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pLentiCRISPRv2-dADAM17 (#1)

Plasmid

#177259

Purpose

A knockout vector for the dog Adam17

Depositor

Michiyuki Matsuda

Publication

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Adam17 gene and the cDNA of CRISPR-Cas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV9-hSyn iCyt_BrEt_SnFR_PM

Plasmid

#177757

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Publication

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

pAAV9-hSyn iCyt_BrEt_SnFR_PM

Use

AAV

Promoter

hSyn

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_NR2E3_Hormone-recep

Plasmid

#109852

Purpose

Protein expression and purification of NR2E3_Hormone-recep

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

NR2E3_Hormone-recep (NR2E3 Human)

Expression

Bacterial

Available since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pFE-vcDAB

Plasmid

#133004

Purpose

pFE carrying the vcdabA2 ( locus_tag: CSW01_RS07960) and vcdabB2 genes (locus_tag: CSW01_RS07955)

Depositor

David Savage

Publication

Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

Inserts

vcdabB
vcdabA

Expression

Bacterial

Promoter

tetR

Available since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI166

Plasmid

#126075

Purpose

Vector for expression of mitochondria targeted improved YFP1-10 (T65G, T205S, T203Y) in plant cell

Depositor

Ralf-Bernd Klösgen

Publication

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

mitochondria Rieske iron sulphur protein (1-100 aa)

Use

Binary

Tags

YFP1-10 (improved)

Expression

Plant

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI128

Plasmid

#126209

Purpose

Module 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP11x3 and expression in plant cell

Depositor

Ralf-Bernd Klösgen

Publication

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Inserts

ccdB Cassette (BsaI)
GFP11x3 (5 aa linker)

Use

Binary, module 1

Expression

Plant

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pISH-Pde4a

Plasmid

#105463

Purpose

to generate a riboprobe for in situ hybridazation

Depositor

Peter Mombaerts

Publication

Omura et al Mol Cell Neurosci. 2015 Mar;65:114-24. d

Insert

Pde4a (Pde4a Mouse)

Use

To ganerate riboprobe

Available since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-miniSOG-Display-5

Plasmid

#54802

Purpose

Localization: Cytoplasm, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Publication

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Type

Empty backbone

Tags

mCherry-miniSOG-Display

Expression

Mammalian

Available since

July 10, 2014

Availability

Academic Institutions and Nonprofits only
pGEM-T ZfHoxd9a

Plasmid

#27535

Depositor

Victoria Prince

Publication

Prince et al Development. 1998 Feb . 125(3):407-20.

Insert

Hoxd9a (hoxd9a Zebrafish)

Expression

Bacterial

Available since

Feb. 16, 2011

Availability

Academic Institutions and Nonprofits only
pACT-HAB1_star

Plasmid

#241277

Purpose

Orthogonal PYR1/HAB1 sensor module

Depositor

Sean Cutler

Publication

Park et al Nat Chem Biol. 2024 Jan;20(1):103-110. d

Insert

HAB1_star (HAB1 Mustard Weed)

Expression

Yeast

Mutation

NA

Available since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_H2B-dHaloTag7_T2A_mEGFP

Plasmid

#187071

Purpose

Nuclear expression of dHaloTag7 in mammalian cells

Depositor

Kai Johnsson

Publication

Kompa et al J Am Chem Soc. 2023 Feb 8;145(5):3075-30

Insert

H2B_dHaloTag7

Expression

Mammalian

Mutation

dHaloTag7 = HaloTag7-D106A

Available since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pWal10-roe-sfGFP

Plasmid

#240239

Purpose

Gateway destination plasmid to generate C-terminal sfGFP fusions under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

Depositor

Justin Bosch, Norbert Perrimon

Publication

Bosch et al Nat Commun. 2026 Apr 20. doi: 10.1038/s4

Type

Empty backbone

Use

Gateway Cloning

Expression

Insect

Available since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-5102HRasCT(Picchu)

Plasmid

#209917

Purpose

A FRET biosensor for tyrosine kinase activity

Depositor

Michiyuki Matsuda

Publication

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

5102HRasCT

Tags

YPet and nTurquoise-GL

Expression

Mammalian

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO-perox-mCer

Plasmid

#185603

Purpose

Lentiviral construct for expression of mCerulean targeted to peroxisomes

Depositor

Jarrod Johnson

Publication

Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

Insert

perox-mCer

Use

Lentiviral

Expression

Mammalian

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP_I392N-talin1 (1-2541)

Plasmid

#166114

Purpose

expresses talin-1-I392N in mammalian cells

Depositor

Vesa Hytönen

Publication

Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

Insert

I392N-Talin-1 (Tln1 Mouse)

Use

Cmv promoter

Tags

EGFP

Expression

Mammalian

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_CASP2_Peptidase-C14

Plasmid

#109996

Purpose

Protein expression and purification of CASP2_Peptidase-C14

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

CASP2_Peptidase-C14 (CASP2 Human)

Expression

Bacterial

Available since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_ESRRG_Hormone-recep

Plasmid

#109910

Purpose

Protein expression and purification of ESRRG_Hormone-recep

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

ESRRG_Hormone-recep (ESRRG Human)

Expression

Bacterial

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_RANBP2_Ran-BP3

Plasmid

#109777

Purpose

Protein expression and purification of RANBP2_Ran-BP3

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

RANBP2_Ran-BP3 (RANBP2 Human)

Expression

Bacterial

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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ZJOM8

Plasmid

#133671

Purpose

Integration of SNF7-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Present on endosomes and vacuolar surface.

Depositor

Zhiping Xie

Publication

Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

Insert

p1k-SNF7-GFP (SNF7 Budding Yeast)

Tags

EGFP

Expression

Yeast

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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ath5:GFP-DCX

Plasmid

#105959

Purpose

transgenesis, doublecortin DCX

Depositor

Caren Norden

Publication

Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

Insert

DCX (DCX Human)

Tags

GFP

Expression

Bacterial

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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