We narrowed to 13,642 results for: NTS

1. hSyn1-eYFP

   Plasmid

   #117382

   Purpose

   An AAV genome that expresses the fluorescent protein eYFP from the hSyn1 promoter

   Depositor

   Viviana Gradinaru

   Article

   Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

   Has Service

   AAV PHP.eB, AAV Retrograde, AAV2, AAV5, AAV8, and AAV9

   Insert

   EYFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn1

   Available since

   Oct. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. CMV-LUC2CP/ARE

   Plasmid

   #62857

   Purpose

   Splicing reporter control (no intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.

   Depositor

   Gideon Dreyfuss

   Article

   Younis et al Mol Cell Biol. 2010 Apr;30(7):1718-28. d

   Insert

   Luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   destabilizing sequences (DS) added to the C termi…

   Promoter

   CMV

   Available since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. p3015b

   Plasmid

   #209317

   Purpose

   Expresses a sgRNA template with a protospacer cloning site flanked by BsaI restriction sites for easy insertion of a targeting cassette.

   Depositor

   Thomas Hooven

   Article

   Gopalakrishna et al Commun Biol. 2023 Jun 9;6(1):620. doi: 1

   Insert

   Group B Streptococcus-compatible single guide RNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Xyl/tet promoter that is constitutively active

   Available since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCEP4-myc-HLA-A2

   Plasmid

   #135504

   Purpose

   Mammalian expression of myc-tagged HLA-A*02:01

   Depositor

   Erik Procko

   Article

   McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

   Insert

   HLA-A*02:01 (HLA-A Human)
   

   Use

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. CBE-T1.46

   Plasmid

   #193284

   Purpose

   Expression of CBE-T1.46 base editor in mammalian cells, CMV promoter

   Depositor

   Nicole Gaudelli

   Article

   Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

   Insert

   CBE-T1.46
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D10A (Cas9), TadA mutations within

   Promoter

   CMV

   Available since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-hACE2-LgBiT

   Plasmid

   #215837

   Purpose

   Expresses hACE2 receptor with LgBiT tag in mammalian cells

   Depositor

   Thomas Gallagher

   Article

   Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

   Insert

   hACE2-LgBiT (ACE2 )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-PS9

   Plasmid

   #177944

   Purpose

   Transient mammalian expression of eGFP along with SARS-CoV-2 sequence 20080-21171 (PS9) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   eGFP (ORF1ab )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGWB502-omega

   Plasmid

   #74845

   Purpose

   Gateway cloning compatible binary vector for expression of gene by 2xCaMV35S enhancer and omega sequence.

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   2xCaMV35S enhancer and omega

   Available since

   Oct. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. EGFP-E-Syt2

   Plasmid

   #66831

   Purpose

   Mammalian Expression, Bacterial Expression

   Depositor

   Pietro De Camilli

   Article

   Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

   Insert

   E-Syt2 (ESYT2 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDIRECT_23C

    Plasmid

    #91140

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:bar

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCEP4-myc-HLA-B7

    Plasmid

    #135509

    Purpose

    Mammalian expression of myc-tagged HLA-B*07:02

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-B*07:02 (HLA-B Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-Tet-coBxb1-2A-BFP_IRES-iCasp9-2A-Blast_rtTA3

    Plasmid

    #171588

    Purpose

    Lentivector encoding a DNA recombinase landing pad with Bxb1 integrase and iCasp9 negative selection behind a Tet-inducible promoter

    Depositor

    Kenneth Matreyek

    Article

    Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

    Inserts

    Bxb1 integrase
    iCasp9
    BFP
    rtTA3G
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEF1a-mMLH1dn

    Plasmid

    #174825

    Purpose

    Mammalian expression of mouse MLH1dn (codon optimized)

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    mouse MLH1dn (codon optimized)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deletion of residues 758-760

    Promoter

    EF1a

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. CoV2-N-R203M

    Plasmid

    #177952

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (R203M). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (R203M) (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R203M

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. tdp43-EGFP construct4

    Plasmid

    #28197

    Depositor

    Zuoshang Xu

    Article

    Yang et al PLoS One. 2010 . 5(12):e15878.

    Insert

    Tar DNA binding protein (TARDBP Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa216-414 only

    Promoter

    Available since

    Sept. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lenti-dual-CRISPR-U6-H1(pBP43)

    Plasmid

    #218930

    Purpose

    The backbone lentiviral dual-CRISPR plasmid for making the dual-CRISPR screening libraries.

    Depositor

    Baoxu Pang

    Article

    Li et al Nat Biomed Eng. 2024 May 22. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6 and H1

    Available since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-Drp1K38A

    Plasmid

    #45161

    Depositor

    Richard Youle, Alexander van der Bliek

    Article

    Smirnova et al J Cell Biol. 1998 Oct 19;143(2):351-8.

    Insert

    Drp1 (DNM1L Human)
    

    Use

    Tags

    HA (AYPYDVPDY)

    Expression

    Mammalian

    Mutation

    K38A

    Promoter

    CMV

    Available since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAT15516_BEAR-GFP-2in1

    Plasmid

    #174082

    Purpose

    BEAR-GFP plasmid with a gRNA that targets its own plasmid

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    EGFP split with an intron between amino acids 95-96
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    disrupted 5' splice site

    Promoter

    CMV

    Available since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCRISPRx-Sth1Cas9-L5

    Plasmid

    #140993

    Purpose

    Sth1Cas9, TetR, KanR, L5Int, attP to create frameshifts and gene deletion in Mycobacteria

    Depositor

    Wilbert Bitter

    Article

    Meijers et al Tuberculosis (Edinb). 2020 Sep;124:10198

    Inserts

    Sth1Cas9
    TetR
    L5 attP
    L5 Int
    KanR
    

    Use

    Unspecified; Integrative in mycobactreia

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 1136- Desmoplakin-GFP

    Plasmid

    #32227

    Depositor

    Kathleen Green

    Article

    Godsel et al J Cell Biol. 2005 Dec 19;171(6):1045-59.

    Insert

    Desmoplakin (DSP Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDIRECT_21C

    Plasmid

    #91130

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:hpt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. lentiCRISPR-v2-HygR-EGFP

    Plasmid

    #167188

    Purpose

    Lentiviral expression of sgRNA with hygromycin resistance gene and EGFP

    Depositor

    Yin Shen

    Article

    Ren et al Sci Adv. 2021 Sep 17;7(38):eabi4360. doi

    Insert

    Hygromycin B phosphotransferase and EGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pQE-BR1

    Plasmid

    #160333

    Purpose

    This plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its quantum yield is improved compared to wild type.

    Depositor

    Sang Taek Jung

    Article

    Ko et al J Agric Food Chem. 2019 Oct 30;67(43):12

    Insert

    BR1
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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24. pcDNA3.1 His-NLRP1 codon-optimized

    Plasmid

    #166833

    Purpose

    Mammalian codon-optimized NLRP1 with N-terminal and C-terminal HIS tags

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    HIS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLentiPGK Hygro DEST ERKKTRmCerulean3

    Plasmid

    #90229

    Purpose

    Lentiviral vector to express ERK KTR mCerulean3 under PGK promoter (With Hygromycin Resistance)

    Depositor

    Markus Covert

    Article

    Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

    Insert

    ERK Kinase Translocation Reporter
    

    Use

    Lentiviral

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    March 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. gRNA NIA TLS2

    Plasmid

    #196981

    Purpose

    Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Each gRNA is fused to TLS2 mobility signal that confers graft mobility.

    Depositor

    Friedrich Kragler

    Article

    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    AtNIA1 gRNAs fused to TLS2 tags
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    pU6-26, pU6-29

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. gRNA NIA TLS1

    Plasmid

    #196980

    Purpose

    Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Each gRNA is fused to TLS1 mobility signal that confers graft mobility.

    Depositor

    Friedrich Kragler

    Article

    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    AtNIA1 gRNAs fused to TLS1 tags
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    pU6-26, pU6-29

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLV-pGK-Nluc-Fluc-NLS-P2A-H2B-mCherry-IRES-Hygro

    Plasmid

    #115684

    Purpose

    NanoLuc-Firefly Luciferase fusion with constitutive nuclear expression for calibration of Nanoluc signal to Firefly Luciferase signal. H2B-mCherry marks chromatin with red fluorescence.

    Depositor

    David Suter

    Article

    Mandic et al Methods. 2017 May 1;120:65-75. doi: 10.1

    Insert

    Nluc-Fluc-NLS-P2A-H2B-mCherry-IRES-Hygro
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. VRQR variant

    Plasmid

    #138562

    Purpose

    Expresses human codon-optimized SpCas9-VRQR and blasticidin resistance: EFS promoter-VRQR-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    VRQR
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    D1135V, G1218R, R1335Q, T1337R

    Promoter

    EFS

    Available since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMYC-GFP

    Plasmid

    #42142

    Depositor

    Olufunmilayo Olopade

    Article

    Chen et al BMC Cancer. 2011 Jun 13;11:246. doi: 10.

    Insert

    c-Myc (MYC Human)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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