We narrowed to 159,352 results for: addgene

1. hU6-DR_BsmBI_DR-EFS-RfxCas13d-NLS-2A-Puro-WPRE CasRx pre-gRNA backbone

   Plasmid

   #219823

   Purpose

   EF1α-driven expression of RfxCas13d in mammalian cells. For cloning of pre-guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. (Adapted from plasmid #138147)

   Depositor

   Philipp Maass

   Insert

   RfxCas13d
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a core promoter

   Available since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSCL391

   Plasmid

   #219501

   Purpose

   Express Ade2 and Faa1 editrons from a single Gal7 promoter, separated by a Csy4 recognition sequence

   Depositor

   Seth Shipman

   Article

   Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.

   Insert

   Eco1 editing ncRNAs and Cas9 gRNAs to edit ADE2 and FAA1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL7

   Available since

   May 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. LEGO-pDHS-AP1x6-GM55

   Plasmid

   #217416

   Purpose

   Luciferase expression vector with 6 AP-1 sites and the minimal GM-CSF promoter, and a chromatin priming element. Alias: LEGO-GM55-AP-1x6-pDHS(IL-3)

   Depositor

   Peter Cockerill

   Insert

   Luciferase, GFP
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   6 AP-1 sites, Human -55 to +28 minimal CSF2 promo…

   Available since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. LEGO-pDHS-ITDE-AP1x6-DUSP5

   Plasmid

   #217419

   Purpose

   Luciferase expression vector with an oncogene-inducible enhancer, 6 AP-1 sites, the full length DUSP5 promoter, and a chromatin priming element. Alias: LEGO-DUSP5p-AP-1x6-ITDe

   Depositor

   Peter Cockerill

   Insert

   Luciferase, GFP
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human DUSP5 promoter

   Available since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. SHLD3 N-terminal sgRNA

   Plasmid

   #207094

   Purpose

   pX330 based plasmid for expression of Cas9 and the TTACTGCAGAATGACTACAG sgRNA to target the SHLD3 locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   TTACTGCAGAATGACTACAG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. NBS1 C-terminal sgRNA

   Plasmid

   #207092

   Purpose

   pX330 based plasmid for expression of Cas9 and the TAAAAAGGAGAAGATAACTG sgRNA to target the NBS1 locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   TAAAAAGGAGAAGATAACTG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. RNF169 C-terminal sgRNA

   Plasmid

   #207099

   Purpose

   pX330 based plasmid for expression of Cas9 and the ACACTTCATTAGGTGCTACT sgRNA to target the RNF169 locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   ACACTTCATTAGGTGCTACT
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. SHLD1 N-terminal sgRNA

   Plasmid

   #207101

   Purpose

   pX330 based plasmid for expression of Cas9 and the ATGGCAGGACTATGGCAGCC sgRNA to target the SHLD1 locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   ATGGCAGGACTATGGCAGCC
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. ATM C-terminal sgRNA

   Plasmid

   #207097

   Purpose

   pX330 based plasmid for expression of Cas9 and the TTTCTAAAGGCTGAATGAAA sgRNA to target the ATM locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   TTTCTAAAGGCTGAATGAAA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. GOLIM4 g2 LentiCRISPRv2-mCherry

    Plasmid

    #218663

    Purpose

    Knockout vector for human GOLIM4 (GPP130/GOLPH4)

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    GOLIM4 (GOLIM4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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