We narrowed to 6,994 results for: ef1a

1. pSBbi-moxGFP-P2A-T2A-cMyc-Puro

   Plasmid

   #176892

   Purpose

   Sleeping Beauty transposon-based vector for mammalian polycistronic expression of moxGFP, c-Myc and an additional gene of interest

   Depositor

   Jorge Luis Arias-Arias

   Article

   Acon et al iScience. 2021 Nov 8;24(12):103407. doi:

   Insert

   moxGFP-P2A-T2A-c-Myc
   

   Use

   Transposon

   Tags

   Myc and moxGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a/RPBSA

   Available since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLEX307-mFzd9

   Plasmid

   #102877

   Purpose

   Expresses mouse Fzd9 in mammalian cells

   Depositor

   Henry Ho

   Article

   Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

   Insert

   Frizzled 9 (Fzd9 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   A to G substitution at nucleotide 114, G to A sub…

   Promoter

   EF1A

   Available since

   Dec. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-EF1α1.1-FLPX-rc [Chronos-tdTomato]

   Plasmid

   #122103

   Purpose

   AAV-mediated expression of Chronos-tdTomato under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chronos-tdTomato
   

   Use

   AAV

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a（1.1kb short version)

   Available since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPHAGE-EF1α-Luc-C20orf24-3’UTR-WT

   Plasmid

   #128551

   Purpose

   Lentiviral constitutive expression of Firefly luciferase under control of WT 3'UTR of human C20orf24.

   Depositor

   Mohan Babu

   Article

   Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLX307/PTPN11 T73I

   Plasmid

   #140939

   Purpose

   expresses PTPN11 T73I

   Depositor

   Kimberly Stegmaier

   Article

   Cremer et al Cancer Discov. 2020 Feb;10(2):214-231. d

   Insert

   PTPN11 protein tyrosine phosphatase non-receptor type 11 [ Homo sapiens (human) ] (PTPN11 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   T73I

   Promoter

   EF1a

   Available since

   June 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLX307/PTPN11 E76G

   Plasmid

   #140862

   Purpose

   expresses PTPN11 E76G

   Depositor

   Kimberly Stegmaier

   Article

   Cremer et al Cancer Discov. 2020 Feb;10(2):214-231. d

   Insert

   PTPN11 protein tyrosine phosphatase non-receptor type 11 [ Homo sapiens (human) ] (PTPN11 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E76G

   Promoter

   EF1a

   Available since

   June 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pB-TR-hCAII

   Plasmid

   #232480

   Purpose

   Tetracycline inducible PiggyBac vector expressing human CAII gene including flag-tag (DYKDDDDK) on the 3' end

   Depositor

   Lukas Trantirek

   Article

   Rynes et al Commun Biol. 2025 Feb 7;8(1):194. doi: 1

   Insert

   Human carbonic anhydrase II (CA2 Human)
   

   Use

   Transposon system

   Tags

   DYKDDDDK (FLAG-tag)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TetO

   Available since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. VEGF120TAG

   Plasmid

   #200946

   Purpose

   Encodes recombinant mouse VEGF120 when coupled with amber stop codon suppression by non-canonical amino acid incorporation

   Depositor

   Elizabeth Kirby

   Article

   Miller et al Mol Psychiatry. 2024 Nov 11. doi: 10.103

   Inserts

   VEGF120TAG (Vegfa Mouse)
   hPGK-driven Puromycin resistance
   

   Use

   Lentiviral

   Tags

   6x HIS

   Expression

   Mutation

   amber stop codon 101 bp from ATG

   Promoter

   Ef1a and hPGK

   Available since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. VEGF164TAG

   Plasmid

   #200947

   Purpose

   Encodes recombinant mouse VEGF164 when coupled with amber stop codon suppression by non-canonical amino acid incorporation

   Depositor

   Elizabeth Kirby

   Article

   Miller et al Mol Psychiatry. 2024 Nov 11. doi: 10.103

   Inserts

   VEGF164TAG (Vegfa Mouse)
   hPGK-driven Puromycin resistance
   

   Use

   Lentiviral

   Tags

   6x HIS

   Expression

   Mutation

   amber stop codon 101 bp from ATG

   Promoter

   Ef1a and hPGK

   Available since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFORF0452

    Plasmid

    #143879

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    THRA (THRA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. TFORF1357

    Plasmid

    #143101

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ILF3 (ILF3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. TFORF0453

    Plasmid

    #142628

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    THRA (THRA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pU6-ccdB-boxB-tBE-V5-mA3

    Plasmid

    #171693

    Purpose

    Expresses tBE-V5-mA3 in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

    Insert

    ccdB-sgRNA scaffold-boxB-tBE-V5-mA3
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and EF1a

    Available since

    Aug. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSBbi-Pur-Lyn-AktAR2-D

    Plasmid

    #125198

    Purpose

    SB-transposon plasmid for stable expression of unphosphorylatable "dead" version of lipid raft-targeted Lyn-AktAR2 variant of FRET-based AKT activity reporter

    Depositor

    Eric Davis

    Article

    Havranek et al Blood. 2017 Aug 24;130(8):995-1006. doi:

    Insert

    Lyn-AktAR2-D
    

    Use

    Transposon

    Tags

    Lyn tag (GCIKSKRKD), mCerulean3 and cpVenus[E172]

    Expression

    Mammalian

    Mutation

    FoxO1 Thr-24 changed to Val rendering this inacti…

    Promoter

    EF1a/RPBSA

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR223-DKFZp434B1231

    Plasmid

    #23508

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    DKFZp434B1231 (IGFN1 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    3MMs

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pPB-AMBRA1-3xFLAG

    Plasmid

    #174159

    Purpose

    PiggyBac vector to stably express 3xFLAG-tagged AMBRA1

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    AMBRA1 (AMBRA1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLV-hCCND1-HA

    Plasmid

    #174154

    Purpose

    Lentiviral vector expressing HA-tagged human cyclin D1

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Lentiviral

    Tags

    hemagglutinin (HA)

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLV-hCCND1-T2A-hCCND2-P2A-hCCND3

    Plasmid

    #174156

    Purpose

    Lentiviral vector expressing all three D-type cyclins (cyclin D1, cyclin D2, cyclin D3) from a single promoter

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    CCND1>T2A>CCND2>P2A>CCND3
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLEX307-mFzd8

    Plasmid

    #102870

    Purpose

    Expresses mouse Fzd8 in mammalian cells

    Depositor

    Henry Ho

    Article

    Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

    Insert

    Frizzled 8 (Fzd8 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    T to C substitution at nucleotide 1134, C to T su…

    Promoter

    EF1A

    Available since

    Jan. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pB-CuR-hSOD1

    Plasmid

    #232477

    Purpose

    The inducible PiggyBac Cumate Switch vector (PBQM812A-1 System Biosciences) expressing human SOD1gene including flag -tag (DYKDDDDK) on the 3' end

    Depositor

    Lukas Trantirek

    Article

    Rynes et al Commun Biol. 2025 Feb 7;8(1):194. doi: 1

    Insert

    Homo sapiens superoxide dismutase 1 (SOD1 Human)
    

    Use

    Transposon system

    Tags

    DYKDDDDK (FLAG-tag)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-CuO

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pB-TR-hSOD1

    Plasmid

    #232478

    Purpose

    Tetracycline inducible PiggyBac vector expressing human SOD1gene including flag-tag (DYKDDDDK) on the 3' end

    Depositor

    Lukas Trantirek

    Article

    Rynes et al Commun Biol. 2025 Feb 7;8(1):194. doi: 1

    Insert

    Homo sapiens superoxide dismutase 1 (SOD1 Human)
    

    Use

    Transposon system

    Tags

    DYKDDDDK (FLAG-tag)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSLCAR-CD19-28z

    Plasmid

    #135991

    Purpose

    Modular CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production

    Depositor

    Scott McComb

    Article

    Bloemberg et al Mol Ther Methods Clin Dev. 2020 Jan 31;1

    Insert

    FMC63-28-3z-P2A-EGFP (CD28, CD247 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a-short

    Available since

    June 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSLCAR-CD19-CD3z

    Plasmid

    #135993

    Purpose

    Modular CD3z alone CAR backbone, For Transient Expression or Lentiviral Production

    Depositor

    Scott McComb

    Article

    Bloemberg et al Mol Ther Methods Clin Dev. 2020 Jan 31;1

    Insert

    FMC63-3z-P2A-EGFP (CD247, CD28 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a-short

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-mTubb3

    Plasmid

    #87116

    Purpose

    AAV backbone plasmid including GFP knock-in donor and Tubb3gRNA for HITI

    Depositor

    Juan Belmonte

    Article

    Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

    Insert

    U6-mTubb3sgRNA-GFP-EF1a-mCherryKASH-hGHpA
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-Ai14-HITI

    Plasmid

    #87117

    Purpose

    AAV backbone plasmid including GFPNLS-pA knock-in donor and Ai14gRNA for HITI

    Depositor

    Juan Belmonte

    Article

    Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

    Insert

    U6-Ai14sgRNA-GFPNLS-EF1a-mCherryKASH-pA
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLV-hCCND1-T286A-HA

    Plasmid

    #174155

    Purpose

    Lentiviral vector expressing HA-tagged human cyclin D1 with T286A mutation

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Lentiviral

    Tags

    hemagglutinin (HA)

    Expression

    Mammalian

    Mutation

    A>G at base 856 (Thr>Ala at amino acid 286)

    Promoter

    EF1A

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pPB-AMBRA1-501*-3xFLAG

    Plasmid

    #174160

    Purpose

    PiggyBac vector to stably express 3xFLAG-tagged AMBRA1 truncated at amino acid 501

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    AMBRA1 (AMBRA1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Missing aa 501-1208

    Promoter

    EF1A

    Available since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pPB-AMBRA1-217*-3xFLAG

    Plasmid

    #174161

    Purpose

    PiggyBac vector to stably express 3xFLAG-tagged AMBRA1 truncated at amino acid 217

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    AMBRA1 (AMBRA1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Missing aa 217-1208

    Promoter

    EF1A

    Available since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-EF1α1.1-FLEX-rc [Archon1-KGC-GFP-ER2]

    Plasmid

    #115891

    Purpose

    AAV-mediated expression of Archon1-KGC-GFP-ER2 under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    Archon1-KGC-GFP-ER2
    

    Use

    AAV

    Tags

    ER2, GFP, and KGC

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α promoter (1.1kb short version)

    Available since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMaster1

    Plasmid

    #58525

    Purpose

    Reprogram somatic cells to iPS cells

    Depositor

    Mario Capecchi

    Article

    Wu et al Proc Natl Acad Sci U S A. 2014 Jul 22;11

    Inserts

    neo
    tk
    oct4 (POU5F1 Human)
    Klf4 (KLF4 Human)
    Sox2 (SOX2 )
    myc (MYC Human)
    EBNA-1
    Amp
    Lin28 (LIN28A Human)
    Nanog (NANOG Human)
    IRES
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    CAG and EF1a

    Available since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge