We narrowed to 22,378 results for: CRISPR

1. pSF278

   Plasmid

   #167673

   Purpose

   Intermediate vector for three gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   attL1-ccdB-attL2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. qTAG-C-mStayGold-Blast

   Plasmid

   #227254

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-2A-Blast cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMBP-AsCas12a

   Plasmid

   #113430

   Purpose

   Expresses 10xHis-MBP fused to AsCas12a

   Depositor

   Jennifer Doudna

   Article

   Chen et al Science. 2018 Apr 27;360(6387):436-439.

   Insert

   AsCas12a
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSF463

   Plasmid

   #167671

   Purpose

   Intermediate vector for two gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   attL1-ccdB-attL2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCE059-SiT-Cas12a-[Activ]

   Plasmid

   #128136

   Purpose

   Express both AsCas12a fused to [Activ] activator domain and CRISPR arrays on a single transcript

   Depositor

   Randall Platt

   Article

   Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

   Insert

   AsCas12a-[Activ]-Triplex
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. qTAG-N-Blast-3xFLAG

   Plasmid

   #207684

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-3xFLAG cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pKVL2-U6gRNA_SAM(BbsI)-PGKpuroBFP-W

   Plasmid

   #112925

   Purpose

   Lentiviral vector for SAM-CRISPRa gRNA expression with puroBFP

   Depositor

   Kosuke Yusa

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   hU6 promoter, gRNA-SAM expression cassette
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. qTAG-C-3xHA-Blast

   Plasmid

   #207718

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xHA-2A-Blast cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. qTAG-N-Blast-V5

   Plasmid

   #207686

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-V5 cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. qTAG-N-Puro-dTAG

    Plasmid

    #207694

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-dTAG cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. qTAG-N-Zeo-3xFLAG

    Plasmid

    #207706

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-3xFLAG cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. qTAG-N-Blast-3xHA

    Plasmid

    #207685

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-3xHA cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pLBH559_Tet-HisCas14a1Locus

    Plasmid

    #112502

    Purpose

    Expresses 10xHis-Cas14a1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

    Depositor

    Jennifer Doudna

    Article

    Harrington et al Science. 2018 Oct 18. pii: science.aav42

    Insert

    Cas14a1
    

    Use

    CRISPR

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Tet

    Available since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330A_D10A-1x2

    Plasmid

    #58772

    Purpose

    Expresses Cas9 nickase and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Promoter

    CBh

    Available since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. qTAG-C-ultraID-Puro

    Plasmid

    #207726

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a ultraID-2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. qTAG-C-3xFLAG-Zeo

    Plasmid

    #207739

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xFLAG-2A-Zeo cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. qTAG-C-V5-Blast

    Plasmid

    #207719

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a V5-2A-Blast cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. LentiGuide Cherry

    Plasmid

    #170510

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and mCherry marker from EF-1a promoter. 3rd generation lentiviral backbone.

    Depositor

    Pavel Tolar

    Article

    Newman et al biorXiv 2021.03.02.433398

    Inserts

    S. pyogenes sgRNA cassette
    mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a and hU6

    Available since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pYPQ131B

    Plasmid

    #69281

    Purpose

    Golden Gate entry vector; 1st gRNA under AtU3 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYPQ132B

    Plasmid

    #69282

    Purpose

    Golden Gate entry vector; 2nd gRNA under AtU3 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. qTAG-C-dTAG-Blast

    Plasmid

    #207716

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Blast cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. qTAG-C-ultraID-Blast

    Plasmid

    #207715

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a ultraID-2A-Blast cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. pMJ915

    Plasmid

    #69090

    Purpose

    Express Streptococcus pyogenes Cas9 carrying two C-terminal SV40 NLS

    Depositor

    Jennifer Doudna

    Article

    Lin et al Elife. 2014 Dec 15;3:e04766. doi: 10.755

    Insert

    cas9
    

    Use

    Tags

    MBP, hexa His, and two SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pYPQ133B

    Plasmid

    #69283

    Purpose

    Golden Gate entry vector; 3rd gRNA under AtU3 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. qTAG-C-sTagRFP-Puro

    Plasmid

    #207723

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a sTagRFP-2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. qTAG-C-mScarlet-Puro

    Plasmid

    #207722

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. qTAG-C-mScarlet-Blast

    Plasmid

    #207711

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-2A-Blast cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pMBP-FnCas12a

    Plasmid

    #113432

    Purpose

    Expresses 10xHis-MBP fused to FnCas12a

    Depositor

    Jennifer Doudna

    Article

    Chen et al Science. 2018 Apr 27;360(6387):436-439.

    Insert

    FnCas12a
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJAT31

    Plasmid

    #204291

    Purpose

    For inserting a phiC31 attP site. HDR integrant is marked with ie1-DsRed, which is expressed in abdomen and mouthparts. Second U6 promoter allows use of two different gRNAs in two synthesized arms.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    ie1-DsRed
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    ie1

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSF280

    Plasmid

    #167675

    Purpose

    Intermediate vector for six gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    attL1-ccdB-attL2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge