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MK11_HUMAN_D0

Plasmid

#79692

Purpose

This plasmid encodes the kinase domain of MK11. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

MK11 (MAPK11 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
FES_HUMAN_D0

Plasmid

#79686

Purpose

This plasmid encodes the kinase domain of FES. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

FES (FES Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
HNF4A_P2-226

Plasmid

#31064

Depositor

Gerhart Ryffel

Article

Thomas et al Hum Mol Genet. 2001 Sep 15. 10(19):2089-

Insert

HNF4A P2 promoter (HNF4A Human)

Use

Luciferase

Expression

Mammalian

Mutation

-226 to -1 of P2 HNF4A promoter

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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MAPK3_HUMAN_D0

Plasmid

#79702

Purpose

This plasmid encodes the kinase domain of MAPK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

MAPK3 (MAPK3 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pBullet-pm-n

Plasmid

#53077

Purpose

destination vector with PIP2a-ECFP (plasma membrane marker) for tagged fluorescent protein (N-ter-EYFP) of plant gene

Depositor

Joshua Heazlewood

Article

Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

Type

Empty backbone

Use

Synthetic Biology

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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pBullet-vac-n

Plasmid

#53075

Purpose

destination vector with vacuole marker (gamma-TIP) - ECFP for tagged fluorescent protein (N-ter-EYFP) of plant gene

Depositor

Joshua Heazlewood

Article

Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

Type

Empty backbone

Use

Synthetic Biology

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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pCAGGS-GFP-WTUBA7-IRES-mcherry

Plasmid

#246475

Purpose

Mammalian expression of AcGFP-tagged WT human UBA7, co-expresses mCherry; for transfection

Depositor

Francisco Bustos

Article

Bandi et al iScience 2026. doi: 10.1016/j.isci.2026.

Insert

AcGFP-WTUBA7-IRES-mcherry (UBA7 Human)

Tags

AcGFP1

Expression

Mammalian

Available Since

April 1, 2026

Availability

Academic Institutions and Nonprofits only

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pCAGGS-GFP-UBA7-V548L-IRES-mcherry

Plasmid

#246476

Purpose

Mammalian expression of AcGFP-tagged human UBA7-V548L, co-expresses mCherry; for transfection

Depositor

Francisco Bustos

Article

Bandi et al iScience 2026. doi: 10.1016/j.isci.2026.

Insert

AcGFP-UBA7-V548L-IRES-mcherry (UBA7 Human)

Tags

AcGFP1

Expression

Mammalian

Mutation

V548L

Available Since

April 1, 2026

Availability

Academic Institutions and Nonprofits only

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pCAGGS-GFP-UBA7-K709Sfs-IRES-mcherry

Plasmid

#246477

Purpose

Mammalian expression of AcGFP-tagged human UBA7-K709Sfs, co-expresses mCherry; for transfection

Depositor

Francisco Bustos

Article

Bandi et al iScience 2026. doi: 10.1016/j.isci.2026.

Insert

AcGFP-UBA7-K709S*fs-IRES-mcherry (UBA7 Human)

Tags

AcGFP1

Expression

Mammalian

Mutation

K709Sfs*

Available Since

April 1, 2026

Availability

Academic Institutions and Nonprofits only

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pHalo2X-mMYO7A-ΔSH3-ΔM/F2

Plasmid

#251427

Purpose

Mammalian expression vector for expressing HaloTag-fused mouse MYO7A truncated after the first MyTH4-FERM domain

Depositor

Thomas Friedman, Takushi Miyoshi

Article

Miyoshi et al Nat Commun. 2025 Sep 1;16(1):8149. doi:

Insert

Myosin VIIa (SH3 and M/F2 trancated) (Myo7a Mouse)

Tags

HaloTag

Expression

Mammalian

Mutation

The SH3 and M/F2 domains trancated

Available Since

March 3, 2026

Availability

Academic Institutions and Nonprofits only

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pHalo2X-mMYO7A-RK/AA

Plasmid

#251426

Purpose

Mammalian expression vector for expressing a HaloTag-fused mouse MYO7A mutant that destabilizes the autoinhibitory conformation.

Depositor

Thomas Friedman, Takushi Miyoshi

Article

Miyoshi et al Nat Commun. 2025 Sep 1;16(1):8149. doi:

Insert

Myosin VIIa (RK/AA) (Myo7a Mouse)

Tags

HaloTag

Expression

Mammalian

Mutation

Two missense mutations in the RGSK motif of the s…

Available Since

March 2, 2026

Availability

Academic Institutions and Nonprofits only

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pHalo2X-actin

Plasmid

#251421

Purpose

Mammalian expression vector for expressing human beta-actin with an N-terminal HaloTag

Depositor

Thomas Friedman, Takushi Miyoshi

Article

Miyoshi et al Nat Commun. 2025 Sep 1;16(1):8149. doi:

Insert

beta-actin (ACTB Human)

Tags

HaloTag

Expression

Mammalian

Available Since

March 2, 2026

Availability

Academic Institutions and Nonprofits only

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pMIHP-16167 AU1.Flag.Ollas

Plasmid

#241479

Purpose

AU1.Flag.Ollas Flow Code Plasmid This plasmid is to be used with mRNAs.

Depositor

Adrian Liston

Article

Burton et al Immunity. 2024 Jul 9;57(7):1586-1602.e10

Type

Empty backbone

Use

Retroviral

Tags

AU1, Flag, and Ollas

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pMIHP-16170 AU1.FLAG.V5

Plasmid

#241480

Purpose

AU1.FLAG.V5 Flow Code Plasmid This plasmid is to be used with mRNAs.

Depositor

Adrian Liston

Article

Burton et al Immunity. 2024 Jul 9;57(7):1586-1602.e10

Type

Empty backbone

Use

Retroviral

Tags

AU1, FLAG, and V5

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pMIHP-16173 AU1.HA.Ollas

Plasmid

#241481

Purpose

AU1.HA.Ollas Flow Code Plasmid This plasmid is to be used with mRNAs.

Depositor

Adrian Liston

Article

Burton et al Immunity. 2024 Jul 9;57(7):1586-1602.e10

Type

Empty backbone

Use

Retroviral

Tags

AU1, HA, and Ollas

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pMIHP-16174 AU1.HA.S

Plasmid

#241482

Purpose

AU1.HA.S Flow Code Plasmid This plasmid is to be used with mRNAs.

Depositor

Adrian Liston

Article

Burton et al Immunity. 2024 Jul 9;57(7):1586-1602.e10

Type

Empty backbone

Use

Retroviral

Tags

AU1, HA, and S

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIHP-16176 AU1.HA.V5

Plasmid

#241483

Purpose

AU1.HA.V5 Flow Code Plasmid This plasmid is to be used with mRNAs.

Depositor

Adrian Liston

Article

Burton et al Immunity. 2024 Jul 9;57(7):1586-1602.e10

Type

Empty backbone

Use

Retroviral

Tags

AU1, HA, and V5

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pMIHP-16186 AU1.Ollas.S

Plasmid

#241484

Purpose

AU1.Ollas.S Flow Code Plasmid This plasmid is to be used with mRNAs.

Depositor

Adrian Liston

Article

Burton et al Immunity. 2024 Jul 9;57(7):1586-1602.e10

Type

Empty backbone

Use

Retroviral

Tags

AU1, Ollas, and S

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pMIHP-16188 AU1.OLLAS.V5

Plasmid

#241485

Purpose

AU1.OLLAS.V5 Flow Code Plasmid This plasmid is to be used with mRNAs.

Depositor

Adrian Liston

Article

Burton et al Immunity. 2024 Jul 9;57(7):1586-1602.e10

Type

Empty backbone

Use

Retroviral

Tags

AU1, OLLAS, and V5

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pMIHP-16190 AU1.S.V5

Plasmid

#241486

Purpose

AU1.S.V5 Flow Code Plasmid This plasmid is to be used with mRNAs.

Depositor

Adrian Liston

Article

Burton et al Immunity. 2024 Jul 9;57(7):1586-1602.e10

Type

Empty backbone

Use

Retroviral

Tags

AU1, S, and V5

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge