The interactions of GW182 proteins with PABP and deadenylases are required for both translational repression and degradation of miRNA targets.
Huntzinger E, Kuzuoglu-Ozturk D, Braun JE, Eulalio A, Wohlbold L, Izaurralde E
Nucleic Acids Res. 2013 Jan;41(2):978-94. doi: 10.1093/nar/gks1078. Epub 2012 Nov 21. PubMed Article
Huntzinger E, Kuzuoglu-Ozturk D, Braun JE, Eulalio A, Wohlbold L, Izaurralde E
Nucleic Acids Res. 2013 Jan;41(2):978-94. doi: 10.1093/nar/gks1078. Epub 2012 Nov 21. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 146709 | pAc5.1B-EGFP-DmNot1_J | Insect Expression of DmNot1 |
| 146710 | pAc5.1B-lambdaN-HA-DmNot1_J | Insect Expression of DmNot1 |
| 146889 | pCIneo-lambdaN-HA-HsNot7_L | Mammalian Expression of HsNot7 |
| 146890 | pT7-EGFP-C1-HsNot7-D40AE42A_L | Mammalian Expression of HsNot7-D40AE42A. Please note that this plasmid does not contain the T7 promoter. |
| 147057 | pAc5.1B-EGFP-DmGW182_1-603_N | Insect Expression of DmGW182_1-603 |
| 147058 | pAc5.1B-EGFP-DmGW182_1-634_N | Insect Expression of DmGW182_1-634 |
| 147059 | pAc5.1B-EGFP-DmGW182_1-824_N | Insect Expression of DmGW182_1-824 |
| 147060 | pAc5.1B-EGFP-DmGW182_604-1115_N | Insect Expression of DmGW182_604-1115 |
| 147061 | pAc5.1B-EGFP-DmGW182_604-1384_N | Insect Expression of DmGW182_604-1384 |
| 147062 | pAc5.1B-EGFP-DmGW182_635-1384_N | Insect Expression of DmGW182_635-1384 |
| 147063 | pAc5.1B-EGFP-DmGW182_825-1384_N | Insect Expression of DmGW182_825-1384 |
| 147064 | pETM-41P-DmGW182_744-817_N | Bacterial Expression of DmGW182_744-817 |
| 147150 | pAc5.1B-EGFP-DmGW182_539-830_O | Insect Expression of DmGW182_539-830 |
| 147151 | pAc5.1B-EGFP-DmGW182_539-1115_O | Insect Expression of DmGW182_539-1115 |
| 147152 | pAc5.1B-EGFP-DmGW182_539-1384-dsRNAres_O | Insect Expression of DmGW182_539-1384-dsRNAres |
| 147153 | pAc5.1B-EGFP-DmGW182-del539-830-dsRNAres_O | Insect Expression of DmGW182-del539-830-dsRNAres |
| 147154 | pAc5.1B-EGFP-DmGW182-Nterm6Wmut-SD6Wmut_O | Insect Expression of DmGW182-Nterm6Wmut-SD6mut |
| 147155 | pAc5.1B-EGFP-DmGW182-SD6Wmut_O | Insect Expression of DmGW182-SD6Wmut |
| 147156 | pAc5.1B-EGFP-SbfI_O | Insect Expression vector |
| 147157 | pAc5.1B-lambdaN-HA-DmGW182-12xmut_1-539_O | Insect Expression of DmGW182-12xmut_1-539 |
| 147341 | pAc5.1B-EGFP-DmNot2-dsRNAres_Q | Insect Expression of DmNot2-dsRNAres |
| 147342 | pAc5.1B-lambdaN-HA-DmNot2-dsRNAres_Q | Insect Expression of DmNot2-dsRNAres |
| 147382 | pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_1-634-dsRNAres_R | Insect Expression of CeAIN2_254-400-DmGW182_1-634-dsRNAres |
| 147383 | pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_205-490-dsRNAres_R | Insect Expression of CeAIN2_254-400-DmGW182_205-490-dsRNAres |
| 147384 | pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384_R | Insect Expression of CeAIN2_254-400-DmGW182_635-1384 |
| 147385 | pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384-CIM1-mut_R | Insect Expression of CeAIN2_254-400-DmGW182_635-1384-CIM1-mut |
| 147386 | pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384-F961A_R | Insect Expression of CeAIN2_254-400-DmGW182_635-1384-F961A |
| 147387 | pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384-M2WA_R | Insect Expression of CeAIN2_254-400-DmGW182_635-1384-M2WA |
| 147388 | pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384-M2WA-CIM1mut_R | Insect Expression of CeAIN2_254-400-DmGW182_635-1384-M2WA-CIM1mut |
| 147389 | pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384-M2WA-CIM1mut-F961A_R | Insect Expression of CeAIN2_254-400-DmGW182_635-1384-M2WA-CIM1mut-F961A |
| 147390 | pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384-P-GLmut_R | Insect Expression of CeAIN2_254-400-DmGW182_635-1384-P-GLmut |
| 147391 | pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384-P-GLmut-F961A_R | Insect Expression of CeAIN2_254-400-DmGW182_635-1384-P-GLmut-F961A |
| 147392 | pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_862-1384_R | Insect Expression of CeAIN2_254-400-DmGW182_862-1384 |
| 147393 | pAc5.1B-EGFP-CeAIN2_254-400-HsTNRC6C_1207-1690_R | Insect Expression of CeAIN2_254-400-HsTNRC6C_1207-1690 |
| 147394 | pAc5.1B-EGFP-CeAIN2_254-400-HsTNRC6C_1207-1690-CIM1-2mut-F1389A_R | Insect Expression of CeAIN2_254-400-HsTNRC6C_1207-1690-CIM1-2mut-F1389A |
| 147395 | pAc5.1B-EGFP-CeAIN2_254-400-HsTNRC6C_1207-1690-CIM1mut_R | Insect Expression of CeAIN2_254-400-HsTNRC6C_1207-1690-CIM1mut |
| 147396 | pAc5.1B-EGFP-CeAIN2_254-400-HsTNRC6C_1207-1690-CIM1mut-CIM2mut_R | Insect Expression of CeAIN2_254-400-HsTNRC6C_1207-1690-CIM1-2mut |
| 147397 | pAc5.1B-EGFP-CeAIN2_254-400-HsTNRC6C_1207-1690-CIM2mut_R | Insect Expression of CeAIN2_254-400-HsTNRC6C_1207-1690-CIM2mut |
| 147398 | pAc5.1B-EGFP-CeAIN2_254-400-HsTNRC6C_1207-1690-F1389A_R | Insect Expression of CeAIN2_254-400-HsTNRC6C_1207-1690-F1389A |
| 147399 | pAc5.1B-EGFP-CeAIN2_254-400-HsTNRC6C_1207-1690-P-GL-mut_R | Insect Expression of CeAIN2_254-400-HsTNRC6C_1207-1690-P-GL-mut |
| 147400 | pAc5.1B-EGFP-CeAIN2_254-400-V5His6_R | Insect Expression of CeAIN2_254-400 |
| 147401 | pAc5.1B-EGFP-DmGW182_1-861-dsRNAres_R | Insect Expression of DmGW182_1-861-dsRNAres |
| 147402 | pAc5.1B-EGFP-DmGW182-del205-490-dsRNAres_R | Insect Expression of DmGW182-del205-490-dsRNAres |
| 147403 | pAc5.1B-lambdaN-DmGW182-del205-490-dsRNAres_R | Insect Expression of DmGW182-del205-490-dsRNAres |
| 147404 | pAc5.1B-lambdaN-HA-CeAIN2_254-400-DmGW182_635-861_R | Insect Expression of CeAIN2_254-400-DmGW182_635-861 |
| 147405 | pAc5.1B-lambdaN-HA-CeAIN2_254-400-DmGW182_635-1384_R | Insect Expression of CeAIN2_254-400-DmGW182_635-1384 |
| 147406 | pAc5.1B-lambdaN-HA-CeAIN2_254-400-DmGW182_862-1384_R | Insect Expression of CeAIN2_254-400-DmGW182_862-1384 |
| 147407 | pAc5.1B-lambdaN-HA-CeAIN2_254-400-V5His6_R | Insect Expression of CeAIN2_254-400 |
| 147408 | pAc5.1B-lambdaN-HA-DmGW182_1-861-dsRNAres_R | Insect Expression of DmGW182_1-861-dsRNAres |
| 147409 | pAc5.1B-lambdaN-HA-DmGW182-del205-490-dsRNAres_R | Insect Expression of DmGW182-del205-490-dsRNAres |
| 147410 | pAc5.1B-lambdaN-HA-HsTNRC6C_1207-1609_R | Insect Expression of HsTNRC6C_1207-1609 |
| 147411 | pCIneo-lambdaN-HA-HsTNRC6A-CIM1-2mut-F1359A-siRNAres_R | Insect Expression of HsTNRC6A-CIM1-2mut-F1359A-siRNAres |
| 147412 | pCIneo-lambdaN-HA-HsTNRC6A-CIM1-2mut-siRNAres_R | Insect Expression of HsTNRC6A-CIM1-2mut-siRNAres |
| 147413 | pCIneo-lambdaN-HA-HsTNRC6A-CIM1mut-siRNAres_R | Mammalian Expression of HsTNRC6A-CIM1mut-siRNAres |
| 147414 | pCIneo-lambdaN-HA-HsTNRC6A-CIM2mut-siRNAres_R | Insect Expression of HsTNRC6A-CIM2mut-siRNAres |
| 147512 | pGEX-6P-1-HsTNRC6A_1209-1709-M2WA-CIM1-2mut-F1359A_S | Bacterial Expression of HsTNRC6A_1209-1709-M2WA-CIM1-2mut-F1359A |
| 147513 | pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_205-830-dsRNAres_S | Insect Expression of CeAIN2_254-400-DmGW182_205-830-dsRNAres |
| 147514 | pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384-CIM1mut-F961A_S | Insect Expression of CeAIN2_254-400-DmGW182_635-1384-CIM1-F961A |
| 147515 | pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384-CIM1-P-GLmut-F961A_S | Insect Expression of CeAIN2_254-400-DmGW182_635-1384-CIM1-P-GLmut-F961A |
| 147516 | pCIneo-lambdaN-HA-HsTNRC6A-M2WA-CIM1-2mut-F1359A-siRNAres_S | Insect Expression of HsTNRC6A-M2WA-CIM1-2mut-F1359A-siRNAres |
| 147517 | pCIneo-lambdaN-HA-HsTNRC6A-M2WA-CIM1-2mut-siRNAres_S | Insect Expression of HsTNRC6A-M2WA-CIM1-2mut-siRNAres |
| 147518 | pCIneo-lambdaN-HA-HsTNRC6A-M2WA-F1359A-siRNAres_S | Mammalian Expression of HsTNRC6A-M2WA-F1359A-siRNAres |
| 147519 | pCIneo-lambdaN-HA-HsTNRC6A-M2WA-siRNAres_S | Mammalian Expression of HsTNRC6A-M2WA-siRNAres |
| 147520 | pGEX-6P-1-HsTNRC6A_1209-1709_S | Bacterial Expression of HsTNRC6A_1209-1709 |
| 147521 | pGEX-6P-1-HsTNRC6A_1209-1709-CIM1-2mut_S | Bacterial Expression of HsTNRC6A_1209-1709-CIM1-2mut |
| 147522 | pGEX-6P-1-HsTNRC6A_1209-1709-CIM1-2mut-F1359A_S | Bacterial Expression of HsTNRC6A_1209-1709-CIM1-2mut-F1359A |
| 147523 | pGEX-6P-1-HsTNRC6A_1209-1709-CIM1mut_S | Bacterial Expression of HsTNRC6A_1209-1709-CIM1mut |
| 147524 | pGEX-6P-1-HsTNRC6A_1209-1709-CIM2mut_S | Bacterial Expression of HsTNRC6A_1209-1709-CIM2mut |
| 147525 | pGEX-6P-1-HsTNRC6A_1209-1709-F1359A_S | Bacterial Expression of HsTNRC6A_1209-1709-F1359A |
| 147526 | pGEX-6P-1-HsTNRC6A_1209-1709-M2WA_S | Bacterial Expression of HsTNRC6A_1209-1709-M2WA |
| 147527 | pGEX-6P-1-HsTNRC6A_1209-1709-M2WA-CIM1-2mut_S | Bacterial Expression of HsTNRC6A_1209-1709-M2W1-CIM1-2mut |
| 147528 | pGEX-6P-1-HsTNRC6A_1209-1709-M2WA-F1359A_S | Bacterial Expression of HsTNRC6A_1209-1709-M2W1-F1359A |
| 147634 | pAc5.1B-lambdaN-HA-HsTNRC6C_1207-1609-M2WA_T | Insect Expression of HsTNRC6C_1207-1609-M2WA |
| 147635 | pAc5.1B-lambdaN-HA-HsTNRC6C_1207-1609-M2WA-CIM1-2mut_T | Insect Expression of HsTNRC6C_1207-1609-M2WA-CIM1-2mut |
| 147636 | pAc5.1B-lambdaN-HA-HsTNRC6C_1207-1609-M2WA-CIM1-2mut-F1389A_T | Insect Expression of HsTNRC6C_1207-1609-M2WA-CIM1-2mut-F1389A |