasmid

#225223

Purpose

Yeast optimized human Amyloid β42 fused with aga2 protein gene under gal1 promoter for the expression of Amyloid β42 in yeast surface display.

Depositor

Urartu Seker

Article

Ozcelik et al ACS Chem Neurosci. 2023 Oct 4;14(19):360

Inserts

Amyloid β42
Aga2 (AGA2 Budding Yeast)

Tags

HA Tag and c-myc Tag

Expression

Yeast

Promoter

GAL1

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pETcon-pGAL1-Aga2-Htt(25Q)-c_myc

Plasmid

#225228

Purpose

Yeast optimized human Htt (25Q) fused with aga2 protein gene under gal1 promoter for the expression of Htt (25Q) in yeast surface display.

Depositor

Urartu Seker

Article

Khan et al ACS Med Chem Lett. 2023 Nov 14;14(12):18

Inserts

Aga2
Htt (25Q)

Tags

HA Tag and c-myc Tag

Expression

Yeast

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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