We narrowed to 7,293 results for: 11

1. pCas9_sgRNA_0

   Plasmid

   #70763

   Purpose

   expresses Ustilago maydis codon-optimized Cas9, contains U. maydis U6 promoter, is self-replicating

   Depositor

   Regine Kahmann

   Article

   Schuster et al Fungal Genet Biol. 2015 Sep 11. pii: S10

   Inserts

   U6 promoter
   cas9
   tnos terminator
   

   Use

   Self-replicating in ustilago maydis, conferring c…

   Tags

   N-terminal NLS, C-terminal HA-tag +NLS

   Mutation

   Streptococcus pyogenes gene codon-optimized for U…

   Promoter

   U6 from Ustilago maydis and synthetic otef promot…

   Available Since

   Nov. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pWPI_SPbFGF

   Plasmid

   #25812

   Depositor

   Patrick Salmon, Jozsef Kiss

   Article

   Dayer et al Brain. 2007 Nov . 130(Pt 11):2962-76.

   Insert

   pWPI_SPbFGF (FGF2 Human)
   

   Use

   Lentiviral

   Mutation

   Ig signal peptide in N-terminal of human FGF2

   Available Since

   Jan. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. STARR-seq luciferase INF enhancer vector_ORI_MX1

   Plasmid

   #99323

   Purpose

   Luciferase validation vector with MX1 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity

   Depositor

   Alexander Stark

   Article

   Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

   Insert

   enhancer; chr21: 42791443 -42793515
   

   Use

   Luciferase; Starr-seq luciferase validation vector

   Expression

   Mammalian

   Available Since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. EGFR D837A

   Plasmid

   #11014

   Purpose

   Retroviral construct for expressing human EGFR with D837A mutation in human cells

   Depositor

   Matthew Meyerson

   Article

   Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

   Insert

   EGFR D837A (EGFR Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   D837A

   Available Since

   Dec. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

5. pGL3-KRAS

   Plasmid

   #13919

   Depositor

   Frank Slack

   Article

   Johnson et al Cell. 2005 Mar 11. 120(5):635-47.

   Insert

   KRAS 3'UTR (KRAS Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   Feb. 8, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-dCas9-D3A

   Plasmid

   #78256

   Purpose

   Expresses dead Cas9 (dCas9) fused to DNMT3A catalytic domain (CD) under the control of the CMV promoter

   Depositor

   Grant Challen

   Article

   McDonald et al Biol Open. 2016 May 11. pii: bio.019067.

   Insert

   DNMT3A CD aa 598-912 (DNMT3A Human)
   

   Use

   CRISPR

   Tags

   Flag epitope tag

   Expression

   Mammalian

   Promoter

   pCMV

   Available Since

   June 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCS2-MT mouse Axin (Axin MTFu1)

   Plasmid

   #21287

   Depositor

   Frank Costantini

   Article

   Zeng et al Cell. 1997 Jul 11. 90(1):181-92.

   Insert

   Axin (Axin1 Mouse)
   

   Tags

   Myc

   Expression

   Mammalian

   Available Since

   Dec. 15, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCSIIpuro-BTC-ScNeo

   Plasmid

   #209898

   Purpose

   To monitor the status of Betacellulin, the plasmid encodes a recombinant BTC fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

   Depositor

   Michiyuki Matsuda

   Article

   Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

   Insert

   BTC-ScNeo (BTC Human)
   

   Use

   Lentiviral

   Tags

   mNeonGreen and mScarlet

   Available Since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCSIIpuro-Necl5-ScNeo

   Plasmid

   #209901

   Purpose

   To monitor the status of Necl-5, the plasmid encodes a recombinant Necl-5 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

   Depositor

   Michiyuki Matsuda

   Article

   Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

   Insert

   Necl5-ScNeo (Pvr Mouse)
   

   Use

   Lentiviral

   Tags

   mNeonGreen and mScarlet

   Available Since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. p3xFLAG-CMV-Tlr4

    Plasmid

    #27148

    Depositor

    Bruce Beutler

    Article

    Poltorak et al Science. 1998 Dec 11. 282(5396):2085-8.

    Insert

    toll-like receptor 4 (Tlr4 Mouse)
    

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Native signal peptide sequence (original amino ac…

    Available Since

    Jan. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pCD5-H5_Vietnam_1203/04_sfGFP_ST

    Plasmid

    #182546

    Purpose

    Expresses a sfGFP fused H5 protein of the prototype A/Vietnam/1203/04 virus

    Depositor

    Robert de Vries

    Article

    Broszeit et al Cell Rep. 2019 Jun 11;27(11):3284-3294.e

    Insert

    H5_Vietnam_1203_04
    

    Tags

    GCN4-TEV-sfGFP-TwinStrep

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMSCV-Flag-cMyc T58A

    Plasmid

    #20075

    Depositor

    Juan Belmonte

    Article

    Aasen et al Nat Biotechnol. 2008 Nov . 26(11):1276-8

    Insert

    cMyc (MYC Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    T58A

    Available Since

    Jan. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

13. pSF11-wNIR-GECO2-T2A-HO1

    Plasmid

    #159606

    Purpose

    Codon-optimized near-infrared fluorescent calcium indicator NIR-GECO2 with codon-optimized heme-oxygenase in C.elegans expression plasmid

    Depositor

    Robert Campbell

    Article

    Qian et al PLoS Biol. 2020 Nov 24;18(11):e3000965.

    Insert

    wNIR-GECO2-T2A-HO1
    

    Expression

    Worm

    Promoter

    tag-168

    Available Since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIRES-hrGFP II-mTET1_CD

    Plasmid

    #83571

    Purpose

    Expresses a mutant catalytic domain of TET1 in mammalian cells

    Depositor

    Jean-Pierre Issa

    Article

    Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-

    Insert

    Tet Methylcytosine Dioxygenase 1 (TET1 Human)
    

    Tags

    3X FLAG tag and hr GFP II

    Expression

    Mammalian

    Mutation

    TET1 amino acids 1-1417 deleted, catalytic domain…

    Promoter

    CMV

    Available Since

    Sept. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDH-EMTB-TurboID-V5-puro

    Plasmid

    #190741

    Purpose

    A lentiviral plasmid encoding EMTB fusion to V5-tagged TurboID on microtubules (EMTB as the microtubule-targeting tag)

    Depositor

    Jeremy Baskin

    Article

    Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021

    Insert

    EMTB-TurboID-V5 (RPS14 Human, EMTB is human ensconsin; TurboID is engineered BirA from E.coli)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Microtubule binding-domain of ensconsin (amino ac…

    Promoter

    CMV

    Available Since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. MT-AvrXa10-FT

    Plasmid

    #234373

    Purpose

    T-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-GFP-AvrXa10-GB1) with optimized activation domain (AvrXa10), targeting Arabidopsis FT locus

    Depositor

    Michael Smanski

    Article

    Zinselmeier et al Plant Biotechnol J. 2024 Nov;22(11):3202

    Inserts

    NbGP41-GFP-AvrXa10-GB1
    dCas9-24XGP41
    gRNAs targeting FT locus
    RFP
    

    Use

    Synthetic Biology

    Tags

    GB1, GFP, and GP41

    Expression

    Plant

    Promoter

    Arabidopsis U6, Arabidopsis Ubi10, and Arabidopsi…

    Available Since

    April 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMX-SHP1C/S-ires-mCD8

    Plasmid

    #183719

    Purpose

    Expresses the myc-tagged SHP-1 C453S mutant (SHP-1C/S) (substrate-trapping mutant) and mouse CD8.

    Depositor

    Takeshi Tsubata

    Article

    Alborzian Deh Sheikh et al J Immunol. 2021 Jun 1;206(11):2544-2551.

    Insert

    SHP-1
    

    Use

    Retroviral

    Tags

    myc

    Mutation

    C453S

    Available Since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hSyn-GRAB_eCBmut

    Plasmid

    #164605

    Purpose

    Express the endocannabinoid non-responsive sensor GRAB_eCBmut in neurons

    Depositor

    Yulong Li

    Article

    Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

    Insert

    Endocannabinoid non-responsive sensor GRAB_eCBmut
    

    Use

    AAV

    Mutation

    S383T, F177A

    Promoter

    hSyn

    Available Since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. FH1-PNGT#1-mCherry_PGK-H2B-CFP

    Plasmid

    #164103

    Purpose

    Fluorescent reporter for genetic tracing of proneural Glioblastoma cell-state.

    Depositor

    Gaetano Gargiulo

    Article

    Schmitt et al Cancer Discov. 2021 Mar;11(3):754-777. d

    Insert

    PNGT#1-mCherry
    

    Use

    Lentiviral and Synthetic Biology

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. CMV-FLAG-TAOK2

    Plasmid

    #197862

    Purpose

    Expression of FLAG-tagged TAOK2 / PSK1 alpha in mammalian cells

    Depositor

    Jonathan Morris

    Article

    Koo et al Mol Cancer Ther. 2017 Nov;16(11):2410-24

    Insert

    TAOK2 (TAOK2 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Wild Type

    Promoter

    SV40

    Available Since

    March 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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