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Plasmid#228487Purpose10xHis-SUMO (Smt3) fused, HRV-3C cleavable, GST-tag for rapid cloning/DNA assembly of pulldown targets via NotI restriction site for E. coli based protein expressionDepositorTypeEmpty backboneUseTags10xHis-SUMO(Smt3)-GST-HRVExpressionBacterialMutationPromoterT7 PromoterAvailable sinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only
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SUMO-PNGaseF
Plasmid#228498Purpose10xHis-SUMO (Smt3) tagged PNGaseF (E. coli based protein expression)DepositorInsertngl
UseTags10xHis-SUMO(Smt3)ExpressionBacterialMutationPromoterT7 PromoterAvailable sinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
MZB211
Plasmid#228494Purpose10xHis-SUMO (Smt3) tagged Ssod7-fused Pfu Polymerase with fidelity enhancing mutation (E. coli based protein expression)DepositorInsertpol
UseTags10xHis-SUMO(Smt3)ExpressionBacterialMutationA408SPromoterT7 PromoterAvailable sinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-21a(+)/MBP-His
Plasmid#38006DepositorInsertMBP (Maltose binding protein)
UseTags6xHISExpressionBacterialMutationPromoterAvailable sinceOct. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJA5 H-2K(b)
Plasmid#180441PurposeE. coli expression of soluble MHC proteinDepositorInsertH-2K(b)
UseTagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…PromoterAvailable sinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTCF120 H-2D(b)
Plasmid#180454PurposeE. coli expression of soluble MHC proteinDepositorInsertH-2D(b)
UseTagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…PromoterAvailable sinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
SMS
Plasmid#25234PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertSMS_01
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTCF3 H-2D(d)
Plasmid#180452PurposeE. coli expression of soluble MHC proteinDepositorInsertH-2D(d)
UseTagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…PromoterAvailable sinceFeb. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTCF170 H-2L(d)
Plasmid#180458PurposeE. coli expression of soluble MHC proteinDepositorInsertH-2L(d)
UseTagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…PromoterAvailable sinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -