Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 13,862 results for: cas9

Showing: 8441 - 8480 of 13862 results

  1. GE-0010-gRNA2-MpCKB

    Plasmid
    #238528
    Purpose
    Plant expression of Cas9 and gRNA against M. polymorpha CK2 beta
    Depositor
    Michael Hothorn
    Insert
    MpCKB
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available Since
    June 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    238528-plasmid-map-sequence-id-477293

    Enlarge

  2. GE-0010-gRNA1-MpCKB

    Plasmid
    #238527
    Purpose
    Plant expression of Cas9 and gRNA against M. polymorpha CK2 beta
    Depositor
    Michael Hothorn
    Insert
    MpCKB
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available Since
    June 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    238527-plasmid-map-sequence-id-477292

    Enlarge

  3. GE-0010-gRNA1-MpCKA

    Plasmid
    #238526
    Purpose
    Plant expression of Cas9 and gRNA against M. polymorpha CK2 alpha
    Depositor
    Michael Hothorn
    Insert
    MpCKA
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available Since
    June 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    238526-plasmid-map-sequence-id-477280

    Enlarge

  4. pPATZ1.1.0-gDNA

    Plasmid
    #132476
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    PATZ1 (PATZ1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    132476-plasmid-map-sequence-id-267188

    Enlarge

  5. pZNF449-R.1.0-gDNA

    Plasmid
    #132469
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    ZNF449 (ZNF449 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    132469-plasmid-map-sequence-id-267138

    Enlarge

  6. pZNF302.1.0-gDNA

    Plasmid
    #132467
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    ZNF302 (ZNF302 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    132467-plasmid-map-sequence-id-267123

    Enlarge

  7. pZNF253.1.0-gDNA

    Plasmid
    #132461
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    ZNF253 (ZNF253 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    132461-plasmid-map-sequence-id-267028

    Enlarge

  8. pZFPM2.1.0-gDNA

    Plasmid
    #132459
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    ZFPM2 (ZFPM2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    132459-plasmid-map-sequence-id-266993

    Enlarge

  9. pZNF3.1.0-gDNA

    Plasmid
    #132440
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    ZNF3 (ZNF3 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    132440-plasmid-map-sequence-id-266296

    Enlarge

  10. pTSHZ1.1.0-gDNA

    Plasmid
    #132434
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    TSHZ1 (TSHZ1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    132434-plasmid-map-sequence-id-266216

    Enlarge

  11. C41m

    Plasmid
    #120974
    Purpose
    MoClo golden gate assembly CD part for dCas9 (Catalytically-inactive S. pyogenes Cas9, for gRNA-targeted repression). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    dCas9
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120974-plasmid-map-sequence-id-236028

    Enlarge

  12. pICSL4723_ HDT701

    Plasmid
    #126886
    Purpose
    Genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 module, nptII resistance gene, and specific sgRNA modules on the binary plasmid pICSL4723.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    July 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    126886-plasmid-map-sequence-id-249696

    Enlarge

  13. pENTRY4_Rac5

    Plasmid
    #126897
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    July 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    126897-plasmid-map-sequence-id-250096

    Enlarge

  14. pENTRY4_ OBfold

    Plasmid
    #126901
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    126901-plasmid-map-sequence-id-249798

    Enlarge

  15. pICSL4723_ OBfold

    Plasmid
    #126889
    Purpose
    Genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 module, nptII resistance gene, and specific sgRNA modules on the binary plasmid pICSL4723.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    126889-plasmid-map-sequence-id-249747

    Enlarge

  16. pENTRY4_ HDT701

    Plasmid
    #126898
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    126898-plasmid-map-sequence-id-249718

    Enlarge

  17. pENTRY4_ ERF922

    Plasmid
    #126893
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    126893-plasmid-map-sequence-id-249692

    Enlarge

  18. PX458_PBX2_1

    Plasmid
    #72357
    Purpose
    Encodes gRNA for 3' target of human PBX2 along with Cas9 with 2A GFP
    Depositor
    Richard M. Myers, Eric Mendenhall
    Insert
    PBX2 (PBX2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Feb. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    72357-plasmid-map-sequence-id-132107

    Enlarge

  19. PX458_PBX2_2

    Plasmid
    #72358
    Purpose
    Encodes gRNA for 3' target of human PBX2 along with Cas9 with 2A GFP
    Depositor
    Richard M. Myers, Eric Mendenhall
    Insert
    PBX2 (PBX2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Feb. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    72358-plasmid-map-sequence-id-132108

    Enlarge

  20. lenti MS2-P65-HSF1_Hygro

    Plasmid
    #61426
    Purpose
    lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker NOTE: A version of this plasmid with improved titer is available: Addgene plasmid #89308 lentiMPH v2
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Insert
    MS2-P65-HSF1_2A_Hygro (HSF1 Human, Synthetic)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    N55K in MS2
    Promoter
    EF1A
    Available Since
    Dec. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    61426-plasmid-map-sequence-id-140565

    Enlarge

  27. pCMV-T7-SpRY-HF1-P2A-EGFP (RTW5008)

    Plasmid
    #139997
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpRY-HF1(SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9 variant named SpRY-HF1 with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…
    Promoter
    CMV and T7
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139997-plasmid-map-sequence-id-352446

    Enlarge

  33. pCMV-PE6g

    Plasmid
    #207857
    Purpose
    Mammalian expression of PE6g prime editor
    Depositor
    David Liu
    Article
    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.
    Insert
    PE6g
    Use
    Tags
    SV40 bpNLS and SV40 bpNLS, c-Myc NLS
    Expression
    Mammalian
    Mutation
    Cas9(H840A, E471K, H99R, I632V, H721Y, R654C, D64…
    Promoter
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    207857-plasmid-map-sequence-id-408164

    Enlarge

  34. PB-GFP-Gal8

    Plasmid
    #127191
    Purpose
    Piggybac transposon plasmid with CAG promoter GFP-Galectin 8 (GFP-Gal8) fusion protein. Useful as genetically encoded endosomal escape sensor.
    Depositor
    Jordan Green
    Article
    Rui et al Sci Adv. 2019 Dec 6;5(12):eaay3255. doi:
    Insert
    GFP-Gal8 (LGALS8 Human)
    Use
    Tags
    Gal8 is fused to the c-terminus of GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Dec. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    127191-plasmid-map-sequence-id-248359

    Enlarge

  35. pCMV-PE6f

    Plasmid
    #207856
    Purpose
    Mammalian expression of PE6f prime editor
    Depositor
    David Liu
    Article
    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.
    Insert
    PE6f
    Use
    Tags
    SV40 bpNLS and SV40 bpNLS, c-Myc NLS
    Expression
    Mammalian
    Mutation
    Cas9(H840A, E471K, H99R, I632V, H721Y, D645N, K91…
    Promoter
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    207856-plasmid-map-sequence-id-408168

    Enlarge

  40. pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025)

    Plasmid
    #140003
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized ABEmax(7.10) SpCas9 variant named SpRY with P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…
    Promoter
    CMV and T7
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140003-plasmid-map-sequence-id-337354

    Enlarge

Showing: 8441 - 8480 of 13862 results