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iDianiSnFR

Plasmid

#177741

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Publication

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

iDianiSnFR

Tags

HA, His, and Myc

Expression

Bacterial

Promoter

T7

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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iCytSnFR

Plasmid

#177738

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Publication

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

iCytSnFR

Tags

HA, His, and Myc

Expression

Bacterial

Promoter

T7

Available since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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iCyt_BrEt_SnFR

Plasmid

#177740

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Publication

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

iCyt_BrEt_SnFR

Tags

HA, His, and Myc

Expression

Bacterial

Promoter

T7

Available since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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iCyt_F_SnFR

Plasmid

#177739

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Publication

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

iCyt_F_SnFR

Tags

HA, His, and Myc

Expression

Bacterial

Promoter

T7

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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MPC021: CamKII CMV_sNovArch_citrine (soma targeting)

Plasmid

#153194

Purpose

encoded soma targeted NovArch

Depositor

Adam Cohen

Publication

Chien et al Sci Adv. 2021 May 5;7(19). pii: 7/19/eab

Insert

sNovArch

Tags

citrine

Expression

Mammalian

Available since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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ROPY-4A

Plasmid

#128005

Purpose

Unphosphorylatable mutant of Stathmin/OP18 FRET-sensor

Depositor

Dorus Gadella

Publication

Van Geel et al Sci Rep. 2020 Apr 7;10(1):6034. doi: 10.

Insert

ROPY-4A

Expression

Mammalian

Mutation

S16A, S25A, S38A, S63A in OP18

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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ROPY-4E

Plasmid

#128006

Purpose

Phosphomimic mutant of Stathmin/OP18 FRET-sensor

Depositor

Dorus Gadella

Publication

Van Geel et al Sci Rep. 2020 Apr 7;10(1):6034. doi: 10.

Insert

ROPY-4E

Expression

Mammalian

Mutation

S16E, S25E, S38E, S63E in OP18

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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BSP609

Plasmid

#122254

Purpose

expression vector for mTFP under EFT3 promoter

Depositor

Alexander Mendenhall

Publication

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

mTFP

Expression

Worm

Promoter

EFT3

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDL86

Plasmid

#43909

Depositor

Stephen Elledge

Publication

Lee et al Mol Cell. 2008 Oct 10;32(1):70-80. doi:

Insert

DIF1 (DIF1 Budding Yeast)

Expression

Yeast

Available since

June 10, 2014

Availability

Academic Institutions and Nonprofits only
co LuxDEC(KZK)

Plasmid

#253533

Purpose

Express luciferin biosynthesis of human codon-optimized LuxDEC in mammalian cells

Depositor

Takeharu Nagai

Publication

Kusuma et al Proc Natl Acad Sci U S A. 2024 Oct 8;121

Insert

co LuxD-P2A-co LuxE-T2A-co LuxC

Expression

Mammalian

Promoter

CMV

Available since

April 10, 2026

Availability

Academic Institutions and Nonprofits only

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co LuxB-Frp (G4S)

Plasmid

#253532

Purpose

Express human codon-optimized of LuxB-G4S-Frp in mammalian cells

Depositor

Takeharu Nagai

Publication

Kusuma et al Proc Natl Acad Sci U S A. 2024 Oct 8;121

Insert

co LuxB-Frp(G4S)

Expression

Mammalian

Promoter

CMV

Available since

April 10, 2026

Availability

Academic Institutions and Nonprofits only

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sREACh-C1

Plasmid

#21946

Purpose

For expressing or cloning fusion proteins with super-REACh (sREACH, aka Nữ), improved non-radiative YFP

Depositor

Ryohei Yasuda

Publication

Murakoshi et al Brain Cell Biol. 2008 Aug . 36(1-4):31-4

Insert

sREACh

Expression

Mammalian

Available since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pFSW-BsmBI-shRNA-hSyn-GFP

Plasmid

#220436

Purpose

Lentiviral shRNA cloning backbone with GFP

Depositor

Paul Blainey

Publication

Le et al Cell Rep. 2025 Jun 24;44(7):115889. doi:

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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HaloXRCC4 sgRNA

Plasmid

#207606

Purpose

sgRNA for the insert of the HaloTag at the endogenous loci of XRCC4.

Depositor

Jens Schmidt

Publication

Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

Insert

TACTGGGTTCAGAAACAAGG

Expression

Mammalian

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pB-DEST (JDW 1205)

Plasmid

#229821

Purpose

A PiggyBac destination vector compatible with gateway cloning system.

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Expression

Mammalian

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pXMLC2-DEST (JDW 7)

Plasmid

#229833

Purpose

A gateway compatible destination vector containing the Xenopous myosin light chain 2 promoter for embryonic and adult cardiomyocyte expression.

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Use

Gateway Cloning

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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fabp10a:FLAG-SpiCee-mRFP1; cryaa:mCherry

Plasmid

#230062

Purpose

Expression of genetically encoded calcium chelator in zebrafish hepatocytes.

Depositor

Sumeet Pal Singh

Publication

Pozo-Morales et al Hepatology. 2023 Mar 1;77(3):789-801. do

Insert

SpiCee

Use

Zebrafish expression

Tags

FLAG and mRFP1

Promoter

fabp10a

Available since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pN1-BPEK

Plasmid

#129313

Purpose

ATP measurement

Depositor

Yasushi Okada

Publication

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN-BPEK

Expression

Mammalian

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pAG369-lyn11-redFAST

Plasmid

#160356

Purpose

Expresses Lyn11-redFAST in mammalian cells

Depositor

Arnaud Gautier

Publication

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

lyn11-redFAST

Expression

Mammalian

Available since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pAG361-lyn11-greenFAST

Plasmid

#160352

Purpose

Expresses Lyn11-greenFAST in mammalian cells

Depositor

Arnaud Gautier

Publication

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

lyn11-greenFAST

Expression

Mammalian

Available since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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