We narrowed to 2,297 results for: pet28

1. mEGFP-NbALFA

   Plasmid

   #159986

   Purpose

   For the expresson and purification of the EGFP-tagged anti-ALFA tag nanobody (NbALFA)

   Depositor

   Motoyuki Hattori

   Article

   Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

   Insert

   mEGFP-NbALFA
   

   Use

   Tags

   8histidine-HRV3C protease cleavage site

   Expression

   Bacterial

   Mutation

   3GS linker between mEGFP and NbALFA

   Promoter

   Available since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-28a-His6-SUMO-Munc18-1wt

   Plasmid

   #135550

   Purpose

   Express rat Munc18-1 in E. coli BL21, purified via HisPur Ni-NTA Resin

   Depositor

   Jingshi Shen

   Article

   Yu et al Methods Mol Biol. 2019;1860:237-249. doi

   Insert

   Munc18-1wt
   

   Use

   Tags

   6xHis tag, thrombin recognition and cleavage site…

   Expression

   Bacterial

   Mutation

   No ATG start codon

   Promoter

   T7 promoter

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. PET-28a-YTH domain of YTHDF3

   Plasmid

   #228777

   Purpose

   Protein expression plasmid for YTH domain of YTHDF3 in E.Coli.

   Depositor

   Huiqing Zhou

   Article

   Wang et al RSC Chem Biol. 2024 Jul 25;5(9):914-923.

   Insert

   YTH domain of YTHDF3 (YTHDF3 Human)
   

   Use

   Tags

   6*His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. PET-28a-YTH domain of YTHDF2

   Plasmid

   #228776

   Purpose

   Protein expression plasmid for YTH domain of YTHDF2 in E.Coli.

   Depositor

   Huiqing Zhou

   Article

   Wang et al RSC Chem Biol. 2024 Jul 25;5(9):914-923.

   Insert

   YTH domain of YTHDF2 (YTHDF2 Human)
   

   Use

   Tags

   6*His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-CjCas9

   Plasmid

   #89754

   Purpose

   Expression of CjCas9 with His tag in E.coli

   Depositor

   Seokjoong Kim

   Article

   Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

   Insert

   CjCas9
   

   Use

   CRISPR

   Tags

   6XHis, HA, and SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. AviTag-SpyTag003-MBP

   Plasmid

   #184227

   Purpose

   Expresses AviTag and SpyTag003 linked to maltose binding protein in the bacterial cytoplasm. AviTag enables site-specific biotinylation by BirA. SpyTag003 enables reaction with SpyCatcher003.

   Depositor

   Mark Howarth

   Article

   Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

   Insert

   AviTag-SpyTag003-MBP
   

   Use

   Tags

   AviTag, His6, SpyTag003, and Thrombin cleavage si…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Kindlin

   Plasmid

   #186135

   Purpose

   For expression of recombinant kindlin in bacteria

   Depositor

   Michael Rosen

   Article

   Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

   Insert

   FERMT2 (FERMT2 Human)
   

   Use

   Tags

   His6-sumo

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT7-HISx6-SIMx6

   Plasmid

   #116938

   Purpose

   Bacterial expression vector for 6 sim

   Depositor

   Liam Holt

   Article

   Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

   Insert

   6 sim
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT7-HISx6-SUMOx10

   Plasmid

   #116937

   Purpose

   Bacterial expression vector for 10 sumo

   Depositor

   Liam Holt

   Article

   Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

   Insert

   10 SUMO
   

   Use

   Tags

   HIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pIA1363

    Plasmid

    #166861

    Purpose

    Expression of codon optimized SARS-COV-2 Nsp8 in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

    Insert

    SARS-COV-2 Nsp8 (ORF1ab Synthetic)
    

    Use

    Tags

    His-GB1

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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