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HA Display Part 1-4 DO

Plasmid

#206514

Purpose

CcdB Dropout vector for parts 1-4 of Hemagglutinin surface expression in mammalian cells

Depositor

IIya Finkelstein

Type

Empty backbone

Tags

3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…

Expression

Mammalian

Promoter

CMV promoter

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pET28b-TREER

Plasmid

#208321

Purpose

Bacterial expression of the His-tagged peptide TREER.

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TREER

Tags

DBCO-Tag, His-Tag

Expression

Bacterial

Available Since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pET28b-PuRRRE

Plasmid

#208323

Purpose

Bacterial expression of the His-tagged peptide PuRRRE.

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

PuRRRE

Tags

DBCO-Tag, His-Tag

Expression

Bacterial

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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XLone-mARG2

Plasmid

#173792

Purpose

Mammalian acoustic reporter gene 1 cassette 2 on XLone plasmid

Depositor

Xiaojun Lian

Article

Howells et al Bioeng Transl Med. 2023 Aug 2;9(2):e1058

Insert

GvpN, F G L S K J U

Tags

GFP

Expression

Mammalian

Promoter

TRE3G promoter

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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IRE-miR21mutant-SunTag-TREAT

Plasmid

#196933

Purpose

SunTag cassette was added in the coding region in the miR21mutant -IRE-TREAT reporter.

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Expression

Bacterial and Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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IRE-let7-SunTag-TREAT

Plasmid

#196926

Purpose

SunTag cassette was added in the coding region in the let7-IRE-TREAT reporter

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Expression

Bacterial and Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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IRE-let7R-SunTag-TREAT

Plasmid

#196927

Purpose

SunTag cassette was added in the coding region in the let7R-IRE-TREAT reporter.

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Expression

Bacterial and Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pMpGE010-Target1 (Mpphot)

Plasmid

#186728

Purpose

Binary vector for CRISPR/Cas9 (target 1: Mpphot [positive control]) in plants (for Agrobacterium-mediated genetic transformation).

Depositor

Yutaka Kodama

Article

Konno et al Biochem Biophys Res Commun. 2018 Sep 3;5

Insert

Mphot

Expression

Bacterial

Available Since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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KTK_346

Plasmid

#180549

Purpose

Entry vector containing gfasPurple

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

gfasPurple

Expression

Bacterial

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-FRT-2xtTA

Plasmid

#191205

Purpose

Flpo dependent tTA expression under the control of TRE promotor

Depositor

Janelia Viral Tools

Article

Glaser et al Nat Methods. 2022 May;19(5):613-619. doi

Insert

2xtTA

Use

AAV

Expression

Mammalian

Promoter

TRE

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pMpGE010-Target2 (Mpphot)

Plasmid

#186729

Purpose

Binary vector for CRISPR/Cas9 (target 2: Mpphot [negative control]) in plants (for Agrobacterium-mediated genetic transformation).

Depositor

Yutaka Kodama

Article

Konno et al Biochem Biophys Res Commun. 2018 Sep 3;5

Insert

Mphot

Expression

Bacterial

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pMpGE_En03-sgRNA_Target2 (Mpphot)

Plasmid

#186727

Purpose

Gateway entry vector for sgRNA (target 2: Mpphot [negative control]). Transient expression of sgRNA (target 2: Mpphot) in plant cells.

Depositor

Yutaka Kodama

Article

Konno et al Biochem Biophys Res Commun. 2018 Sep 3;5

Insert

sgRNA_Mphot

Expression

Bacterial

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pMpGE_En03-sgRNA_Target1 (Mpphot)

Plasmid

#186726

Purpose

Gateway entry vector for sgRNA (target 1: Mpphot [positive control]). Transient expression of sgRNA (target 1: Mpphot) in plant cells.

Depositor

Yutaka Kodama

Article

Konno et al Biochem Biophys Res Commun. 2018 Sep 3;5

Insert

sgRNA_Mphot

Expression

Bacterial

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pET51b_SNAP-ecDHFRmt

Plasmid

#187109

Purpose

Expression of SNAP-ecDHFRmt fusion in bacteria

Depositor

Kai Johnsson

Article

Wang et al Angew Chem Int Ed Engl. 2020 Dec 1;59(49

Insert

SNAP-ecDHFRmt

Tags

Hisx10 and Strep

Expression

Bacterial

Mutation

ecDHFR-R44L-H45Q

Promoter

T7

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_345

Plasmid

#180550

Purpose

Entry vector containing eforRed

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

eforRed

Expression

Bacterial

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_036

Plasmid

#180558

Purpose

Entry vector containing L3S1P13 terminator

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

L3S1P13 terminator

Expression

Bacterial

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_040

Plasmid

#180562

Purpose

Entry vector containing L3S2P24 terminator

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

L3S2P24 terminator

Expression

Bacterial

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_029

Plasmid

#180542

Purpose

Entry vector containing RBS BBa_B0035

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

RBS BBa_B0035

Expression

Bacterial

Available Since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_034

Plasmid

#180526

Purpose

Entry vector containing pLux promoter

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

pLux promoter

Expression

Bacterial

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_035

Plasmid

#180557

Purpose

Entry vector containing L3S1P00 terminator

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

L3S1P00 terminator

Expression

Bacterial

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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