We narrowed to 4,705 results for: golden gate

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pEvolvR-mCherry-SpRY-nCas9(D10A)-PolI5MΔ

Plasmid

#249074

Purpose

Expresses nucleus-localized SpRY-nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.

Depositor

John Dueber, David Schaffer

Article

Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:

Insert

SpRY-nCas9-PolI5MΔ

Use

CRISPR

Tags

mCherry

Expression

Mammalian

Mutation

deletion of PolI5M N-terminal flap endonuclease d…

Promoter

CMV

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pX330-TAF1-A

Plasmid

#247346

Purpose

Expresses SpCas9 and a sgRNA targeting the human TAF1-A loci for knock-in.

Depositor

Kazuhiro Maeshima

Article

Ide et al Sci Adv. 2020 Oct 14;6(42). pii: 6/42/ea

Insert

TAF1 (TAF1 Human)

Use

CRISPR

Promoter

U6

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pMK024

Plasmid

#236571

Purpose

Cloning vector for PaqCI Golden Gate assembly of ISAam1 guides into ISAam1 plant expression vector

Depositor

Steven Jacobsen

Article

Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

Type

Empty backbone

Expression

Plant

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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L4278 hIgKLSS_hTGFb1 in pcDNA backbone

Plasmid

#244184

Purpose

Constitutive expression of human TGF-beta1 (with the human IgK light chain secretion sequence)

Depositor

Joshua Leonard

Article

Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

Insert

TGF-beta1 with IgK light chain secretion sequence (TGFB1 Synthetic, Human)

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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L4275 hIgKLSS_hTNF in pcDNA backbone

Plasmid

#244182

Purpose

Constitutive expression of human mature TNF (with the human IgK light chain secretion sequence)

Depositor

Joshua Leonard

Article

Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

Insert

TNF with IgK light chain secretion sequence (TNF Synthetic, Human)

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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LLP984

Plasmid

#239906

Purpose

TCTP-SynPro-04 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

TCTP-SynPro-04 engineered promoter

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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LLP985

Plasmid

#239907

Purpose

TCTP-SynPro-05 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

TCTP-SynPro-05 engineered promoter

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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LLP1022

Plasmid

#239944

Purpose

Firefly luciferase plus as a Level-0 part (Golden Gate)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

Fluc

Use

Synthetic Biology

Tags

PEST on C-terminus of Fluc plus

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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LLP1013

Plasmid

#239935

Purpose

Csy4 recognition sequences (Cog) as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

Csy4 recognition sequence

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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LLP982

Plasmid

#239904

Purpose

TCTP-SynPro-02 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

TCTP-SynPro-02 engineered promoter

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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LLP989

Plasmid

#239911

Purpose

TCTP-SynPro-09 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

TCTP-SynPro-09 engineered promoter

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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LLP990

Plasmid

#239912

Purpose

TCTP-SynPro-10 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

TCTP-SynPro-10 engineered promoter

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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LLP1021

Plasmid

#239943

Purpose

Renilla luciferase as a Level-0 part (Golden Gate)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

Rluc

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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LLP981

Plasmid

#239903

Purpose

TCTP-SynPro-01 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

TCTP-SynPro-01 engineered promoter

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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LLP983

Plasmid

#239905

Purpose

TCTP-SynPro-03 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

TCTP-SynPro-03 engineered promoter

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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LLP986

Plasmid

#239908

Purpose

TCTP-SynPro-06 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

TCTP-SynPro-06 engineered promoter

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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LLP987

Plasmid

#239909

Purpose

TCTP-SynPro-07 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

TCTP-SynPro-07 engineered promoter

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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LLP988

Plasmid

#239910

Purpose

TCTP-SynPro-08 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

TCTP-SynPro-08 engineered promoter

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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LLP1010

Plasmid

#239932

Purpose

dCas9 with Stop codon as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

dCas9

Use

CRISPR and Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pKG3658_pPV2-NLS-FXBP-ZF43-P2A-NES-VP64-FRB-P2A-iRFP720

Plasmid

#239699

Purpose

BsaI Golden Gate assembly plasmid encoding the COMET NLS-FXBP-ZF43-P2A-NES-VP64-FRB-P2A-iRFP720 CDS

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

NLS-FXBP-ZF43-P2A-NES-VP64-FRB-P2A-iRFP720

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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