We narrowed to 13,862 results for: cas9

1. pLenti-VQR-P2A-GFP-PGK-Puro

   Plasmid

   #110861

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VQR-P2A-GFP (not codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VQR
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Expression

   Mutation

   D1135V, R1335Q, T1337R

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PINK1 gRNA (BRDN0001147554)

   Plasmid

   #78035

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PINK1

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PINK1 (PINK1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   June 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PINK1 gRNA (BRDN0001145357)

   Plasmid

   #78036

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PINK1

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PINK1 (PINK1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. FXN gRNA (BRDN0001144784)

   Plasmid

   #77813

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human FXN

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   FXN (FXN Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. FXN gRNA (BRDN0001145648)

   Plasmid

   #77814

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human FXN

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   FXN (FXN Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. FXN gRNA (BRDN0001146360)

   Plasmid

   #77815

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human FXN

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   FXN (FXN Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. ERN1 gRNA (BRDN0001162231)

   Plasmid

   #76409

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human ERN1

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   ERN1 (ERN1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AAV_Actb HR donor

   Plasmid

   #97317

   Purpose

   HR donor for fusing a p2A-mCherry reporter to mouse Actb.

   Depositor

   Hui Yang

   Article

   Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

   Insert

   Actb HR donor
   

   Use

   AAV and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Lenti-pHSE::Cre-mRuby3

   Plasmid

   #164136

   Purpose

   Lentiviral construct for building stable cell lines expressing Cre recombinase and mRuby3 in the presence of heat induction

   Depositor

   Hyongbum Kim

   Article

   Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

   Insert

   HSE promoter-Cre-T2A-mRuby3
   

   Use

   CRISPR, Cre/Lox, Lentiviral, and Synthetic Biolog…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   HSE synthetic promoter

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJEP13-AAV-H1/TO-L-sgRNA(Tet2)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid

    #82703

    Purpose

    AAV backbone with a full length H1/TO promoter driving expression of a sgRNA that targets exon 3 of the mouse Tet2 locus. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.

    Depositor

    Jonathan Ploski

    Article

    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do

    Insert

    H1/TO-L gRNA Expression Cassette Containing Tet2 gRNA
    

    Use

    AAV and CRISPR

    Tags

    CMV promoter driving expression of TetR-P2A-GFP-K…

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. SRC gRNA (BRDN0001145402)

    Plasmid

    #75650

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SRC

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    SRC (SRC Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Chr7_Centromere-Targeting_gRNA

    Plasmid

    #195129

    Purpose

    dual gRNA vector targeting centromere-proximal locations on Chromosome 7p and 7q in a third generation Cas9 backbone with GFP

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    Chr7 gRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. DGKQ gRNA (BRDN0001162275)

    Plasmid

    #77377

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human DGKQ

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    DGKQ (DGKQ Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. MAP3K3 gRNA (BRDN0001148097)

    Plasmid

    #76016

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MAP3K3

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MAP3K3 (MAP3K3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. SRC gRNA (BRDN0001149457)

    Plasmid

    #75652

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SRC

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    SRC (SRC Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AKT1 gRNA (BRDN0001149414)

    Plasmid

    #75502

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human AKT1

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    AKT1 (AKT1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ATM gRNA (BRDN0001149518)

    Plasmid

    #77529

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ATM

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ATM (ATM Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SRC gRNA (BRDN0001146185)

    Plasmid

    #75651

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SRC

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    SRC (SRC Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Chr1q_Cassette-Integration_gRNA

    Plasmid

    #195126

    Purpose

    gRNA in a third generation Cas9 vector with GFP, targeting location downstream of Chr1q_centromere-targeting_gRNA for positive-negative selection cassette integration

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    Chr1q gRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGLOW39

    Plasmid

    #173068

    Purpose

    mScarlet^SEC^3xMyc vector with ccdB sites for cloning homology arms

    Depositor

    Ryan Doonan

    Article

    Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

    Insert

    mScarlet-I-C1^SEC^3xMyc
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xMyc and C. elegans codon-optimized mScarlet

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. CDK16 gRNA (BRDN0001147125)

    Plasmid

    #76903

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CDK16

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CDK16 (CDK16 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. LRPPRC gRNA (BRDN0001145987)

    Plasmid

    #77773

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human LRPPRC

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    LRPPRC (LRPPRC Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. CDK16 gRNA (BRDN0001146694)

    Plasmid

    #76901

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CDK16

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CDK16 (CDK16 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. CDK16 gRNA (BRDN0001147123)

    Plasmid

    #76902

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CDK16

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CDK16 (CDK16 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. MASTL gRNA (BRDN0001148591)

    Plasmid

    #75989

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MASTL

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MASTL (MASTL Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Lenti-sgNeo/Cre

    Plasmid

    #67594

    Purpose

    Expresses an Neomycin-targeting gRNA and Cre-recombinase

    Depositor

    Monte Winslow

    Article

    Chiou et al Genes Dev. 2015 Jul 15;29(14):1576-85. d

    Insert

    sgNeo
    

    Use

    CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. JAK1 gRNA (BRDN0001149025)

    Plasmid

    #76395

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human JAK1

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    JAK1 (JAK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. LRPPRC gRNA (BRDN0001147349)

    Plasmid

    #77774

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human LRPPRC

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    LRPPRC (LRPPRC Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJOP-HTT-HR18Q

    Plasmid

    #87228

    Purpose

    HTT HR arms with 18 CAG repeats and piggyBac selection cassette

    Depositor

    Mahmoud Pouladi

    Article

    Xu et al Stem Cell Reports. 2017 Feb 21. pii: S22

    Insert

    1.7kb HTT 5' homology arm, 2.5kb HTT 3' homology arm
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a driving selection cassette

    Available Since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCMV-hAID-BE3

    Plasmid

    #113417

    Purpose

    Expresses hAID-BE3 in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

    Insert

    hAID-BE3 (AICDA Human, S. pyogenes and Bacteriophage PBS2)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. CDK2 gRNA (BRDN0001147786)

    Plasmid

    #77192

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CDK2

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CDK2 (CDK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. RPS6KA3 gRNA (BRDN0001144943)

    Plasmid

    #75942

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RPS6KA3

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RPS6KA3 (RPS6KA3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. ATM gRNA (BRDN0001149033)

    Plasmid

    #77531

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ATM

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ATM (ATM Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. RPS6KA3 gRNA (BRDN0001148452)

    Plasmid

    #75943

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RPS6KA3

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RPS6KA3 (RPS6KA3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pORANGE GFP-RAB11a KI

    Plasmid

    #131499

    Purpose

    Endogenous tagging of RAB11: N-terminal (amino acid position: before startcodon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Rab11a, Rab11a Mouse, Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. ATM gRNA (BRDN0001146099)

    Plasmid

    #77530

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ATM

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ATM (ATM Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pYLsgRNA-OsU3m/LacZ

    Plasmid

    #66192

    Purpose

    cloning of sgRNAs for expression in plants

    Depositor

    Yao-Guang Liu

    Article

    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

    Type

    Empty backbone

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    OsU3m (SpeI site destroyed)

    Available Since

    Aug. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. EIF2AK4 gRNA (BRDN0001146469)

    Plasmid

    #75876

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human EIF2AK4

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    EIF2AK4 (EIF2AK4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. MAP4K4 gRNA (BRDN0001162353)

    Plasmid

    #76266

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MAP4K4

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MAP4K4 (MAP4K4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. MAP4K4 gRNA (BRDN0001148610)

    Plasmid

    #76263

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MAP4K4

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MAP4K4 (MAP4K4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge