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We narrowed to 18,759 results for: nls

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Showing: 8881 - 8920 of 18759 results

  6. pPB9v1

    Plasmid
    #210028
    Purpose
    Contains Cas9 gRNA cloning site, inducible Cas9, rtTA-T2A-puro
    Depositor
    Michael Talkowski
    Publication
    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas9
    rtta-T2A-puro
    Use
    CRISPR
    Tags
    SV40 NLS and nucleoplasmin NLS
    Expression
    Mammalian
    Promoter
    EF-1α and TRE3G
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    210028-plasmid-map-sequence-id-413997

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  8. pLV-SFFV-KTN1-WPRE-UbC-Emerald

    Plasmid
    #225941
    Purpose
    Lentiviral vector plasmid expressing human kinectin 1 (KNT1) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis, Evan Reid
    Inserts
    KTN1 (KTN1 Human)
    Emerald
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    SFFV and UbC
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225941-plasmid-map-sequence-id-446792

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  10. pC178: pAAV.U6.SapI.CMV.Cas13bt3

    Plasmid
    #204215
    Purpose
    Plasmid AAV vector expressing Cas13bt3 and hU6-driven expression of guide RNAs. Contains SapI sites for guide cloning flanked by 5' and 3' full-length DRs.
    Depositor
    Guei-Sheung Liu
    Publication
    Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121
    Insert
    Cas13bt3 and U6.SapI sgRNA cloning site
    Use
    AAV and CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Mutation
    Codon optimisation by GenScript tool
    Promoter
    CMV/U6
    Available since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    204215-plasmid-map-sequence-id-455913

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  11. pAGK62

    Plasmid
    #228122
    Purpose
    Express node 1531 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells
    Depositor
    Seth Shipman
    Publication
    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103
    Insert
    Retron node 1531 split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
    Expression
    Mammalian
    Promoter
    CAG for RT, U6/H1 for ncRNA/gRNA
    Available since
    Dec. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    228122-plasmid-map-sequence-id-455403

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  13. pEJS2708-CMV-Nme2Smu-ABE8e-i1-L10-E932R

    Plasmid
    #228729
    Purpose
    Expresses domain inlaid Nme2Smu nCas9 (D16A)-i1-ABE8e base editor with linker-10 and E932R mutation in mammalian cells
    Depositor
    Erik Sontheimer
    Publication
    Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Domain inlaid Nme2Smu nCas9 (D16A)-i1-L10-E932R
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Nme2-Cas9, Smu PID, D16A E932R
    Promoter
    CMV
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    228729-plasmid-map-sequence-id-454007

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  16. pX330-PITCh-VIM

    Plasmid
    #227301
    Purpose
    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of VIM for knock-in.
    Depositor
    Laurence Pelletier
    Publication
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA Targeting C-terminus of VIM (VIM Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Nov. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    227301-plasmid-map-sequence-id-451733

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  18. pB-3xFlag-dCas13-mTET2CD

    Plasmid
    #228234
    Purpose
    For targeted tethering of the catalytic domain of mouse TET2 using dCas13
    Depositor
    Chuan He
    Publication
    Zou et al Nature. 2024 Oct 2. doi: 10.1038/s41586-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    dCas13
    TET2 (Tet2 Mouse)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    CD (cysteine-rich, dioxygenase domain) truncation…
    Promoter
    CAG
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    228234-plasmid-map-sequence-id-451264

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  22. pGW_RUBY_EggCas9

    Plasmid
    #225990
    Purpose
    To clone entry vector with CRISPR guide RNA via Gateway cloning; it has an Egg cell-specific expression of Cas9 and the plant is selected by the red color due to constitutive expression of RUBY
    Depositor
    Imran Siddiqi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Available since
    Oct. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    225990-plasmid-map-sequence-id-448019

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  23. pHEE-R-hyg9si

    Plasmid
    #222832
    Purpose
    CRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, EC1 promoter, hygromycin and red fluorescent seed selection)
    Depositor
    Hidenori Takeuchi
    Type
    Empty backbone
    Use
    CRISPR
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    222832-plasmid-map-sequence-id-446482

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  26. pDM026

    Plasmid
    #216808
    Purpose
    Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and pyrG selectable marker.
    Depositor
    Ken Sawin
    Publication
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Type
    Empty backbone
    Use
    CRISPR; Fungal expression
    Expression
    Bacterial
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216808-plasmid-map-sequence-id-429385

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Showing: 8881 - 8920 of 18759 results