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pCMV-SEMPER_TTT-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

Plasmid

#221079

Purpose

SEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication

Depositor

Mikhail Shapiro

Article

Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

Insert

TTT-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAV-puro-U6+27-F30-Tornado-Squash-FTL-IRE

Plasmid

#216828

Purpose

Expresses a circular RNA containing a Squash aptamer and an IRE (FTL derived) in mammalian cells, using the Tornado expression system.

Depositor

Samie Jaffrey

Article

Hagen et al Cell Chem Biol. 2024 Aug 23:S2451-9456(2

Insert

Tornado-Squash-FTL-IRE

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6+27

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAV-puro-U6+27-F30-Tornado-Squash-TfR-IRE

Plasmid

#216829

Purpose

Expresses a circular RNA containing a Squash aptamer and an IRE (TfR derived) in mammalian cells, using the Tornado expression system.

Depositor

Samie Jaffrey

Article

Hagen et al Cell Chem Biol. 2024 Aug 23:S2451-9456(2

Insert

Tornado-Squash-TfR-IRE

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6+27

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD H1734A H1741A

Plasmid

#169830

Purpose

Expresses C-terminal flag-tagged CAD with mutations at reported dimerization interface of the DHOase domain

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

H1734A H1741A; TCCC -> AGTC silent mutations a…

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD delta Loop1

Plasmid

#169831

Purpose

Expresses C-terminal flag-tagged CAD with deletion of amino acids F1366 - P1376 in allosteric domain

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

Deletion of amino acids F1366 - P1376; TCCC ->…

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD delta Loop2

Plasmid

#169832

Purpose

Expresses C-terminal flag-tagged CAD with deletion of amino acids R1398 - S1407 in allosteric domain

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

Deletion of amino acids R1398 - S1407; TCCC ->…

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD T456A S1406A

Plasmid

#169823

Purpose

Expresses C-terminal flag-tagged CAD with mutation of reported Erk1/2 and PKA phosphorylation site

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

T456A S1406A; TCCC -> AGTC silent mutations at…

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD T456A S1859A

Plasmid

#169824

Purpose

Expresses C-terminal flag-tagged CAD with mutation of reported Erk1/2 and S6K phosphorylation site

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

T456A S1859A; TCCC -> AGTC silent mutations at…

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pLV UBC OSBP(PH)-APEX2-3xFLAG-IRES-eGFP

Plasmid

#218637

Purpose

Proximity biotinylation control of OSBP(PH domain)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

OSBP (OSBP Human)

Use

Lentiviral

Tags

APEX2-3xFLAG

Expression

Mammalian

Mutation

PH domain only

Promoter

UBC

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pEF-IRES-puro6 gal4DBD-HIFCAD myc tag

Plasmid

#207171

Purpose

expression plasmid for Gal4DBD-HIF-CAD.myc expression

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Insert

Gal4DBD-HIF1a-CAD Myc tag linker

Use

Tags

2xMyc tag

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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