We narrowed to 13,642 results for: NTS

1. ZIKV_Sensor_27B_LacZ

   Plasmid

   #75006

   Purpose

   Toehold switch sensor to detect Zika Virus

   Depositor

   Alexander Green, James Collins

   Article

   Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

   Insert

   ZIKV_sensor_27B_LacZ
   

   Use

   Tags

   LacZ

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. xCas9(3.7)-ABE(7.10)

   Plasmid

   #108382

   Purpose

   Mammalian expression vector for xCas9(3.7)-ABE

   Depositor

   David Liu

   Article

   Hu et al Nature. 2018 Feb 28. pii: nature26155. d

   Insert

   xCas9(3.7)-ABE(7.10)
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   A262T, R324L, S409I, E480K, E543D, M694I, E1219V

   Promoter

   Available since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDGB3 alpha1 P35S:LbCas12a:T35S (GB1441)

   Plasmid

   #160611

   Purpose

   Transcriptional unit for the expression of the LbCas12a in plant systems driven by the CaMV35s promoter.

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

   Insert

   P35s:LbCas12a:T35s
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   P35S

   Available since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQ-Cas9-Act3.0

   Plasmid

   #178954

   Purpose

   It consists of a catalytically active Cas9 nuclease and MS2-SunTag-activators activation complex (ScFv-sfGFP-2xTAD), enabling simultaneous genome editing and gene activation.

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

   Insert

   zCas9-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAG496

   Plasmid

   #226750

   Purpose

   Expresses exogenous MTCH2

   Depositor

   Jonathan Weissman

   Article

   Guna et al Science. 2022 Oct 21;378(6617):317-322.

   Insert

   MTCH2 (MTCH2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCEP4-FLAG-TAPBPR

   Plasmid

   #135500

   Purpose

   Mammalian expression of FLAG-tagged TAPBPR

   Depositor

   Erik Procko

   Article

   McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

   Insert

   TAPBPR (TAPBPL Human)
   

   Use

   Tags

   Luminal FLAG epitope tag and Signal peptide from …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pmScarlet-HSP70

   Plasmid

   #163790

   Purpose

   Expresses mScarlet-Hsp70 in mammalian cells

   Depositor

   Vincent Timmerman

   Article

   Adriaenssens et al Sci Rep. 2020 May 29;10(1):8755. doi: 10

   Insert

   HSP70 (HSPA1A Human)
   

   Use

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBUE411

   Plasmid

   #62200

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Inserts

   gRNA scaffold
   zCas9
   

   Use

   Plant binary vector

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   maize codon optimized

   Promoter

   OsU3 and Ubi

   Available since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. CoV2-N-WT-Hu1

   Plasmid

   #177937

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Nucleocapsid (original Wuhan Hu1 sequence) (N )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPPED

    Plasmid

    #162468

    Purpose

    Destination Expression vector for dicot plant prime editing

    Depositor

    Adam Bogdanove

    Article

    Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do

    Inserts

    Cas9H840A
    attR gateway destination cassette
    

    Use

    Tags

    MLV reverse transcriptase

    Expression

    Plant

    Mutation

    Change histidine 840 to Alanine based sequence o…

    Promoter

    2x35S

    Available since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-ABEmax-NRTH

    Plasmid

    #136922

    Purpose

    Expresses ABEmax-NRTH in mammalian cells

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    ABEmax-NRTH
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    see manuscript

    Promoter

    CMV

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMOD_B2303

    Plasmid

    #91068

    Purpose

    Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35S

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    CmYLCV

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. p11d-aRPA (MSW#712)

    Plasmid

    #208078

    Purpose

    Expression of Alternative human Replication Protein A (RPA1,4,3) in E. coli

    Depositor

    Marc Wold

    Article

    Mason et al J Biol Chem. 2009 Feb 20;284(8):5324-31.

    Insert

    RPA1, RPA4, RPA3 (RPA1, RPA3, RPA4 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. CoV2-N-T205I

    Plasmid

    #177954

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (T205I). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (T205I) (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T205I

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. AY27_pU6.gRNA.CLYBL

    Plasmid

    #199238

    Purpose

    Expression construct encoding a S. pyogenes guide RNA targeting the human CLYBL safe harbor locus

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

    Insert

    S. pyogenes gRNA spacer
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RNA polymerase III promoter for human U6 snRNA

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-CAG-DIO-EYFP

    Plasmid

    #104052

    Purpose

    An AAV genome encoding Cre-dependent expression of the fluorescent protein EYFP from the CAG promoter

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Has Service

    AAV PHP.V1

    Insert

    EYFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Jan. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. SpCas9-NG

    Plasmid

    #138566

    Purpose

    Expresses human codon-optimized rationally engineered SpCas9 variant and blasticidin resistance: EFS promoter-SpCas9-NG-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    SpCas9-NG
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T…

    Promoter

    EFS

    Available since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. ICMAB - pET22b T7 EncFtn Mms6

    Plasmid

    #225538

    Purpose

    Construction of intracellular magnetite accumulating bacterial systems.

    Depositor

    Urartu Seker

    Article

    Yavuz et al Small. 2022 Jul;18(26):e2200537. doi: 10

    Inserts

    Iron binding protein
    Ferroxidase
    Material binding peptite
    Iron transporter protein
    

    Use

    Tags

    no

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CoV2-N-P199L

    Plasmid

    #177949

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P199L). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (P199L) (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    P199L

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-ABEmax-NRCH

    Plasmid

    #136923

    Purpose

    Expresses ABEmax-NRCH in mammalian cells

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    ABEmax-NRCH
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    see manuscript

    Promoter

    CMV

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. CoV2-N-S202R

    Plasmid

    #177950

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (S202R). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (S202R) (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S202R

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLYS6-METTL17-FLAG

    Plasmid

    #220875

    Purpose

    Expressing METTL17-FLAG

    Depositor

    Vamsi Mootha

    Article

    Ast et al Mol Cell. 2024 Jan 18;84(2):359-374.e8.

    Insert

    METTL17 (METTL17 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHUE411

    Plasmid

    #62203

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Hyg resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    gRNA scaffold
    zCas9
    

    Use

    Plant binary vector

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    maize codon optimized

    Promoter

    OsU3 and Ubi

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. tdp43-EGFP construct3

    Plasmid

    #28196

    Depositor

    Zuoshang Xu

    Article

    Yang et al PLoS One. 2010 . 5(12):e15878.

    Insert

    Tar DNA binding protein (TARDBP Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa170-414 only

    Promoter

    Available since

    Sept. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

25. pCPH2A.X-GS3

    Plasmid

    #204745

    Purpose

    Expression of Cas9 and human H2AX

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

    Insert

    H2AX (H2AX Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. p35S_GFP-GUS

    Plasmid

    #167122

    Purpose

    Plant binary expression vector containing mGFP5-GUS fusion reporter under CaMV 35S promoter.

    Depositor

    Olivier Voinnet, Arturo Marí-Ordóñez

    Article

    Oberlin et al Genome Res. 2017 Sep;27(9):1549-1562. do

    Insert

    GFP-GUS
    

    Use

    Tags

    mGFP5, GUS

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBT2-4xUAS:P2A-mCerulean3

    Plasmid

    #127592

    Purpose

    This vector is used for cloning a gene driven by 4xnrUAS, to be co-expressed with mCerulean3 through P2A self-cleaving peptides.

    Depositor

    Longhou Fang

    Article

    Gu et al Science. 2019 Mar 8;363(6431):1085-1088.

    Type

    Empty backbone

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-hSyn-GRAB_OT1.0

    Plasmid

    #185386

    Purpose

    Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in neurons

    Depositor

    Yulong Li

    Article

    Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Has Service

    AAV1 and AAV9

    Insert

    GPCR activation based oxytocin sensor GRAB_OT1.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Sept. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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29. pLX302 OASL-V5 puro

    Plasmid

    #158643

    Purpose

    Lentiviral expression vector for constitutive expression of V5-tagged OASL.

    Depositor

    Kevin Janes

    Article

    Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

    Insert

    OASL (OASL Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEF1a-hMLH1NTD-NLS

    Plasmid

    #174826

    Purpose

    Mammalian expression of human MLH1NTD-NLS (codon optimized)

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    human MLH1_NTD-NLS (codon optimized)
    

    Use

    Tags

    SV40 bpNLS

    Expression

    Mammalian

    Mutation

    truncation of residues 1-335

    Promoter

    EF1a

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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