We narrowed to 15,480 results for: pET

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pLD1 translation factors

Plasmid

#117760

Purpose

BioBrick encoding cistrons 1-13 in pET for preparation of PURE 3.0 translation system from E. coli

Depositor

Anthony Forster

Article

Shepherd et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

Translation factor cistrons 1-13

Use

Escherichia coli

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLD3 translation factors

Plasmid

#117762

Purpose

BioBrick encoding cistrons 22-30 in pET for preparation of PURE 3.0 translation system from E. coli

Depositor

Anthony Forster

Article

Shepherd et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

Translation factor cistrons 22-30

Use

Escherichia coli

Available Since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pLD2 translation factors

Plasmid

#117761

Purpose

BioBrick encoding cistrons 14-21 in pET for preparation of PURE 3.0 translation system from E. coli

Depositor

Anthony Forster

Article

Shepherd et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

Translation factor cistrons 14-21

Use

Escherichia coli

Available Since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pJH-MBP-CbAgo

Plasmid

#127707

Purpose

pET His6 MBP TEV CbAgo

Depositor

John van der Oost

Article

Hegge et al Nucleic Acids Res. 2019 May 9. pii: 5487

Insert

CbAgo

Tags

MPB

Expression

Bacterial

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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(+)-Limonene Synthase

Plasmid

#112230

Purpose

(+)-Limonene Synthase from Citrus sinensis in pET-28a(+). N-terminal was truncated to remove amino acids 1-52 and then His-tagged for purification.

Depositor

Daniel Oprian

Article

Morehouse et al Biochemistry. 2017 Mar 28;56(12):1706-17

Insert

(+)-Limonene Synthase

Tags

N-terminal His-tag

Expression

Bacterial

Mutation

deleted amino acids 1-52

Available Since

June 29, 2018

Availability

Academic Institutions and Nonprofits only

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pBz15_Strep_Rubisco

Plasmid

#140839

Purpose

wild-type Halothiobacillus neapolitanus Form I Rubisco with StrepII tag (CbbL-StrepII, CbbS), pET-14b backbone

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

Form IA Rubisco; CbbL, CbbS

Tags

Strep II

Expression

Bacterial

Promoter

T7

Available Since

June 22, 2020

Availability

Academic Institutions and Nonprofits only

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S019

Plasmid

#174552

Purpose

pET-iBclxL-v3, His tag for affinity purification

Depositor

Bruno Correia

Article

Shui et al Nat Commun. 2021 Oct 1;12(1):5754. doi:

Insert

iBclxL-v3, Bclxl inhibitor resistant protein

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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S330

Plasmid

#174553

Purpose

pET-iBcl2-v4, His tag for affinity purification

Depositor

Bruno Correia

Article

Shui et al Nat Commun. 2021 Oct 1;12(1):5754. doi:

Insert

ibcl2-v4, bcl2 inhibitor resistant protein

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pAP007

Plasmid

#206809

Purpose

pET-6xHis-MBP-TEV-BabTIR-APAZ/BabAgo - Heterologous BabSPARTA expression

Depositor

Daan Swarts

Article

Finocchio et al Nucleic Acids Res. 2024 Feb 28;52(4):201

Insert

BabSPARTA operon (6xHis-MBP-BabTIR-APAZ and BabAgo)

Tags

6xHis-MBP

Expression

Bacterial

Promoter

T7

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pBz106_CsoS2_MR

Plasmid

#140843

Purpose

His-tagged CsoS2 Middle Region (MR), pET-14b backbone

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CsoS2 Middle Region (MR)

Tags

Hexahistidine tag

Expression

Bacterial

Mutation

only amino acids 257-603

Promoter

T7

Available Since

June 22, 2020

Availability

Academic Institutions and Nonprofits only

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pBz109_CsoS2_NTD

Plasmid

#140842

Purpose

His-tagged CsoS2 N-terminal domain (NTD), pET-14b backbone

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CsoS2 N-terminal domain (NTD)

Tags

Hexahistidine tag

Expression

Bacterial

Mutation

deleted amino acids 262-869

Promoter

T7

Available Since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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pLz47_polyPro_CsoS2_Npeptide

Plasmid

#140845

Purpose

Consensus N*-peptide after polyproline II helix, N-terminal His- tag used as BLI bait, pET-14b backbone

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

polyproline helix with CsoS2 N-peptide

Tags

Hexahistidine tag and Strep-tag II

Expression

Bacterial

Promoter

T7

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBz110_CsoS2_CTD

Plasmid

#140844

Purpose

His-tagged CsoS2 C-terminal domain (CTD), pET-14b backbone

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CsoS2 C-terminal domain (CTD)

Tags

Hexahistidine tag

Expression

Bacterial

Mutation

deleted amino acids 1-594

Promoter

T7

Available Since

May 8, 2020

Availability

Academic Institutions and Nonprofits only

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pCIOX

Plasmid

#51300

Purpose

E.coli expression vector for His8-SUMO tagged recombinant protein

Depositor

Andrea Mattevi

Type

Empty backbone

Tags

His8 + SUMO

Expression

Bacterial

Promoter

T7

Available Since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

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pSG058

Plasmid

#214261

Purpose

PaFtsH1-6xHis cloned between XbaI/NheI in pET-28a(+)

Depositor

Ute Romling

Article

Mawla et al J Biol Chem. 2024 Feb;300(2):105622. doi

Insert

Core genome PaFtsH1 proteinase from Pseudomonas aeruginosa SG17M

Tags

6xHis-tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pBz172_CsoS2_N1_GFP

Plasmid

#140852

Purpose

His-tagged first N-peptide of NTD fused to Superfolder GFP ([N1]-GFP) used for BLI and MST, pET-14b backbone

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CsoS2 NTD repeat 1 fused to GFP

Tags

Hexahistidine tag, Strep-Tag II, and Superfolder …

Expression

Bacterial

Promoter

T7

Available Since

June 19, 2020

Availability

Academic Institutions and Nonprofits only

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pBz175_CsoS2_N4_GFP

Plasmid

#140855

Purpose

His-tagged fourth N-peptide of NTD fused to Superfolder GFP ([N4]-GFP) used for BLI, pET-14b backbone

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CsoS2 NTD repeat 4 fused to GFP

Tags

Hexahistidine tag, Strep-Tag II, and Superfolder …

Expression

Bacterial

Promoter

T7

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBz173_CsoS2_N2_GFP

Plasmid

#140853

Purpose

His-tagged second N-peptide of NTD fused to Superfolder GFP ([N2]-GFP) used for BLI, pET-14b backbone

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CsoS2 NTD repeat 2 fused to GFP

Tags

Hexahistidine tag, Strep-Tag II, and Superfolder …

Expression

Bacterial

Promoter

T7

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBz174_CsoS2_N3_GFP

Plasmid

#140854

Purpose

His-tagged third N-peptide of NTD fused to Superfolder GFP ([N3]-GFP) used for BLI, pET-14b backbone

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CsoS2 NTD repeat 3 fused to GFP

Tags

Hexahistidine tag, Strep-Tag II, and Superfolder …

Expression

Bacterial

Promoter

T7

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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HOXA9 (murine) FLAG pRC/CMV

Plasmid

#8514

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

Insert

HOXA9 (Hoxa9 Mouse)

Tags

FLAG and His

Expression

Mammalian

Mutation

FLAG epitope tag replaces T7 tag in the pET back…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only