We narrowed to 4,734 results for: Drosophila

1. pAc5.1B-lambdaN-HA-DmAGO1-L819Y-siRNAres_AC

   Plasmid

   #148394

   Purpose

   Insect Expression of DmAGO1-L819Y-siRNAres

   Depositor

   Elisa Izaurralde

   Article

   Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

   Insert

   DmAGO1-L819Y-siRNAres (AGO1 Fly)
   

   Expression

   Insect

   Mutation

   variation with 2 non silent mutations compared to…

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAc5.1B-lambdaN-HA-DmAGO1-P714GA744FL819Y-siRNAres_AC

   Plasmid

   #148395

   Purpose

   Insect Expression of DmAGO1-P714GA744FL819Y-siRNAres

   Depositor

   Elisa Izaurralde

   Article

   Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

   Insert

   DmAGO1-P714GA744FL819Y-siRNAres (AGO1 Fly)
   

   Expression

   Insect

   Mutation

   variation with 2 silent mutations compared to the…

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Donor_YTHDF2_KO_Neo

   Plasmid

   #186672

   Purpose

   Donor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. Neomycin and polyA sequence is inserted between two homology arms.

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   YTHDF2 KO Neomycin (YTHDF2 Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Donor_ANKRD1_KO_Hygro

   Plasmid

   #186668

   Purpose

   Donor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. eEF1a promoter, hygromycin and polyA sequence is inserted between two homology arms.

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   ANKRD1 KO Hygromycin (ANKRD1 Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Cas9_ANKRD1_sgRNA2

   Plasmid

   #186669

   Purpose

   Plasmid containing Cas9 and ANKRD1 sgRNA2 , sgRNA sequence: cggtcagcttatatagct.

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   ANKRD1 KO sgRNA Plasmid (ANKRD1 Human)
   

   Expression

   Mammalian

   Available Since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX-2T-CBP(1603-2678)

   Plasmid

   #183774

   Purpose

   Express GST-CBP(1603-2678) in E. coli. Purified GST-CBP(1603-2678) was used for in vitro acetylation assays.

   Depositor

   Peter Harte

   Article

   Tie et al Mol Cell Biol. 2012 Jun;32(12):2323-34.

   Insert

   nej (nej Fly)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX-2T-TRX-C751-E3616S

   Plasmid

   #182844

   Purpose

   mutation E3616S (GAA/TCA), PCR product coding C-terminal residues 2976-3726 of TRX was inserted into modified pGEX-2T by Nde I-Nsi I sites. Expression in E. coli. by IPTG induction

   Depositor

   Peter Harte

   Article

   Tie et al Development. 2014 Mar;141(5):1129-39. do

   Insert

   TRX (w Fly)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pnEA-NpM-DmThor_50-83-V75DL79D-GB1_AB

   Plasmid

   #148379

   Purpose

   Bacterial Expression of DmThor_50-83-V75DL79D

   Depositor

   Elisa Izaurralde

   Article

   Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

   Insert

   DmThor_50-83-V75DL79D (Thor Fly)
   

   Expression

   Bacterial

   Mutation

   two silent mutations compared to the sequence giv…

   Available Since

   March 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pnEA-NpM-DmThor_50-83-R56ER63E-GB1_AA

   Plasmid

   #148272

   Purpose

   Bacterial Expression of DmThor_50-83-R56ER63E

   Depositor

   Elisa Izaurralde

   Article

   Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

   Insert

   DmThor_50-83-R56ER63E (Thor Fly)
   

   Expression

   Bacterial

   Available Since

   March 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAc5.1C-Fluc-CG1856_A

    Plasmid

    #145905

    Purpose

    Insect Expression of CG1856-3UTR

    Depositor

    Elisa Izaurralde

    Article

    Eulalio et al RNA. 2009 Jan . 15(1):21-32.

    Insert

    CG1856-3UTR (ttk Fly)
    

    Expression

    Insect

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAW-BiP-Nb127D01-mCherry-KDEL

    Plasmid

    #171575

    Purpose

    Expresses mCherry-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-mCherry) in fly cell ER membrane

    Depositor

    Hidde Ploegh, Norbert Perrimon

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    BiP-Nb127D01-mCherry-KDEL (CXCR2 Human)
    

    Tags

    BiP signal peptide and mCherry-KDEL

    Expression

    Insect

    Promoter

    fly actin5C promoter

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAW-BiP-NbVHH05-mCherry-KDEL

    Plasmid

    #171574

    Purpose

    Expresses mCherry-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-mCherry) in fly cell ER membrane

    Depositor

    Hidde Ploegh, Norbert Perrimon

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    BiP-NbVHH05-mCherry-KDEL (UBE2J1 Human)
    

    Tags

    BiP signal peptide and mCherry-KDEL

    Expression

    Insect

    Promoter

    fly actin5C promoter

    Available Since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. OA-1045D

    Plasmid

    #125006

    Purpose

    Expresses gRNAs targeting hedgehog and wingless

    Depositor

    Omar Akbari

    Article

    Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

    Insert

    wg gRNA (wg Fly)
    

    Expression

    Insect

    Promoter

    u6:3

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFOSGE_COF4D03

    Plasmid

    #70969

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    Taf12 (Taf12 Fly)
    

    Use

    Gateway entry vector

    Available Since

    Dec. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pFOSGE_CUS1F03

    Plasmid

    #71071

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    Su(var)205 (Su(var)205 Fly)
    

    Use

    Gateway entry vector

    Available Since

    Nov. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pMT-Ex

    Plasmid

    #231106

    Purpose

    Inducible protein expression in insect cell culture. Stable cell line generation.

    Depositor

    Michael Jeltsch

    Type

    Empty backbone

    Tags

    6xHis and V5

    Expression

    Insect

    Promoter

    Drosophila metallothionein promoter, MT

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pIVTR(T7A)-Cas9n-RT-T2A-PuroR

    Plasmid

    #172296

    Purpose

    In vitro transcription of Cas9n-RT-T2A-PuroR synthetic construct for PRIME editing

    Depositor

    Patrik Verstreken

    Insert

    Cas9n-RT-T2A-PuroR
    

    Use

    CRISPR

    Mutation

    H840A (Cas9)

    Promoter

    T7 promoter A-initiating

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. H3K9me3 biosensor

    Plasmid

    #120802

    Purpose

    FRET biosensor. To monitor histone H3 lysine 9 tri-methylation in mammalian cells by FRET

    Depositor

    Yingxiao Wang

    Article

    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

    Insert

    Truncated YPet-HP1-EV linker-ECFP-mouse histone H3
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. W45A mutant of H3K9me3 biosensor

    Plasmid

    #120808

    Purpose

    FRET biosensor. Disrupting HP1 binding capability in H3K9me3 biosensor to abolish the detection capability

    Depositor

    Yingxiao Wang

    Article

    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

    Insert

    Truncated YPet-HP1(W45A mutation)-EV linker-ECFP-mouse histone H3
    

    Expression

    Mammalian

    Mutation

    Tryptophan 45 is mutated to Alanine 45 on HP1 dom…

    Promoter

    CMV

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. K9L mutant of H3K9me3 biosensor

    Plasmid

    #120807

    Purpose

    FRET biosensor. Eliminating the H3 lysine 9 methylation site in H3K9me3 biosensor to abolish the detection capability

    Depositor

    Yingxiao Wang

    Article

    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

    Insert

    Truncated YPet-HP1-EV linker-ECFP-mouse histone H3(K9L mutation)
    

    Expression

    Mammalian

    Mutation

    lysine 9 is mutated to leucine 9 on histone H3

    Promoter

    CMV

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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