We narrowed to 6,994 results for: ef1a

1. pSLCAR-CD19-BBz

   Plasmid

   #135992

   Purpose

   Modular 41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

   Depositor

   Scott McComb

   Article

   Bloemberg et al Mol Ther Methods Clin Dev. 2020 Jan 31;1

   Insert

   FMC63-41BB-3z-P2A-EGFP (CD247, CD28, TNFRSF9 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a-short

   Available since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. BII-ChPtW-tdT

   Plasmid

   #133401

   Purpose

   Piggybac vector for constitutive expression of tdTomato

   Depositor

   Blair Madison

   Insert

   tdTomato
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CMV enhancer and hEf1a (CpG free)

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. BII-ChPtW-iRFP702

   Plasmid

   #133398

   Purpose

   Piggybac vector for constitutive expression of iRFP702

   Depositor

   Blair Madison

   Insert

   IRFP-702
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CMV enhancer and hEf1a (CpG free)

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. BII-ChPtW-iRFP670

   Plasmid

   #133397

   Purpose

   Piggybac vector for constitutive expression of iRFP670-NLS

   Depositor

   Blair Madison

   Insert

   iRFP-670-NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CMV enhancer and hEf1a (CpG free)

   Available since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. BII-ChPtW-smURFP

   Plasmid

   #133399

   Purpose

   Piggybac vector for constitutive expression of smURFP-NLS

   Depositor

   Blair Madison

   Insert

   smURFP-NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CMV enhancer and hEf1a (CpG free)

   Available since

   Jan. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. BII-Sh-BnTW

   Plasmid

   #133365

   Purpose

   Piggybac vector for shRNA-mediated knockdown, with co-expression with tdTomato-NLS

   Depositor

   Blair Madison

   Insert

   tdTomato-NLS
   

   Use

   Tags

   Flag-tagged tdTomato

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CMV enhancer and hEf1a (CpG free)

   Available since

   Jan. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSBbi-pur H-2Kb

   Plasmid

   #111623

   Purpose

   Sleeping Beauty transposon vector encoding H-2Kb mouse MHC Class I allele

   Depositor

   Jon Yewdell

   Insert

   H2Kb
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   June 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. BII-gR-PnTW

   Plasmid

   #133356

   Purpose

   Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications) coexpressed with tdTomato-NLS

   Depositor

   Blair Madison

   Insert

   tdTomato-NLS
   

   Use

   Tags

   HA-tagged tdTomato

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CMV enhancer and hEf1a (CpG free)

   Available since

   Jan. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-double floxed-eNpHR-EYFP-WPRE-pA

   Plasmid

   #20949

   Purpose

   Cre-activated AAV expression of halorhodopsin 2.0 (eNpHR) fused to EYFP for optogenetic inhibition

   Depositor

   Karl Deisseroth

   Has Service

   AAV9

   Insert

   halorhodopsin
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEF1-MH.Bl-mDcrSOM

    Plasmid

    #120540

    Purpose

    protein expression

    Depositor

    Petr Svoboda

    Insert

    Dicer (Dicer1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEF1-MH.Bl-mDcrOO

    Plasmid

    #120541

    Purpose

    protein expression

    Depositor

    Petr Svoboda

    Insert

    Dicer (Dicer1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. p16L1-GFP

    Plasmid

    #89910

    Purpose

    Self-packages in TT-16L2 stable line. L1-only capsid propagation.

    Depositor

    John Schiller

    Article

    Buck et al J Virol. 2005 Mar;79(5):2839-46.

    Insert

    HPV16L1+ GFP
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    SV40 and hEF1alpha

    Available since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCS4077

    Plasmid

    #131738

    Purpose

    pLenti_CMV-mCherry-BghA_EF1alpha-BFP

    Depositor

    Christina Smolke

    Article

    Xiang et al Nat Commun. 2019 Sep 23;10(1):4327. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. p2151 pHAGE-hSPC-TFEB-TagBFP-W

    Plasmid

    #188722

    Purpose

    Lentiviral vector allowing for SFTPC-driven expression of TFEB:tagBFP fusion protein

    Depositor

    Darrell Kotton

    Insert

    TFEB (TFEB Human)
    

    Use

    Lentiviral

    Tags

    tagBFP

    Expression

    Mutation

    Promoter

    hSP-C

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. Aiolos ZF2 (AA146-168) in Cilantro 2

    Plasmid

    #74456

    Purpose

    Monitor post-translational degradation of Aiolos ZF2 via EGFP:mCherry ratio (Lentiviral, PGK.AiolosAA146-168-10AAFlexLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR)

    Depositor

    Benjamin Ebert

    Insert

    Aiolos (IKZF3) AA130-189 (IKZF3 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. SOX17-tdTomato-EhB

    Plasmid

    #210467

    Purpose

    Donor plasmid for knock-in tdTomato into the human SOX17 locus

    Depositor

    Kai Wang

    Insert

    SOX17 homologous recombination arms with a NLS-tdTomato and EF1alpha-drived hygromycin-NLS-mTagBFP2 selection cassette (SOX17 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJA317

    Plasmid

    #74488

    Purpose

    eF1alpha::puroR::mCherry::T2A::eGFP::d1ODC::wPRE has d1ODC degron

    Depositor

    Andrew Fire

    Article

    Arribere et al Nature. 2016 Jun 1;534(7609):719-723. do

    Insert

    d1ODC
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJA318

    Plasmid

    #74489

    Purpose

    eF1alpha::puroR::mCherry::T2A::eGFP::d4ODC::wPRE has d4ODC degron

    Depositor

    Andrew Fire

    Article

    Arribere et al Nature. 2016 Jun 1;534(7609):719-723. do

    Insert

    d4ODC
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. EdTP

    Plasmid

    #24311

    Depositor

    Roel Nusse

    Article

    Fuerer et al PLoS One. 2010 . 5(2):e9370.

    Insert

    EF1alpha-dnhTcf4 // SV40-PuroR (TCF7L2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    31 first amino acids of hTcf4 have been deleted.

    Promoter

    Available since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. EdTC

    Plasmid

    #24310

    Depositor

    Roel Nusse

    Article

    Fuerer et al PLoS One. 2010 . 5(2):e9370.

    Insert

    EF1alpha-dnhTcf4 // SV40-mCherry (TCF7L2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    31 first amino acids of hTcf4 have been deleted.

    Promoter

    Available since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMpGWB103-Citrine

    Plasmid

    #79137

    Purpose

    Binary vectors for expression of Citrine fluorescent protein in M. polymorpha

    Depositor

    Yutaka Kodama

    Article

    Tsuboyama et al Plant Cell Physiol. 2014 Jan;55(1):229-3

    Insert

    Citrine
    

    Use

    Tags

    Citrine

    Expression

    Plant

    Mutation

    Promoter

    MpEF1a promoter

    Available since

    Aug. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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22. pLARRY-EGFP

    Plasmid

    #140025

    Purpose

    Lentiviral backbone for cloning expressed barcodes in the 3'UTR of the EGFP mRNA

    Depositor

    Fernando Camargo

    Article

    Weinreb et al Science. 2020 Jan 23. pii: science.aaw33

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1alpha

    Available since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLenti-EGFP-hChibby1

    Plasmid

    #89770

    Purpose

    Labels mother centrioles/basal bodies

    Depositor

    Ken-Ichi Takemaru

    Insert

    Chibby1 (CBY1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    EF1alpha

    Available since

    Sept. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. aCE120

    Plasmid

    #204491

    Purpose

    Figure 1. reporter circuit

    Depositor

    Ron Weiss

    Article

    Wauford et al Cell Syst. 2023 Sep 20;14(9):806-818.e5.

    Inserts

    EBFP2
    mKate2
    EYFP
    

    Use

    Synthetic Biology

    Tags

    none

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    hEF1a

    Available since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. aCE121

    Plasmid

    #204492

    Purpose

    Cadherin circuit

    Depositor

    Ron Weiss

    Article

    Wauford et al Cell Syst. 2023 Sep 20;14(9):806-818.e5.

    Inserts

    EBFP2
    Cdh6-mKate2
    Cdh1-EYFP
    

    Use

    Synthetic Biology

    Tags

    2A

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    hEF1a

    Available since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEBB-BT

    Plasmid

    #36295

    Depositor

    Ezra Burstein

    Article

    Maine et al Nat Protoc. 2010 Aug;5(8):1447-59. Epub

    Type

    Empty backbone

    Use

    Tags

    BT-tag (peptide sequence from P.freudenreichii tr…

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    June 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSL1045

    Plasmid

    #129525

    Purpose

    pDEF-SpCas9, Empty Vector for CRISPRi, PbEF1a promoter, 1st Generation

    Depositor

    Scott Lindner

    Article

    Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

    Insert

    dSpCas9 (Other)
    

    Use

    Plasmodium, rodent-infectious species

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-EFIa-DIO-LMO3 (sbGLuc-VChR1-EYFP)

    Plasmid

    #114105

    Purpose

    Cre-dependent fusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Insert

    sbGLuc-VChR1-EYFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJTT002

    Plasmid

    #204493

    Purpose

    Reporter circuit

    Depositor

    Ron Weiss

    Article

    Wauford et al Cell Syst. 2023 Sep 20;14(9):806-818.e5.

    Inserts

    TagBFP
    EYFP
    mKate2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    hEF1a

    Available since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHR-Traditional CAR (anti-CD19)

    Plasmid

    #192115

    Purpose

    Lentiviral delivery of Traditional CAR(anti-CD19)

    Depositor

    Wilson Wong

    Article

    Li et al Cancer Cell. 2022 Aug 26. pii: S1535-610

    Insert

    pHR-pEF1a-Traditional CAR (anti-CD19scFV_CD28_41BB_CD3z)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge