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Addgene

We narrowed to 33,610 results for: CaS;

Showing: 10751 - 10800 of 33610 results
  1. pAAV hSyn INTRON mEmerald-ER WPRE

    Plasmid
    #236230
    Purpose
    AAV expression of a fluorescent marker, mEmerald fused to Prolactin signal peptide and KDEL sequence; for expression and retention in the lumen of the endoplasmic reticulum
    Insert
    mEmerald-PRL signal peptide-KDEL sequence (PRL Bovine)
    Use
    AAV
    Tags
    mEmerald
    Expression
    Mutation
    Promoter
    human Synapsin 1
    Available since
    May 2, 2025
    Availability
    Academic Institutions and Nonprofits only
  2. GFP Cav2.2 HA EI,II,III,IVA (E314A, E664A, E1370A, E1658A) pcDNA3

    Plasmid
    #206093
    Purpose
    Cav2.2 calcium channel with a N-terminal GFP tag, exofacial double HA tag in domain II and mutations in the P loop of Domains I, II, III and IV which abolish trafficking to the cell surface
    Depositor
    Insert
    cacna1b (CACNA1B Rabbit)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    E314A, E664A, E1370A, E1658A
    Promoter
    CMV
    Available since
    Nov. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
  3. Cav2.2 HA EI,II,III,IVA (E314A, E664A, E1370A, E1658A) pCAGGS

    Plasmid
    #206098
    Purpose
    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and mutations in the P loop of Domains I, II, III and IV which abolish trafficking to the cell surface
    Depositor
    Insert
    cacna1b (CACNA1B Rabbit)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    E314A, E664A, E1370A, E1658A
    Promoter
    CMV/B-actin
    Available since
    Nov. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
  4. pX330.puro

    Plasmid
    #110403
    Purpose
    CRISPR/Cas9 vector with sgRNA expression and puromycin resistance
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
  5. pCAG-BE4max-P2A-EGFP (pRZ33)

    Plasmid
    #140083
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with P2A-EGFP
    Depositor
    Insert
    Human codon optimized pCAG BE4max with P2A-EGFP
    Use
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A (SpCas9)
    Promoter
    CAG
    Available since
    March 2, 2021
    Availability
    Academic Institutions and Nonprofits only
  6. AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato

    Plasmid
    #51083
    Purpose
    Cre dependent expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophore
    Depositor
    Has Service
    AAV Retrograde
    Insert
    GCaMP6f
    Use
    AAV
    Tags
    P2A-nls-dTomato
    Expression
    Mammalian
    Mutation
    Promoter
    hEF1a
    Available since
    Feb. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
  7. pCMV-ABE-P48R-UGI

    Plasmid
    #161817
    Purpose
    Expresses ABE-P48R-UGI in mammalian cells
    Depositor
    Insert
    bpNLS-TadA7.10(P48R)-32AA linker-hSpCas9n(D10A)-10AA linker-UGI-10AA linker-UGI-bpNLS-P2A-EGFP-NLS
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    D10A in S. pyogenes Cas9, TadA mutations describe…
    Promoter
    Available since
    Jan. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
  8. AAV-hSyn1-GCaMP6f-P2A-nls-dTomato

    Plasmid
    #51085
    Purpose
    Neuronal expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophore
    Depositor
    Has Service
    AAV Retrograde and AAV1
    Insert
    GCaMP6f
    Use
    AAV
    Tags
    P2A-nls-dTomato
    Expression
    Mammalian
    Mutation
    Promoter
    human Synapsin1
    Available since
    Feb. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
  9. AAV-hSyn1-GCaMP6s-P2A-nls-dTomato

    Plasmid
    #51084
    Purpose
    Neuronal expression of GCaMP6s Ca sensor and physically separate nuclear localized dTomato fluorophore
    Depositor
    Has Service
    AAV Retrograde and AAV1
    Insert
    GCaMP6s
    Use
    AAV
    Tags
    P2A-nls-dTomato
    Expression
    Mammalian
    Mutation
    Promoter
    human Synapsin1
    Available since
    Feb. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
  10. SOX9-T2A-NLS-tdTomato-PGK-Puro

    Plasmid
    #89993
    Purpose
    Donor template for generation of SOX9-ntdTomato reporter cell lines
    Depositor
    Insert
    SOX9-T2A-NLS-TdTomato-PGK-Puro (SOX9 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    none
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
  11. AAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato

    Plasmid
    #51082
    Purpose
    Cre dependent expression of GCaMP6s Ca sensor and physically separate nuclear localized dTomato fluorophore
    Depositor
    Has Service
    AAV1
    Insert
    GCaMP6s
    Use
    AAV
    Tags
    P2A-nls-dTomato
    Expression
    Mammalian
    Mutation
    Promoter
    hEF1a
    Available since
    Feb. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
  12. pEG302-SunTagng-22aa

    Plasmid
    #115345
    Purpose
    Encodes a SunTag CRISPR cas9 system that does not contain a guide RNA and therefore, does not target the TET1 catalytic domain to any specific region of the genome
    Depositor
    Insert
    NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Nov. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
  13. AAVS1_Puro_hPGK1_hGEM(1-110)_mNG_hSLBP(18-108)

    Plasmid
    #176932
    Purpose
    Donor for targeted integration of S-phase specific probe (FUCCI-S) to the AAVS1 safe harbor locus
    Depositor
    Insert
    hGEM(1-110)_mNG_hSLBP(18-108)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PGK1
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
  14. pJH2972

    Plasmid
    #100956
    Purpose
    Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. URA3MX marker
    Depositor
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Sept. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
  15. pJH2971

    Plasmid
    #100955
    Purpose
    Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. KANMX marker
    Depositor
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Sept. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
  16. pJH2970

    Plasmid
    #100954
    Purpose
    Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. HIS3MX marker
    Depositor
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Sept. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
  17. SOX17-tdTomato-EhB

    Plasmid
    #210467
    Purpose
    Donor plasmid for knock-in tdTomato into the human SOX17 locus
    Depositor
    Insert
    SOX17 homologous recombination arms with a NLS-tdTomato and EF1alpha-drived hygromycin-NLS-mTagBFP2 selection cassette (SOX17 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
  18. pYFP-NEAT1pr_v1-RT

    Plasmid
    #97087
    Purpose
    Encodes a HR repair template for knock-in a YFP expression cassette at the promoter of human NEAT1 gene. Best used with px330-NEAT1pr_v1 vector, but also works with px330-NEAT1pr_v2.
    Depositor
    Insert
    nuclear paraspeckle assembly transcript 1 (NEAT1 Human)
    Use
    CRISPR
    Tags
    EYFP
    Expression
    Mutation
    Promoter
    CMV
    Available since
    July 28, 2017
    Availability
    Academic Institutions and Nonprofits only
  19. pTFAM.1.0-gDNA

    Plasmid
    #132443
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Insert
    TFAM (TFAM Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
  20. pREST-donor

    Plasmid
    #113807
    Purpose
    CRISPR donor plasmid to tag human transcription factor REST with GFP
    Depositor
    Insert
    REST homology arms flanking EGFP-IRES-Neo cassette (REST Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    rs4864608
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
  21. pFETCh_ATF4

    Plasmid
    #72350
    Purpose
    Donor vector for 3' FLAG tag of human ATF4
    Insert
    ATF4 Homology Arms (ATF4 Human)
    Use
    CRISPR
    Tags
    3XFLAG-P2A-NeoR
    Expression
    Mutation
    Promoter
    Available since
    May 24, 2016
    Availability
    Academic Institutions and Nonprofits only
  22. px330_SMAD exon 9 gRNA

    Plasmid
    #68352
    Purpose
    px330 with gRNA towards SMAD exon 9. Cas9 is expressed from a CAG promoter.
    Insert
    SMAD exon 9 gRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
  23. pGRHL2.1.0-gDNA

    Plasmid
    #175863
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Insert
    GRHL2 (GRHL2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
  24. pTEAD1-donor

    Plasmid
    #112329
    Purpose
    CRISPR donor plasmid to tag human transcription factor TEAD1 with GFP
    Depositor
    Insert
    TEAD1 homology arms flanking EGFP-IRES-Neo cassette (TEAD1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
  25. pZBTB12.1.0-gDNA

    Plasmid
    #113769
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZBTB12
    Depositor
    Insert
    ZBTB12 (ZBTB12 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
  26. pZBTB17.1.0-gDNA

    Plasmid
    #112483
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZBTB17
    Depositor
    Insert
    ZBTB17 (ZBTB17 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 10751 - 10800 of 33610 results