We narrowed to 5,315 results for: pcdna3.1

1. NSP6-FR-2Strep

   Plasmid

   #210317

   Purpose

   Expresses SARS-CoV-2 NSP6 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   SARS-CoV-2 NSP6 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Tags

   2Strep

   Expression

   Mammalian

   Mutation

   Many synonymous changes due to codon optimization

   Promoter

   Available Since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. ITSN-mcherry-sspB

   Plasmid

   #85220

   Purpose

   Role of Cdc-42 selective GEF domain from ITSN in immune cell migration

   Depositor

   Narasimhan Gautam

   Article

   O'Neill et al Mol Biol Cell. 2016 May 1;27(9):1442-50.

   Inserts

   ITSN1(1225-1575) (ITSN1 Human)
   mcherry
   sspB
   

   Use

   Tags

   NA

   Expression

   Mammalian

   Mutation

   R73Q

   Promoter

   CMV

   Available Since

   Jan. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRD405

   Plasmid

   #163110

   Purpose

   Expression of mScarlet-I_H-NSdbd in eukaryotes for visualisation of nuclear DNA

   Depositor

   Remus Dame

   Article

   Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

   Insert

   NLS_mScarlet-I_H-NSdbd_NLS
   

   Use

   Eukaryotic expression

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter; T7 promoter

   Available Since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCDNA3- SREBP-1

   Plasmid

   #204192

   Purpose

   Mammalian expression of SREBP-1 (isoform 1)

   Depositor

   Allan Weissman

   Article

   Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

   Insert

   SREBP-1 (SREBF1 Human)
   

   Use

   Tags

   E-tag and T7x2

   Expression

   Mammalian

   Mutation

   multiple silent mutations

   Promoter

   Available Since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV-Target-AIDmax

   Plasmid

   #139107

   Purpose

   Mammalian Target-AIDmax expression vector (pSI-626)

   Depositor

   Nozomu Yachie

   Article

   Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   Bi_partite_NLS-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available Since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRD406

   Plasmid

   #163111

   Purpose

   Expression of mNeonGreen_H-NSdbd in eukaryotes for visualisation of nuclear DNA

   Depositor

   Remus Dame

   Article

   Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

   Insert

   NLS_mNeonGreen_H-NSdbd_NLS
   

   Use

   Eukaryotic expression

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter; T7 promoter

   Available Since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRD399

   Plasmid

   #163106

   Purpose

   Expression of mEGFP_H-NSdbd in eukaryotes for visualisation of nuclear DNA

   Depositor

   Remus Dame

   Article

   Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

   Insert

   NLS_mEGFP_H-NSdbd_NLS
   

   Use

   Eukaryotic expression

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter; T7 promoter

   Available Since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. SacI ML GFP Strand 11 Long

   Plasmid

   #164120

   Purpose

   Detect the long Mitochondria-Endoplasmic reticulum contact along with the OMMGFP1-10

   Depositor

   Tito Calì, Marisa Brini

   Article

   Cieri et al Cell Death Differ. 2018 Jun;25(6):1131-1

   Insert

   SacI ML GFP Strand 11 Long
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-sACE2(WT)-8his

   Plasmid

   #149268

   Purpose

   Mammalian expression plasmid for his-tagged sACE2

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Use

   Tags

   Short GSG linker and 8his tag

   Expression

   Mammalian

   Mutation

   Extracellular, soluble protease domain (a.a. M1-D…

   Promoter

   CMV

   Available Since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GANLS

    Plasmid

    #50852

    Purpose

    Expresses one ddFP in mammalian cells, the second plasmid for a red to green colour switch-based fluorescent caspase-3 biosensor, used together with plasmid NESRA-DEVD-BNLS.

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddGFP A
    

    Use

    Tags

    triplicated NLS sequence DPKKKRKV

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

11. RANLS

    Plasmid

    #50843

    Purpose

    Expresses one ddFP in mammalian cells, the second plasmid for a green to red colour switch-based fluorescent caspase-3 biosensor, used together with plasmid GANES-DEVD-BNLS.

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddRFP A
    

    Use

    Tags

    triplicated NLS sequence DPKKKRKV

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. VN-Sph1

    Plasmid

    #89755

    Purpose

    expresses human Synphilin fused to the N-terminal part of Venus

    Depositor

    Tiago Outeiro

    Article

    Dominguez-Meijide et al Neurobiol Dis. 2021 Apr;151:105256. doi:

    Insert

    SNCAIP (SNCAIP Human)
    

    Use

    Tags

    N-terminal part of Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pY30

    Plasmid

    #84745

    Purpose

    Expresses huAsCpf1-P2A-puro and crRNA guide

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

    Inserts

    huAsCpf1
    puromycin resistance gene
    

    Use

    Tags

    3xHA, NLS, and T2A

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCDNA_MET_D14_3Flag

    Plasmid

    #182495

    Purpose

    Contains cDNA of the MET gene without the exon 14

    Depositor

    Paolo Comoglio

    Article

    Cerqua et al Life Sci Alliance. 2022 May 30;5(10). pi

    Insert

    MET (MET Human)
    

    Use

    Tags

    C-terminal 3xFlag tag

    Expression

    Mammalian

    Mutation

    Exon 14 deleted

    Promoter

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. ER-mGreenLantern

    Plasmid

    #164463

    Purpose

    Localization and retention of mGreenLantern fluorescent protein in the ER

    Depositor

    Gregory Petsko

    Article

    Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    mGreenLantern
    

    Use

    Tags

    Calreticulin and KDEL

    Expression

    Mammalian

    Mutation

    Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

    Promoter

    CMV

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA 3.1 LIC 6A NLRP1-FLAG

    Plasmid

    #166803

    Purpose

    Mammalian cell expression vector for FLAG-tagged NLRP1.

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. FLAG-MED22

    Plasmid

    #15423

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

    Insert

    MED22 (MED22 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

18. HiBiT-FZD4

    Plasmid

    #195842

    Purpose

    Expresses human FZD4 with an N-terminal HiBiT

    Depositor

    Gunnar Schulte

    Article

    Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

    Insert

    FZD4 (FZD4 Human)
    

    Use

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HiBiT-FZD2

    Plasmid

    #195840

    Purpose

    Expresses human FZD2 with an N-terminal HiBiT

    Depositor

    Gunnar Schulte

    Article

    Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

    Insert

    FZD2 (FZD2 Human)
    

    Use

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SacI ML GFP Strand 11 Short

    Plasmid

    #164121

    Purpose

    Detect the short Mitochondria-Endoplasmic reticulum contact along with the OMMGFP1-10

    Depositor

    Tito Calì, Marisa Brini

    Article

    Cieri et al Cell Death Differ. 2018 Jun;25(6):1131-1

    Insert

    SacI ML GFP Strand 11 Short
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge