We narrowed to 14,590 results for: pET

1. pET28a-GST-BTLAicd (AA190-289, Y257F, Y282F)-TwinStrep

   Plasmid

   #180811

   Purpose

   Expression of GST fused human BTLA intracellular domain (AA190-289, Y257F, Y282F)-TwinStrep

   Depositor

   Enfu Hui

   Article

   Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   GST-BTLAint (AA190-289, Y257F, Y282F)-TwinStrep (BTLA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   BTLA (Y257F, Y282F)

   Promoter

   T7

   Available since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETDuet1-MBP-NAAEF-PNR(217-410 C275)-His6

   Plasmid

   #177849

   Purpose

   Bacterial Expression of PNR

   Depositor

   Karsten Melcher

   Article

   Tan et al PLoS One. 2013 Sep 12;8(9):e74359. doi:

   Insert

   photoreceptor cell-specific nuclear receptor (NR2E3 Human)
   

   Use

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p36-p37-p38-p40-pET-Duet-1-Rfc3-T76D

   Plasmid

   #175051

   Purpose

   Overexpression of human Rfc2,Rfc3-3KtoA,Rfc4,Rfc5 in E.coli

   Depositor

   Brian Kelch

   Article

   Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

   Insert

   Replication factor C subunit 5 (RFC2, RFC3, RFC4, RFC5 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changed Thr76 to Asp, phosphomimetic mutation

   Promoter

   T7 promoter

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. p36-p37-p38-p40-pET-Duet-1-Rfc3-3KtoA

   Plasmid

   #175050

   Purpose

   Overexpression of human Rfc2,Rfc3-3KtoA,Rfc4,Rfc5 in E.coli

   Depositor

   Brian Kelch

   Article

   Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

   Insert

   Replication factor C subunits 2-5, Rfc3-3KtoA-mutation (RFC4, RFC2, RFC3, RFC5 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-ybbr-ELP-ddFLN4-XMod-Doc-HIS (BMB-KO)

   Plasmid

   #153441

   Purpose

   E. coli expression of Rc. XDocB binding mode B knock-out construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.

   Depositor

   Michael Nash

   Article

   Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

   Insert

   Rc. XDocB binding mode B knock-out mutant
   

   Use

   Tags

   3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…

   Expression

   Bacterial

   Mutation

   Arginine 140 and methionine 144 were mutated to a…

   Promoter

   T7 promoter

   Available since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-ybbr-ELP-ddFLN4-XMod-Doc-HIS (BMA-KO)

   Plasmid

   #153440

   Purpose

   E. coli expression of Rc. XDocB binding mode A knock-out construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.

   Depositor

   Michael Nash

   Article

   Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

   Insert

   Rc. XDocB binding mode A knock-out mutant
   

   Use

   Tags

   3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…

   Expression

   Bacterial

   Mutation

   Arginine 191 and leucine 195 on XDocB were mutate…

   Promoter

   T7 promoter

   Available since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET His6 Mocr TEV co-transformation cloning vector (13K-O)

   Plasmid

   #48320

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   His6 and Mocr

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET His6 Mocr TEV co-transformation cloning vector (13S-O)

   Plasmid

   #48327

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   His6 and Mocr

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Kv7.4 AD (325-645, C643A, Δ 368-492)/I346A/pET28HMT

   Plasmid

   #111398

   Purpose

   Protein expression in E.Coli

   Depositor

   Dan Minor

   Article

   Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

   Insert

   potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
   

   Use

   Tags

   His Tag and MBP Tag

   Expression

   Bacterial

   Mutation

   Kv7.4 AD (325-645, C643A, Δ 368-492)/I346A

   Promoter

   T7

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Kv7.4 AD (325-645, C643A, Δ 368-492)/I346D/pET28HMT

    Plasmid

    #111399

    Purpose

    Protein expression in E.Coli

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
    

    Use

    Tags

    His Tag and MBP Tag

    Expression

    Bacterial

    Mutation

    Kv7.4 AD (325-645, C643A, Δ 368-492)/I346D

    Promoter

    T7

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET-Duet-ECHA(37-763) K351R-FLAG (no His-tag)

    Plasmid

    #96888

    Purpose

    expresses-flag tagged mouse ECHA(37-768) K351R in E.coli cells (no His-tag)

    Depositor

    Hening Lin

    Article

    Sadhukhan et al Proc Natl Acad Sci U S A. 2016 Apr 19;11

    Insert

    Trifunctional enzyme subunit alpha, mitochondrial (Hadha Mouse)
    

    Use

    Tags

    FLAG-tagged

    Expression

    Bacterial

    Mutation

    deleted amino acid 1-36, mutated lysine 351 to ar…

    Promoter

    T7

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET His10 MBP Asn10 TEV LIC cloning vector (2CT-10)

    Plasmid

    #55209

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    10xAsn, 10xHis, MPB, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET His6 TEV expression vector with BioBrick polypromoter restriction sites (14-B)

    Plasmid

    #48308

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET His6 TEV cloning vector with BioBrick polycistronic restriction sites (9B)

    Plasmid

    #48284

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET MBP TEV expression vector with BrioBrick polypromoter restriction sites (14-Q)

    Plasmid

    #48311

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. M63 pPET28a-TEV-human beta cat domain C(666-781)

    Plasmid

    #17204

    Depositor

    Randall Moon

    Insert

    beta catenin (CTNNB1 Human)
    

    Use

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    carboxy-terminal residues 666-781

    Promoter

    Available since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

17. pET StrepII TEV cloning vector with BioBrick polycistronic restriction sites (9R)

    Plasmid

    #48290

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    StrepII

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET His6 High pI protein 1 TEV LIC cloning vector (2-HpI-1)

    Plasmid

    #55210

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis, High pI protein 1, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET StrepII TEV expression vector with BioBrick polypromoter restriction sites (14-R)

    Plasmid

    #48312

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    StrepII

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET His6 High pI protein 85 TEV LIC cloning vector (2-HpI-85)

    Plasmid

    #55213

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis, High pI protein 85, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge