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pJH1498

Plasmid

#178600

Purpose

Prgef-1 INS-18::GFP unc-54 3' UTR C.elegans pan-neural expression of INS-18 fused to GFP

Depositor

Mei Zhen

Article

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

INS-18 (ins-18 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Prgef-1

Available since

Jan. 5, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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KG#244

Plasmid

#110927

Purpose

Vector for expressing proteins in a subset of 9 DA/ DB cholingergic motor neurons in the C. elegans ventral nerve cord

Depositor

Kenneth Miller

Article

Edwards et al J Cell Biol. 2009 Sep 21;186(6):881-95.

Inserts

unc-129 promoter
unc-54 3' control region

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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KG#80

Plasmid

#110875

Purpose

Expresses the C. elegans acy-1 cDNA in body wall muscle

Depositor

Kenneth Miller

Article

Reynolds et al Genetics. 2005 Feb;169(2):651-70. doi: 1

Inserts

myo-3 promoter
acy-1 cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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pBN279

Plasmid

#86725

Purpose

Tissue-specific FLP expression plasmid for C. elegans

Depositor

Peter Askjaer

Article

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

Phlh-8::FLP D5

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pMDJ39 - Peft-3 Cre (PATCs)

Plasmid

#191381

Purpose

High efficiency CRE recombinase expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

Peft-3 Cre tbb-2 UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Peft-3

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pB-GLUT4-7myc-GFP

Plasmid

#52872

Purpose

detection and measurement of GLUT4 translocation

Depositor

Jonathan Bogan

Article

Bogan et al Mol Cell Biol. 2001 Jul;21(14):4785-806.

Insert

GLUT4 (Slc2a4 with human c-terminus, Rat)

Use

Retroviral

Tags

7x-myc (internal) and GFP

Expression

Mammalian

Mutation

Promoter

Available since

April 22, 2014

Availability

Academic Institutions and Nonprofits only

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pJG100

Plasmid

#138966

Purpose

Peft-3::wrmScarlet1-10::unc-54 3’UTR. Expresses wrmScarlet1-10 in somatic tissues of C. elegans

Depositor

Cynthia Kenyon

Article

Goudeau et al Genetics. 2021 Apr 15;217(4). pii: 61264

Insert

wrmScarlet1-10

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

July 7, 2020

Availability

Academic Institutions and Nonprofits only

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pSEZ-1

Plasmid

#51868

Purpose

Expression of cpYFP with a mitochondrial-targeting sequence (mt-cpYFP) in C. elegans

Depositor

Meng-Qiu Dong

Article

Shen et al Nature. 2014 Feb 12. doi: 10.1038/nature

Insert

psdhb-1::mtLS-cpYFP

Use

Tags

Expression

Worm

Mutation

Promoter

sdhb-1

Available since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

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FRT LAMP3 mCRISPRed_mTagBFP2 pcDNA5

Plasmid

#159455

Purpose

Encodes a lysosome tagret fusion protein of the pH resistant proteins mCRISPRed and mTagBFP2

Depositor

Oliver Griesbeck

Article

Erdogan et al Cell Chem Biol. 2020 May 21;27(5):610-62

Insert

mCRISPRed

Use

Tags

mTagBFP2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ2092 - [1-2] ENTR - Fluor - mNeon(PATC)

Plasmid

#159853

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - mNeon(PATC)

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Nr5a1

Plasmid

#41081

Depositor

Rudolf Jaenisch

Article

Buganim et al Cell Stem Cell. 2012 Sep 7;11(3):373-86.

Insert

Nr5a1 (Nr5a1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TetO + minimal CMV

Available since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

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Prab-3::NLS::GCaMP6s

Plasmid

#68119

Purpose

Nuclear Localized Fluorescent Calcium Indicator for pan-neuronal expression in C. Elegans

Depositor

Andrew Leifer

Article

Nguyen et al Proc Natl Acad Sci U S A. 2015 Dec 28. p

Insert

rab-3::NLS::GCaMP6s

Use

Tags

Expression

Worm

Mutation

Promoter

rab-3

Available since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pDYH5

Plasmid

#52032

Purpose

Expression of GFP-tagged DAF-16 in C. elegans

Depositor

Meng-Qiu Dong

Article

Tao et al Elife. 2013;2:e00518. doi: 10.7554/eLife

Insert

DAF-16 (daf-16 Nematode)

Use

Tags

GFP and HIS

Expression

Worm

Mutation

Promoter

Available since

May 6, 2014

Availability

Academic Institutions and Nonprofits only
pLentiCRISPRv2-TRAF6-targeting sgRNA 1

Plasmid

#131345

Purpose

gRNA targeting mouse TRAF6

Depositor

Jonathan Kagan

Article

Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

Insert

gRNA targeting mouse TRAF6 (Traf6 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pMLS716

Plasmid

#188888

Purpose

Targeting vector for insertion into 5' miniMos Arm; carries floxed Cbr-unc-119 marker and Phsp16.41::Cre

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Insert

floxed Cbr-unc-119 marker and Phsp16.41::Cre

Use

Tags

Expression

Worm

Mutation

C. elegans codom optimization and intron addition

Promoter

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pBN154

Plasmid

#86718

Purpose

FLP/Frt dual color reporter for C. elegans

Depositor

Peter Askjaer

Article

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

Phsp16.41::FRT::mCh::his-58::FRT::gfp::his-58

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pSN444

Plasmid

#108331

Purpose

Expresses Sat2 for Nourseothricin resistance selection of C. elegans

Depositor

Shinsuke Niwa

Article

Obinata et al PLoS One. 2018 May 9;13(5):e0197128. doi

Insert

Streptothricin acetyl transferase 2

Use

Tags

Expression

Worm

Mutation

Promoter

rps-27 promoter

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-TRAF6-targeting sgRNA 2

Plasmid

#131346

Purpose

gRNA targeting mouse TRAF6

Depositor

Jonathan Kagan

Article

Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

Insert

gRNA targeting mouse TRAF6 (Traf6 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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KG#121

Plasmid

#110879

Purpose

Expresses the C. elegans unc-31 cDNA pan-neuronally

Depositor

Kenneth Miller

Article

Charlie et al Genetics. 2006 Feb;172(2):943-61. doi: 1

Inserts

rab-3 promoter
unc-31 cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region

Use

Tags

Expression

Bacterial

Mutation

Missing first 408bp

Promoter

Available since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-3xFLAG-MyD88-V5

Plasmid

#131351

Purpose

Expresses MyD88 tagged with a 3xFLAG tag at its N-terminus and a V5 tag at its C-terminus

Depositor

Jonathan Kagan

Article

Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

Insert

MyD88 (Myd88 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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