We narrowed to 10,119 results for: yeast

1. P1K-3HA-Atg8∆R-MET15

   Plasmid

   #207034

   Purpose

   Expression of 3HA-Atg8∆R. This variant bypasses the need of Atg4 for initial C-terminal processing.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   ATG8
   

   Tags

   3xHA

   Expression

   Yeast

   Mutation

   ∆R

   Promoter

   pATG8

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. YL-CashGem-BAT1-URA3-LINEAR

   Plasmid

   #174837

   Purpose

   pRS414 backbone containing LINEAR fragment targeting BAT1 in Y. lipolytica

   Depositor

   Zengyi Shao

   Article

   Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

   Insert

   Codon optimized Cas9:hGem
   

   Use

   CRISPR and Synthetic Biology

   Tags

   hGeminin tag

   Expression

   Bacterial and Yeast

   Promoter

   GPDp

   Available Since

   Jan. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTDH3-dCas9-Mxi1

   Plasmid

   #46921

   Purpose

   Yeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS and Mxi1 domain controlled by TDH3 promoter

   Depositor

   Stanley Qi, Jonathan Weissman

   Article

   Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

   Insert

   dCas9-Mxi1
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   TDH3

   Available Since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pYES2/103Q

   Plasmid

   #1385

   Depositor

   Michael Sherman

   Article

   Meriin et al J Cell Biol 2002 Jun 10;157(6):997-1004.

   Insert

   huntingtin (103Q) (HTT Human)
   

   Tags

   EGFP and FLAG

   Expression

   Yeast

   Mutation

   Exon1 sequences containing the first 17 amino aci…

   Available Since

   Feb. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. BS-TRP1-TetOff(7repeats)-GFP-Pho8

   Plasmid

   #207011

   Purpose

   Tet-Off controlled GFP-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   Pho8
   

   Tags

   GFP

   Expression

   Yeast

   Available Since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRR-AR

   Plasmid

   #73045

   Purpose

   Constitutively expresses the androgen receptor, which is required for the activation of the promoter in pLAREG (Addgene plasmid #73045)

   Depositor

   David McMillen

   Article

   Mazumder et al Nucleic Acids Res. 2014 Aug;42(14):9514-

   Insert

   Androgen receptor (AR Human)
   

   Expression

   Yeast

   Mutation

   please see depositor comments below

   Promoter

   glyceraldehyde phosphate dehydrogenase (GPD)

   Available Since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pYES2/25Q

   Plasmid

   #1384

   Depositor

   Michael Sherman

   Article

   Meriin et al J Cell Biol 2002 Jun 10;157(6):997-1004.

   Insert

   huntingtin (25Q) (HTT Human)
   

   Tags

   EGFP and FLAG

   Expression

   Yeast

   Mutation

   Exon1 sequences containing the first 17 amino aci…

   Available Since

   Feb. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFA6a-kanMX6-P41nmt1-GFP

   Plasmid

   #39290

   Depositor

   Jurg Bahler, John Pringle

   Article

   Bahler et al Yeast. 1998 Jul;14(10):943-51.

   Insert

   nmt1 promoter (nmt1 Fission Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   A. gossypii translation elongation factor 1a gene…

   Mutation

   -1163 to +6 of nmt1 locus with a 4bp deletion (TA…

   Available Since

   Aug. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pFA6a-kanMX6-P81nmt1-GFP

   Plasmid

   #39291

   Depositor

   Jurg Bahler, John Pringle

   Article

   Bahler et al Yeast. 1998 Jul;14(10):943-51.

   Insert

   nmt1 promoter (nmt1 Fission Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   A. gossypii translation elongation factor 1a gene…

   Mutation

   -1163 to +6 of nmt1 locus with a 7bp deletion (AT…

   Available Since

   Aug. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

10. PADH1-TIR1-9myc-URA3

    Plasmid

    #207016

    Purpose

    Expresses TIR1-9Myc. TIR1 mediates auxin-induced ubiquitination of auxin-inducible degron (AID).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    TIR1
    

    Tags

    9xMyc

    Expression

    Yeast

    Promoter

    pADH1

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRR-ERbeta-5Z

    Plasmid

    #23062

    Depositor

    Charles Miller

    Article

    Miller CA et al Plasmid. 2010 Mar;63(2):73-8. doi: 10.10

    Insert

    ERbeta (ESR2 Human)
    

    Expression

    Yeast

    Available Since

    Jan. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pMT957

    Plasmid

    #228293

    Purpose

    Constitutive mutant PhlTA expression with strong promoter

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

    Insert

    phlTA mutant
    

    Use

    Synthetic Biology

    Tags

    Three copies of VP16 (VP48)-Nuclear localization …

    Expression

    Yeast

    Mutation

    E41G

    Promoter

    ScPGK1 promoter

    Available Since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMT1035

    Plasmid

    #228300

    Purpose

    Constitutive expression of LuxTA mutant with strong promoter

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

    Insert

    luxTA mutant
    

    Use

    Synthetic Biology

    Tags

    Nuclear localization signal-Three copies of VP16 …

    Expression

    Yeast

    Mutation

    S116Y, W201R

    Promoter

    KpGAPDH promoter

    Available Since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCashGem-Xyl2-URA3-LINEAR

    Plasmid

    #174840

    Purpose

    pRS414 backbone containing LINEAR fragment targeting XYL2 in S. stipitis

    Depositor

    Zengyi Shao

    Article

    Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

    Insert

    Codon optimized Cas9:hGem
    

    Use

    CRISPR and Synthetic Biology

    Tags

    hGeminin tag

    Expression

    Bacterial and Yeast

    Promoter

    ENO1p

    Available Since

    Jan. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pUDC175

    Plasmid

    #103019

    Purpose

    S. cerevisae centromic plasmid harboring Fncpf1 under control of TEF1 promoter

    Depositor

    Jean-Marc Daran

    Article

    Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

    Insert

    Fncpf1
    

    Tags

    3HA and NLS

    Expression

    Yeast

    Mutation

    human codon optimized

    Promoter

    TEF1

    Available Since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pESC-URA::CYP71AV1/CPR

    Plasmid

    #20118

    Depositor

    Jay Keasling

    Article

    Ro et al Nature. 2006 Apr 13. 440(7086):940-3.

    Insert

    amorphadiene oxidase and p450 reductase
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Available Since

    March 2, 2009

    Availability

    Academic Institutions and Nonprofits only

17. pRR-AR-5Z

    Plasmid

    #23058

    Depositor

    Charles Miller

    Article

    Miller CA et al Plasmid. 2010 Mar;63(2):73-8. doi: 10.10

    Insert

    AR (AR Human)
    

    Expression

    Yeast

    Available Since

    April 29, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pFA6a-kanMX6-P41nmt-3HA

    Plasmid

    #39284

    Depositor

    Jurg Bahler, John Pringle

    Article

    Bahler et al Yeast. 1998 Jul;14(10):943-51.

    Insert

    nmt1 promoter (nmt1 Fission Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    3xHA, A. gossypii translation elongation factor 1…

    Mutation

    -1163 to +6 of nmt1 locus with a 4bp deletion (TA…

    Available Since

    Aug. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pFA6a-kanMX6-P81nmt1-3HA

    Plasmid

    #39285

    Depositor

    Jurg Bahler, John Pringle

    Article

    Bahler et al Yeast. 1998 Jul;14(10):943-51.

    Insert

    nmt1 promoter (nmt1 Fission Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    3xHA, A. gossypii translation elongation factor 1…

    Mutation

    -1163 to +6 of nmt1 locus with a 7bp deletion (AT…

    Available Since

    Aug. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pAG32-3HA-3FLAG

    Plasmid

    #154081

    Purpose

    For C-terminal 3xHA-3xFLAG-protein tagging in S. pombe using hphMX4 as resistance marker.

    Depositor

    Cornelia Kilchert

    Article

    Kilchert et al Genome Res. 2020 Jul;30(7):1012-1026. do

    Insert

    3xHA-3xFLAG-hygR
    

    Use

    Pcr-based tagging in yeast

    Tags

    3xHA-3xFLAG

    Available Since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge