We narrowed to 6,471 results for: poly

1. pcDNA3.1-Zeo_BACE1

   Plasmid

   #227105

   Purpose

   BACE1 enzyme overexpression for Nrg1 activation in luciferase and barcode assays

   Depositor

   Michael Wehr

   Article

   Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

   Insert

   BACE1 (BACE1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMXS-IRES-Blast POLA1

   Plasmid

   #160807

   Purpose

   Express POLA1

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   POLA1 (POLA1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Codon Optimized

   Promoter

   Available since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV_GR-RE_MLPmin_BC1092-luc2

   Plasmid

   #227112

   Purpose

   Glucocorticoid receptor response element for luciferase and barcode assays; barcode BC1092

   Depositor

   Michael Wehr

   Article

   Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

   Insert

   12x clustered GR response element linked to MLP minimal promoter driving barcode 1092 and a luciferase reporter gene
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-hCdt1-HA

   Plasmid

   #117500

   Purpose

   Expresses human Cdt1 fused to HA tag

   Depositor

   Domenico Maiorano

   Article

   Tsanov et al Nucleic Acids Res. 2014 Apr;42(6):3692-7

   Insert

   Cdc10-dependent transcript-1 (CDT1 1700 bp, Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   wild-type

   Promoter

   CMV

   Available since

   Jan. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET32a-HD16Q

   Plasmid

   #11487

   Depositor

   Pamela Bjorkman

   Article

   Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

   Insert

   huntingtin, exon 1, 16 glutamines (HTT Human)
   

   Use

   Tags

   His, His (out of frame), and Trx

   Expression

   Bacterial

   Mutation

   huntingtin exon 1 with 16 glutamines, vector m…

   Promoter

   Available since

   April 28, 2006

   Availability

   Academic Institutions and Nonprofits only

6. Frt-V5-hspB5

   Plasmid

   #63106

   Purpose

   Expression of HSPB5 (small HSP) tagged with V5 in mammalian cells

   Depositor

   Harm Kampinga

   Article

   Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

   Insert

   hspb5 (CRYAB Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLJM1-Flag-Rheb-4Rs

   Plasmid

   #165022

   Purpose

   expression of Flag-Rheb-4Rs mutant protein in mammalian cells (the lysine residues on the position 109, 135, 151 and 178 on Rheb protein are mutated to arginines)

   Depositor

   Ken Inoki

   Article

   Yao et al Mol Cell. 2020 Nov 5;80(3):437-451.e6. d

   Insert

   Rheb-4Rs (RHEB Human)
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   change lysine residues on position 109, 135, 151,…

   Promoter

   Available since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV_AR-RE_MLPmin_BC1011-luc2

   Plasmid

   #227106

   Purpose

   Androgen receptor response element for luciferase and barcode assays; barcode BC1011

   Depositor

   Michael Wehr

   Article

   Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

   Insert

   8x clustered AR response element linked to MLP minimal promoter driving barcode 1011 and a luciferase reporter gene
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV_AR-RE_MLPmin_BC1014-luc2

   Plasmid

   #227108

   Purpose

   Androgen receptor response element for luciferase and barcode assays; barcode BC1014

   Depositor

   Michael Wehr

   Article

   Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

   Insert

   8x clustered AR response element linked to MLP minimal promoter driving barcode 1014 and a luciferase reporter gene
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLIB-HIS-TEV-CKS1

    Plasmid

    #177013

    Purpose

    Generate baculovirus for insect cell expression of CKS1 with N-terminal Polyhistidine-TEV

    Depositor

    Andrea Musacchio

    Article

    Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

    Insert

    CKS1 (CKS1B Human, Synthetic)
    

    Use

    Tags

    Polyhistidine-TEV

    Expression

    Insect

    Mutation

    codon-optimised for insect cell expression

    Promoter

    Available since

    Nov. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pK27Sumo_His-SUMO-nsp7 (SARS-CoV-2)

    Plasmid

    #169186

    Purpose

    To express His-SUMO-nsp7 (SARS-CoV-2) in E. coli

    Depositor

    John Diffley

    Article

    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

    Insert

    14His-SUMO-nsp7 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    14His-SUMO (Ulp1-cleavable)

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T5

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. hu-TLR4 399 SNP flag pLenti4/To/v5/DEST

    Plasmid

    #42651

    Purpose

    destination vector for lentivirus-mediated expression of human TLR4 399 SNP in mammalian cells

    Depositor

    Scott Friedman

    Article

    Guo et al Hepatology. 2009 Mar;49(3):960-8. doi: 1

    Insert

    TLR4 399 SNP (TLR4 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    T399I

    Promoter

    CMV/TO

    Available since

    March 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-UbC

    Plasmid

    #62806

    Purpose

    To express genes at high levels in neuronal cells. This UbC promoter is more active in neurons than the promoter in CMV-based vectors.

    Depositor

    Pantelis Tsoulfas, Jae Lee

    Article

    Soderblom et al Eneuro. 2015 Mar-Apr;2(2). doi: 10.1523/

    Type

    Empty backbone

    Use

    AAV; Eucaryotic

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available since

    March 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV_ER-RE_MLPmin_BC1063-luc2

    Plasmid

    #227109

    Purpose

    Estrogen receptor response element for luciferase and barcode assays; barcode BC1063

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    12x clustered ER response element linked to MLP minimal promoter driving barcode 1063 and a luciferase reporter gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV_ER-RE_MLPmin_BC1065-luc2

    Plasmid

    #227111

    Purpose

    Estrogen receptor response element for luciferase and barcode assays; barcode BC1065

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    12x clustered ER response element linked to MLP minimal promoter driving barcode 1065 and a luciferase reporter gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV_GR-RE_MLPmin_BC1094-luc2

    Plasmid

    #227114

    Purpose

    Glucocorticoid receptor response element for luciferase and barcode assays; barcode BC1094

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    12x clustered GR response element linked to MLP minimal promoter driving barcode 1094 and a luciferase reporter gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCR8/GW_TOPO_Cdk12_Nterm_FlagHa

    Plasmid

    #127177

    Purpose

    N-terminal Flag- HA- tandem epitope tagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into Gateway entry vector

    Depositor

    Phil Sharp

    Article

    Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

    Insert

    N-terminally Flag- HA- Epitope Tagged Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
    

    Use

    Entry vector with transgene for gateway cloning

    Tags

    Flag- HA- Tandem Epitopes

    Expression

    Mutation

    Promoter

    None

    Available since

    July 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBK086

    Plasmid

    #183145

    Purpose

    pET-6xHis-MBP-TEV-MapTIR-APAZ/MapAgo - Heterolgous MapSPARTA expression

    Depositor

    Daan Swarts

    Article

    Koopal et al Cell. 2022 Apr 28;185(9):1471-1486.e19.

    Insert

    MapSPARTA operon (6xHis-MBP-MapTIR-APAZ and MapAgo)
    

    Use

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCDH-EF1-FHC-POLQ-K121M

    Plasmid

    #64877

    Purpose

    lentiviral expression of human POLQ K121M mutant (a mutation in the conserved ATP-binding site of the Walker A motif in the helicase-like domain)

    Depositor

    Richard Wood

    Article

    Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

    Insert

    POLQ (POLQ Human)
    

    Use

    Lentiviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    K121M, a mutation in the conserved ATP-binding si…

    Promoter

    EF1alpha

    Available since

    Oct. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBabe-Puro-IKBalpha-wt

    Plasmid

    #15290

    Depositor

    William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    inhibitor of Kappa B alpha (NFKBIA Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only