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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 33,634 results for: CaS;

Showing: 10451 - 10500 of 33634 results

  32. pEMS1710

    Plasmid
    #29296
    Purpose
    Pleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).
    Depositor
    Elizabeth Simpson
    Article
    de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:
    Insert
    Ple238 (TRH Human)
    Use
    Pleiades promoter project [sic, pleaides plieades]
    Tags
    Intron-LacZ-NLS
    Expression
    Mutation
    Promoter
    Available since
    June 10, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits
    29296-plasmid-map-sequence-id-13711

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  33. LentiCRISPRv2-mCherry

    Plasmid
    #99154
    Purpose
    Lentiviral vector encoding sgRNA cloning site + hSpCAS9-P2A-mCherry.
    Depositor
    Agata Smogorzewska
    Inserts
    Cas9
    mCherry
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    EFS-NS
    Available since
    Aug. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    99154-plasmid-map-sequence-id-352449

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  34. pCMV-ABE-P48R

    Plasmid
    #161816
    Purpose
    Expresses ABE-P48R in mammalian cells
    Depositor
    Sangsu Bae
    Insert
    bpNLS-TadA7.10(P48R)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    D10A in S. pyogenes Cas9, TadA mutations describe…
    Promoter
    Available since
    Dec. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    161816-plasmid-map-sequence-id-320046

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  35. AAVS1-SAGFP

    Plasmid
    #127100
    Purpose
    SA-GFP substrate (Addgene #41594) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. SA-GFP cassette contains PGK-puromycin.
    Depositor
    Adam Karpf
    Insert
    SA-GFP reporter
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    127100-plasmid-map-sequence-id-247555

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  36. pUC19-MAFA-PQR-NLS-tdTomato-P2A-puro-PGK-puro

    Plasmid
    #121174
    Purpose
    Donor template for CRISPR-Cas9 mediated generation of MAFA-NLS-tdTomato reporter cell lines
    Depositor
    Timo Otonkoski
    Insert
    MAFA-PQR-NLS-tdTomato-P2A-Puro-PGK-Puro
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    121174-plasmid-map-sequence-id-234993

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  37. pINO4-Pfv-Sapphire-HO

    Plasmid
    #203659
    Purpose
    Yeast vector encoding donor DNA for integration of 40nm GEMs into the HO locus.
    Depositor
    Liam Holt
    Article
    Shu T et et al bioRxiv 2022
    Insert
    PfV
    Use
    CRISPR
    Tags
    Sapphire
    Expression
    Mutation
    Promoter
    Available since
    July 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    203659-plasmid-map-sequence-id-402489

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  38. lentiCRISPRv2_PROX1_1+3

    Plasmid
    #121176
    Purpose
    Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.
    Depositor
    Timo Otonkoski
    Insert
    Cas9-P2A-puro and U6 promoters driven expression of gRNAs
    Use
    CRISPR and Lentiviral
    Tags
    P2A-puro
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    121176-plasmid-map-sequence-id-235001

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  39. CAG-ABEmax-P2A-EGFP-ires-puro

    Plasmid
    #121170
    Purpose
    CAG promoter driven expression of ABEmax base editor, EGFP and Puromycin resistance.
    Depositor
    Timo Otonkoski
    Insert
    ABEmax-P2A-EGFP-ires-puro
    Use
    CRISPR
    Tags
    P2A-EGFP-ires-puro
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    121170-plasmid-map-sequence-id-234985

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  40. plenti guide ds red

    Plasmid
    #128055
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and DsREd from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Oskar Laur
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    128055-plasmid-map-sequence-id-255092

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  41. pBACH2-donor

    Plasmid
    #176357
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Kevin White, Barbara Stranger
    Insert
    BACH2 (BACH2 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    176357-plasmid-map-sequence-id-347870

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  42. pMTF1-donor

    Plasmid
    #113829
    Purpose
    CRISPR donor plasmid to tag human transcription factor MTF1 with GFP
    Depositor
    Kevin White
    Insert
    MTF1 homology arms flanking EGFP-IRES-Neo cassette (MTF1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    113829-plasmid-map-sequence-id-230210

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  43. pCGGBP1-donor

    Plasmid
    #176345
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Kevin White, Barbara Stranger
    Insert
    CGGBP1 (CGGBP1 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available since
    Nov. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    176345-plasmid-map-sequence-id-347266

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  44. TLCV2 sgAAVS1

    Plasmid
    #127099
    Purpose
    Encodes gRNA for human AAVS1.
    Depositor
    Adam Karpf
    Insert
    AAVS1 (PPP1R12C AAVS1 Human) (PPP1R12C Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    U6
    Available since
    June 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    127099-plasmid-map-sequence-id-246497

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  45. pSOX2-donor

    Plasmid
    #176364
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Kevin White, Barbara Stranger
    Insert
    SOX2 (SOX2 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    176364-plasmid-map-sequence-id-347872

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  46. pSNAI2-donor

    Plasmid
    #112378
    Purpose
    CRISPR donor plasmid to tag human transcription factor SNAI2 with GFP
    Depositor
    Kevin White
    Insert
    SNAI2 homology arms flanking EGFP-IRES-Neo cassette (SNAI2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112378-plasmid-map-sequence-id-229744

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  47. CAG-FNLS-T2A-EGFP-ires-puro

    Plasmid
    #121169
    Purpose
    CAG promoter driven expression of FNLS BE3 base editor, EGFP and Puromycin resistance.
    Depositor
    Timo Otonkoski
    Insert
    FNLS-T2A-EGFP-ires-puro
    Use
    CRISPR
    Tags
    T2A-EGFP-ires-puro
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    121169-plasmid-map-sequence-id-235002

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  48. pZBTB9-donor

    Plasmid
    #112373
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZBTB9 with GFP
    Depositor
    Kevin White
    Insert
    ZBTB9 homology arms flanking EGFP-IRES-Neo cassette (ZBTB9 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Unknown
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112373-plasmid-map-sequence-id-229732

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  49. pID3-donor

    Plasmid
    #176342
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Kevin White, Barbara Stranger
    Insert
    ID3 (ID3 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    176342-plasmid-map-sequence-id-347890

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  50. pZNF436-donor

    Plasmid
    #113800
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF436 with GFP
    Depositor
    Kevin White
    Insert
    ZNF436 homology arms flanking EGFP-IRES-Neo cassette (ZNF436 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    rs2849028, rs646193
    Promoter
    Available since
    Dec. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    113800-plasmid-map-sequence-id-229883

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Showing: 10451 - 10500 of 33634 results