We narrowed to 10,792 results for: yeast
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Plasmid#199763PurposeElongation reporter construct to quantify elongation of LacZDepositorInsertLacZ-nLucPEST
ExpressionYeastPromoterTetO7Available sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT Compatible pAIDA2(6FLAG)
Plasmid#193321PurposeC-SWAT compatible vector for C-terminal tagging with TAP-AID*-6FLAG. osTIR1 is carried downstream of the C-terminal tag.DepositorInsertTAP-AID*-6FLAG
TagsTAP-AID*-6FLAGExpressionYeastAvailable sinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHpgRNA50
Plasmid#160128PurposeTo express a sgRNA with constant targeting LSC2 by RNA pol II promoter PTEF1DepositorInsertPTEF1-HH-gRNA-LSC2- HDV-TAMO
ExpressionYeastAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMM2_12
Plasmid#183061PurposeControl for biosensor in pMM2_9: BM3R1-HAA1-mTurquoise2 under HAA1p and mCherry under HREsYGP1p with flanks for genome integration into HO locusDepositorInsertsmCherry
BM3R1-HAA1-mTurquoise2
UseSynthetic BiologyTagsmCherry and mTurquoise2ExpressionYeastPromoterHAA1 and Synthetic promoterAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMM2_10
Plasmid#183059PurposeControl for biosensor in pMM2_9: HAA1-mTurquoise2 under HAA1p and mCherry under HREsYGP1p with flanks for genome integration into HO locusDepositorInsertsmCherry
HAA1-mTurquoise2
UseSynthetic BiologyTagsmCherry and mTurquoise2ExpressionYeastPromoterHAA1 and Synthetic promoterAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMM2_9
Plasmid#183058PurposeBiosensor for acetic acid: BM3R1-Haa1-mTurquoise2 under HAA1p and mCherry under BM3R1ENO1cp with flanks for genome integration in toHO locusDepositorInsertsmCherry
BM3R1-HAA1-mTurquoise2
UseSynthetic BiologyTagsmCherry and mTurquoise2ExpressionYeastPromoterHAA1 and Synthetic promoterAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMM2_11
Plasmid#183060PurposeControl for biosensor in pMM2_9: HAA1-mTurquoise2 under HAA1p and mCherry under HREsENO1cp with flanks for genome integration into HO locusDepositorInsertsmCherry
HAA1-mTurquoise2
UseSynthetic BiologyTagsmCherry and mTurquoise2ExpressionYeastPromoterHAA1 and Synthetic promoterAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJAID5XY
Plasmid#165051PurposeIntegrative expression of mRFP-CUP1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.DepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1>mRFP-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
ExpressionYeastMutationOsTIR1 is codon-optimised for expression in S. ce…Available sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJAID5VY
Plasmid#165048PurposeIntegrative expression of CUP1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.DepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1>CUP1-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
ExpressionYeastMutationOsTIR1 is codon-optimised for expression in S. ce…Available sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJAID6A
Plasmid#165046PurposeIntegrative expression of yEGFP and rice auxin receptor gene OsTIR1. OsTIR1 is codon-optimised for expression in S. cerevisiaeDepositorInsertP-URA3>KlURA3>TAgTEF1-P-TEF1>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
ExpressionYeastMutationWTAvailable sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
A102 ΔAmpH Δ2x+ve
Plasmid#129484PurposeExpression of Glo3 ΔAmpH lacking the COPI-binding motif in E.ColiDepositorInsertADP-ribosylation factor GTPase-activating protein (GLO3 Budding Yeast)
TagsHis and MBPExpressionBacterialAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
A102-GAP-BoCCS-GRM
Plasmid#123286PurposeExpression of Glo3 ΔAmpH (1-459) in E.ColiDepositorInsertADP-ribosylation factor GTPase-activating protein (GLO3 Budding Yeast)
TagsHis and MBPExpressionBacterialPromoterT5Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT45_GalL_nCDA1-BE3
Plasmid#145038PurposeExpresses nCDA1-BE3 in yeast cellsDepositorInsertnCDA1-BE3
UseCRISPRExpressionYeastMutationspCas9(D10A)PromoterGalLAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT166_GalL_A3A-BE3
Plasmid#145116PurposeExpressing base editor A3A-BE3 in yeast cellsDepositorInsertA3A-BE3
UseCRISPRExpressionYeastMutationspCas9(D10A)PromoterGalLAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT131_GalL_cCDA1-NGBE3
Plasmid#145095PurposeExpressing base editor cCDA1-NGBE3 in yeast cellsDepositorInsertcCDA1-NGBE3
UseCRISPRExpressionYeastMutationspCas9(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1…PromoterGalLAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT130_GalL_nCDA1-NGBE3
Plasmid#145094PurposeExpressing base editor nCDA1-NGBE3 in yeast cellsDepositorInsertnCDA1-NGBE3
UseCRISPRExpressionYeastMutationspCas9(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1…PromoterGalLAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT121_GalL_nCDA1-VQRBE3
Plasmid#145086PurposeExpressing base editor nCDA1-VQRBE3 in yeast cellsDepositorInsertnCDA1-VQRBE3
UseCRISPRExpressionYeastMutationspCas9(D10A, D1135V, R1335Q, T1337R)PromoterGalLAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT79_GalL_nCDA1Δ192-BE3
Plasmid#145045PurposeExpresses nCDA1Δ192-BE3 in yeast cellsDepositorInsertnCDA1Δ192-BE3
UseCRISPRExpressionYeastMutationpmCDA1(1-192AA); spCas9(D10A)PromoterGalLAvailable sinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAV0816
Plasmid#133510PurposeExpression of mTagBFP2 tagged CRIB domain to monitor active Cdc42DepositorInsertpAde6(PmeI)-p(act1)-CRIB(gic2aa2-181)-mTagBFP2-terminator(ScADH1)-bsdMX
ExpressionYeastAvailable sinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCfB8382
Plasmid#126916PurposePlasmid containing integration cassette at XI-3 integration site in Saccharomyces cerevisiae for overexpression of XdCrtIDepositorInsertXdCrtI
ExpressionYeastAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only