We narrowed to 2,326 results for: PUC19

1. LADL Bridge + Promoter Only Target

   Plasmid

   #127669

   Purpose

   Encodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter as well as 2 gRNAs targeting the Zfp462 promoter expressed from their individual hU6 promoters

   Depositor

   Jennifer Phillips-Cremins

   Article

   Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

   Insert

   CRY2-HA-2A-mCherry and gRNAs 115 and 117
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a and hU6

   Available Since

   July 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDV12 [psng-1::SNG-1::CRY2olig(535)]

   Plasmid

   #197596

   Purpose

   Pan-neuronal expression of SNG-1::CRY2olig(535) in neurons of C. elegans

   Depositor

   Alexander Gottschalk

   Article

   Vettkotter et al Nat Commun. 2022 Dec 19;13(1):7827. doi:

   Insert

   Synaptogyrin-CRY2 (E490G + residues 1-535) (CRY2, sng-1 Mustard Weed, Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   E490G

   Promoter

   Available Since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIRHD-TetR-GAL80-Rho1-GAL4

   Plasmid

   #218274

   Purpose

   Introduction of low-temperature-inducible GAL system, LowTempGAL v2.2

   Depositor

   Christopher Howard

   Article

   Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

   Insert

   gal80Arm1-pAgTEF1>KanMX4>tAgTEF1-pHAC1>UBI4>>DHFRP66L-TetR>NLS>TUP1>tABF1-pTEF2>Rho1C17R>>GAL4>tGAL4-pTEF1(TetO)>GAL80Arm2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   DHFR (P66L), Rho1 (C17R)

   Promoter

   Available Since

   May 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIRHDGAL43C

   Plasmid

   #218279

   Purpose

   Introduction of a LowTempGAL Turbo module (HD-GAL43C) to accelerate induction of GAL promoter induction

   Depositor

   Claudia Vickers

   Article

   Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

   Insert

   fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pNRG2>GAL4>tNRG2-PENO2> UBI4>RHGSGTMV>DHFRP66L>G*6>GAL3C(S509P)-tENO2-fcy1(454-778)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   GAL3 (S509P), DHFR (P66L)

   Promoter

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIRGAL43C

   Plasmid

   #218278

   Purpose

   Introduction of a LowTempGAL Turbo module (GAL43C) to accelerate induction of GAL promoter induction

   Depositor

   Claudia Vickers

   Article

   Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

   Insert

   fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pNRG2>GAL4>tNRG2-pENO2>GAL3C(S509P)>tENO2-fcy1(454-778)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   GAL3 (S509P)

   Promoter

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIRHDGAL3C

   Plasmid

   #218276

   Purpose

   Introduction of a LowTempGAL Turbo module (HD-GAL3C) to accelerate induction of GAL promoter induction

   Depositor

   Claudia Vickers

   Article

   Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

   Insert

   fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pENO2> UBI4>RHGSGTMV>DHFRP66L>G*6>GAL3C(S509P)>tENO2-fcy1(454-778)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   GAL3 (S509P), DHFR (P66L)

   Promoter

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIRGAL3C

   Plasmid

   #218275

   Purpose

   Introduction of a LowTempGAL Turbo module (GAL3C) to accelerate induction of GAL promoter induction

   Depositor

   Claudia Vickers

   Article

   Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

   Insert

   fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pENO2>GAL3C(S509P)>tENO2-fcy1(454-778)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   GAL3 (S509P)

   Promoter

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Donor_YTHDF2_KO_Neo

   Plasmid

   #186672

   Purpose

   Donor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. Neomycin and polyA sequence is inserted between two homology arms.

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   YTHDF2 KO Neomycin (YTHDF2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Donor_ANKRD1_KO_Hygro

   Plasmid

   #186668

   Purpose

   Donor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. eEF1a promoter, hygromycin and polyA sequence is inserted between two homology arms.

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   ANKRD1 KO Hygromycin (ANKRD1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNOC_dfnCas12a-KRAB_crRNA Td2

    Plasmid

    #176261

    Purpose

    pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged to the KRAB domain and Nlux and crRNA targeting the fluorescent gene tdtomato with spacer 2

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    Kruppel associated box domain and luciferase

    Expression

    Mutation

    D917A and E1006A mutations to inactivate the endo…

    Promoter

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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