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pJUMP19-L3S1P51_T

Plasmid

#127037

Purpose

Basic Part T- Terminator; L3S1P51; synthetic terminator

Depositor

Chris French

Publication

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part L3S1P51_T

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP19-J23102_P

Plasmid

#126997

Purpose

Basic Part P- Promoter (constitutive); J23102.

Depositor

Chris French

Publication

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part J23102_P

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP18-cat_TU

Plasmid

#127051

Purpose

Basic Part Whole TU; Codes for chloramphenicol resistance; including promoter and terminator. Broad host range. From Bacillus/E.coli shuttle pSEVA3b61.

Depositor

Chris French

Publication

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part Cat_TU

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP19-L3S1P51_CT

Plasmid

#127047

Purpose

Basic Part CT- C-terminus + Terminator; L3S1P51. C-termini STOP + terminator (synthetic)

Depositor

Chris French

Publication

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part L3S1P51_CT

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP18-Streptag_C

Plasmid

#127036

Purpose

Basic Part C- C-terminus; Strep-tag II

Depositor

Chris French

Publication

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part StrepII_tag_C

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP18-Histag_C

Plasmid

#127035

Purpose

Basic Part C- C-terminus; polyhistidine tag

Depositor

Chris French

Publication

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part His6_C

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP18-Streptag_N

Plasmid

#127034

Purpose

Basic Part N- N-terminus; Strep-tag II

Depositor

Chris French

Publication

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part StrepII_tag_N

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP18-cueR_O

Plasmid

#127026

Purpose

Basic Part O- ORF; cueR; transcription factor

Depositor

Chris French

Publication

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part cueR_o

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP18-tetR_O

Plasmid

#127021

Purpose

Basic Part O- ORF; TetR; transcription factor

Depositor

Chris French

Publication

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part TetR_o

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP19-Ptet_P

Plasmid

#127005

Purpose

Basic Part P- Promoter (non-constitutive); Ptet; induced by tetracycline/analogue; controlled by TetR

Depositor

Chris French

Publication

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part Ptet_P

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP19-Pbad_P

Plasmid

#127003

Purpose

Basic Part P- Promoter (non-constitutive); Pbad (I13453), arabinose induced; controlled by AraC.

Depositor

Chris French

Publication

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part Pbad

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP19-T7pro_P

Plasmid

#127002

Purpose

Basic Part P- Promoter (non-constitutive); T7 promoter; recognized by T7 polymerase

Depositor

Chris French

Publication

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part T7 promoter_P

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP19-J23107_P

Plasmid

#127000

Purpose

Basic Part P- Promoter (constitutive); J23107.

Depositor

Chris French

Publication

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part J23107_P

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP19-J23100_P

Plasmid

#126996

Purpose

Basic Part P- Promoter (constitutive); J23100

Depositor

Chris French

Publication

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part J23100_P

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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KO801: pMVP (L5-L4) Hygro-P2A

Plasmid

#121718

Purpose

pMVP L5-L4 entry plasmid, contains Hygro-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term Hygro selection marker linked by P2A to gene of interest.

Depositor

Christopher Newgard

Publication

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

Hygro-P2A (open)

Use

Gateway Cloning and Synthetic Biology

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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IY704: pMVP (L1-R5) EF1a promoter

Plasmid

#121693

Purpose

pMVP L1-R5 entry plasmid, contains EF1a promoter for 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Publication

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

EF1a promoter

Use

Gateway Cloning and Synthetic Biology

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pAP608-1

Plasmid

#99484

Purpose

H2B::linker::GFP (contain 3 introns)::3XFlag::TEV::Myc::tbb-2 3UTR

Depositor

Geraldine Seydoux

Publication

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

H2B::linker::GFP with introns::3XFlag::TEV::Myc::tbb-2 3UTR

Expression

Bacterial

Available since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pLSLZ.D

Plasmid

#51496

Purpose

pLSL with Zif268:FokI coding sequence followed by us:NPTII repair template , Rep is not included on this plasmid

Depositor

Daniel Voytas

Publication

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Inserts

Zif268:FokI
us:NPTII repair template

Use

Plant t-dna plasmid

Tags

SV40 NLS

Promoter

virion-sense LIR and 2x35S

Available since

May 2, 2014

Availability

Academic Institutions and Nonprofits only

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dSpCas9-SmBiT

Plasmid

#236759

Purpose

Split NanoLuc luciferase biosensor fused to dSpCas9

Depositor

David Segal

Publication

Heath et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

NanoLuc SmBit

Tags

dSpCas9

Expression

Mammalian

Promoter

CMV

Available since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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dSaCas9-NLuc

Plasmid

#238947

Purpose

C-terminal fusion of NanoLuc luciferase to dSaCas9, mammalian expression

Depositor

David Segal

Publication

Heath et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

NanoLuc

Tags

dSaCas9

Expression

Mammalian

Promoter

CMV

Available since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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