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We narrowed to 2,369 results for: PUC19

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Showing: 2121 - 2130 of 2369 results

  1. qTAG-C-Solo-mStayGold-MAPRE1

    Plasmid
    #227324
    Purpose
    Donor template for mStayGold insertion into the C-terminus of the MAPRE1 locus. For growing microtubule tip visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MAPRE1 (Addgene #207793)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    MAPRE1 Homology Arms flanking a mStayGold Tag (MAPRE1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227324-plasmid-map-sequence-id-452106

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  2. qTAG-N-Solo-mStayGold-CEP135

    Plasmid
    #227287
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the CEP135 locus. For centriole visualization. To be co-transfected with sgRNA plasmid px330-CEP135 (Addgene #227286)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    CEP135 Homology Arms flanking a mStayGold Tag (CEP135 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227287-plasmid-map-sequence-id-452057

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  3. qTAG-N-Solo-mStayGold-GOLGA2

    Plasmid
    #227325
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the GOLGA2 locus. For Golgi visualization. To be co-transfected with sgRNA plasmid px330-PITCh-GOLGA2 (Addgene #207791)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    GOLGA2 Homology Arms flanking a mStayGold Tag (GOLGA2 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227325-plasmid-map-sequence-id-452110

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  4. IR25a-T2A-QF-AD

    Plasmid
    #162526
    Purpose
    Plasmid for CRISPR-mediated knock-in line that expresses the activation domain of QF by knocking-in a T2A-QF-AD fragment replacing the stop codon of the endogenous Aedes aegypti Ir25a gene
    Depositor
    Leslie Vosshall
    Article
    Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.
    Insert
    Ir25a-left-HDR-arm; T2A-QF2AD-Zip+-SV40; 3xP3-EYFP-SV40; Ir25a-right-HDR-arm
    Expression
    Insect
    Available Since
    Aug. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    162526-plasmid-map-sequence-id-354666

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  6. qTAG-C-mStayGold-Puro-CANX

    Plasmid
    #227281
    Purpose
    Donor template for mStayGold-2A-Puro insertion into the C-terminus of the CANX locus. For cilia visualization. To be co-transfected with sgRNA plasmid px330-CANX (Addgene #227279)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    CANX Homology Arms flanking a mStayGold-2A-Puro Cassette (CANX Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227281-plasmid-map-sequence-id-452050

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  7. pNOC_hfnCas12a_CRATES

    Plasmid
    #176251
    Purpose
    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and type IIS restriction site for endonuclease BaeI at the crRNA region that facilitates the generation of mulitpl
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    humanized fnCas12a
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176251-plasmid-map-sequence-id-347017

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  8. pMT899

    Plasmid
    #228288
    Purpose
    Constitutive mutant rPhlTA expression with strong promoter
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi
    Insert
    rphlTA mutant
    Use
    Synthetic Biology
    Tags
    Three copies of VP16 (VP48)-Nuclear localization …
    Expression
    Yeast
    Mutation
    P5S, S6P, K86T, F109L, Q117R, E143K
    Promoter
    KpGAPDH promoter
    Available Since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228288-plasmid-map-sequence-id-457110

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  9. qTAG-N-Blast-mNeon-ACTB

    Plasmid
    #207751
    Purpose
    Donor template for Blast-2A-mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    ACTB Homology Arms flanking a Blast-mNeon Cassette (ACTB Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    207751-plasmid-map-sequence-id-416864

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  10. qTAG-N-Puro-moxGFP-LMNB1

    Plasmid
    #207773
    Purpose
    Donor template for Puro-2A-moxGFP insertion into the N-terminus of the LMNB1 locus for nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    LMNB1 Homology Arms flanking a Puro-moxGFP Cassette (LMNB1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207773-plasmid-map-sequence-id-413797

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Showing: 2121 - 2130 of 2369 results