We narrowed to 2,485 results for: organization Addgene

1. pcDNA 3.1 AURKA Lys162Met

   Plasmid

   #157751

   Purpose

   Expression of kinase-dead AURKA K62M in mammalian cells

   Depositor

   Marc Tramier

   Article

   Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

   Insert

   AURKA (AURKA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   AURKA K162M is a kinase-dead version of AURKA

   Promoter

   CMV

   Available since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3/flag-pbira(1-g78)-sec61b

   Plasmid

   #154455

   Purpose

   expresses flag-pbira(1-g78)-sec61b/biochemical assay

   Depositor

   Hyun-Woo Rhee

   Article

   Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

   Insert

   flag-pbira(1-g78)-sec61b (SEC61B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promoter

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-HF1RA-P2A-GFP-PGK-Puro

   Plasmid

   #110866

   Purpose

   Lentiviral vector for constitutive expression of Cas9-HF1RA-P2A-GFP in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-HF1RA
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   R661A, Q695A, Q926A, and NLS sequence at the N-te…

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-xFNLS-P2A-Puro

   Plasmid

   #110872

   Purpose

   Lentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   xFNLS(3.7)
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Expression

   Mutation

   D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

   Promoter

   EF1s

   Available since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-xBE4Gam-P2A-Puro

   Plasmid

   #112676

   Purpose

   Lentivirus for constitutive expression of xCas9(3.7)-BE4Gam in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   xCas9(3.7)-BE4Gam
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

   Promoter

   EF1s

   Available since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-xBE3-P2A-Puro

   Plasmid

   #110870

   Purpose

   Lentiviral vector for constitutive expression of xCas9(3.7)-BE3 in mammalian cells

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   xCas9(3.7)-BE3
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT2K-CAGGS-mouse Gfi1-IRES-GFP

   Plasmid

   #91893

   Purpose

   Mammalian expression of mouse Gfi1

   Depositor

   Martine Roussel

   Article

   Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

   Insert

   Gfi1 (Gfi1 Mouse)
   

   Use

   Tags

   IRES-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAGGS

   Available since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

8. pPB CAG Cdk6 R31C-IRES-EGFP

   Plasmid

   #153946

   Purpose

   piggyBac transposon vector with CAG promoter expressing Cdk6 (R31C mutant) and EGFP

   Depositor

   Luca Tiberi

   Article

   Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

   Insert

   Cdk6 (Cdk6 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R31C

   Promoter

   CAG

   Available since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPB CAG Gfi1:FLAG-IRES-EGFP

   Plasmid

   #153945

   Purpose

   piggyBac transposon vector with CAG promoter expressing FLAG-tagged Gfi1 and EGFP

   Depositor

   Luca Tiberi

   Article

   Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

   Insert

   Gfi1 (Gfi1 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP N1 mitoAURKA

    Plasmid

    #157754

    Purpose

    Expression of AURKA:GFP targeted to mitochondria

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    AURKA without the first 30 amino acids replaced b…

    Promoter

    CMV

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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