We narrowed to 11,122 results for: ARIA

1. pLV-mTurquoise-MLC-IRES-neo

   Plasmid

   #85145

   Purpose

   lentiviral expression of myosin light chain (MLC, MYL9), N-terminally tagged with mTurquoise

   Depositor

   Tobias Meyer

   Article

   Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

   Insert

   mTurquoise-MLC (myosin regulatory light chain, N-terminally tagged with mTurquoise (MYL9 Human)
   

   Use

   Lentiviral

   Tags

   mTurquoise

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-TRE3_ mTFP1- Vamp2

   Plasmid

   #201214

   Purpose

   AAV expression of TRE(TRE3G)-driven, Vamp2-conjugated mTFP1 expression for fluorescent labling of axon terminals

   Depositor

   Tetsuo Yamamori

   Article

   Watakabe et al Neuron. 2023 May 9:S0896-6273(23)00338-0

   Insert

   mTFP1 (from addgene #54613)
   

   Use

   AAV

   Tags

   mouse Vamp2

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   July 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTwist-SARS-CoV-2 Δ18 B.1.351v3

   Plasmid

   #169464

   Purpose

   Encodes SARS-CoV-2 B.1.351v3 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

   Insert

   SARS-CoV-2 B.1.351v3 Spike truncated to remove 18 amino acids (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCyCEN_Lisp2mCherry_hsp70_GFP

   Plasmid

   #137169

   Purpose

   Dual fluorescent reporter for liver stage expression in Plasmodium cynomolgi

   Depositor

   Clemens Kocken

   Article

   Voorberg-van der Wel et al Commun Biol. 2020 Jan 3;3:7. doi: 10.103

   Inserts

   mCherry
   Nanoluc
   dihydrofolate reductase
   Green Fluorescent Protein
   

   Use

   Unspecified

   Tags

   T2A

   Expression

   Mutation

   Promoter

   P. cynomolgi hsp70 and P. cynomolgi lisp2

   Available since

   May 11, 2020

   Availability

   Academic Institutions and Nonprofits only

5. eSpCas9-plus

   Plasmid

   #126767

   Purpose

   Expression plasmid for human codon-optimized increased fidelity eSpCas9-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   eSpCas9-plus
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   K848A, R1060A; amino acids 1005-1013 replaced wit…

   Promoter

   CBh

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPB-cT3G-cERP2-TSC22D3

   Plasmid

   #192916

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of TSC22D3

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   TSC22D3 (TSC22D3 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   TET3G-miniCMV

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCC_02 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9NG-NLS-2A-Puro-WPRE

   Plasmid

   #139087

   Purpose

   Expresses human codon-optimized Cas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

   Depositor

   Neville Sanjana

   Article

   Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

   Insert

   SpCas9-NG
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   L1111R, D1135V,G1218R, E1219F, A1322R, R1335V, T1…

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEB1N-mScarlet-LZ-LOV2

   Plasmid

   #197419

   Purpose

   Mammalian expression of the mScarlet-tagged N-terminal half of photo-inactivated π-EB1

   Depositor

   Torsten Wittmann

   Article

   Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

   Insert

   MAPRE1 (MAPRE1 Human)
   

   Use

   Tags

   A. sativa phototropin 1 LOV2 domain, GCN4 leucine…

   Expression

   Mammalian

   Mutation

   EB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…

   Promoter

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pS6-hα2M-H6

   Plasmid

   #128495

   Purpose

   Expresses human α2M (from G27 to S1468) in mammalian cells. With C-t H6x tag.

   Depositor

   F Xavier Gomis-Ruth

   Article

   Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

   Insert

   A2M (G27-S1468) (A2M Human)
   

   Use

   Tags

   His tag (6x)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-CARMA2sh-HA

    Plasmid

    #97072

    Purpose

    Expresses CARMA2sh/CARD14 isoform 3 in mammalian cells

    Depositor

    Pasquale Vito

    Article

    Scudiero et al J Cell Physiol. 2011 Dec;226(12):3121-31

    Insert

    CARD14 isoform 3 (CARD14 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. LentiU6-hASCL1 gRNA_multi1-3-MS2-Puro

    Plasmid

    #192682

    Purpose

    Lentiviral expression of multi gRNAs targeting hASCL1 promoter to activate human ASCL1 transcription

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Human ASCL1 activating gRNAs #1,2,3 (ASCL1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pT7-G-LbCas12a crRNA-BsaI cassette (MSP3495)

    Plasmid

    #114068

    Purpose

    T7 promoter crRNA entry vector used for in vitro transcription of LbCas12a guides

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    entry vector for LbCas12a crRNAs
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCK668

    Plasmid

    #192637

    Purpose

    Expresses dCas9-NG and MCP-SoxS(R93A/S101A) on p15A-CmR

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

    Inserts

    Sp.pCas9-dCas9-NG
    BBa_J23107-MCP-SoxS(R93A, S101A)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    SoxS has R93A and S101A mutations and dCas9-NG ha…

    Promoter

    BBa_J23107 and Sp.pCas9

    Available since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-CoV2-Omicron BA.1 spike

    Plasmid

    #194602

    Purpose

    Mammalian expression of RBD domain from SARS-CoV-2 omicron (BA.1)

    Depositor

    Ben Murrell

    Article

    Sheward et al Lancet Infect Dis. 2022 Jun;22(6):813-82

    Insert

    CoV2-Omicron BA.1 spike (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPGK-T7/2-CD44v9 (fl)

    Plasmid

    #137818

    Purpose

    expression of CD44 proteins in mammalian cells

    Depositor

    Ursula Gunthert

    Article

    Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

    Insert

    CD44v9 (fl) (CD44 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    full length and mutation H48L

    Promoter

    PGK

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3_SP-GLP1R-V2R-NTEV-TCS-GV-2xHA

    Plasmid

    #194381

    Purpose

    Split TEV assays

    Depositor

    Michael Wehr

    Article

    Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

    Insert

    SP-GLP1R-V2R-NTEV-TCS-GV-2xHA (GLP1R Synthetic, Human)
    

    Use

    Tags

    2xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX-4T-1_RAF1 (51-131)

    Plasmid

    #119218

    Purpose

    purification of GST-RAF1 RBD from E. coli

    Depositor

    Helen Morrison

    Article

    Riecken et al Hum Mutat. 2015 Feb;36(2):270-8. doi: 10

    Insert

    RAF1 aa 51-131 (RAF1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTwist-SARS-CoV-2 Δ18 B.1.351v1

    Plasmid

    #169462

    Purpose

    Encodes SARS-CoV-2 B.1.351v1 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

    Insert

    SARS-CoV-2 B.1.351v1 Spike truncated to remove 18 amino acids (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-CARMA2sh-FLAG

    Plasmid

    #97071

    Purpose

    Expresses CARMA2sh/CARD14 isoform 3 in mammalian cells

    Depositor

    Pasquale Vito

    Article

    Scudiero et al J Cell Physiol. 2011 Dec;226(12):3121-31

    Insert

    CARD14 isoform 3 (CARD14 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV.CAG-FLEX.SF-iGluSnFR.A184V

    Plasmid

    #106187

    Purpose

    Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-iGluSnFR.A184V
    

    Use

    AAV

    Tags

    Expression

    Mutation

    GltI: A184V

    Promoter

    CAG

    Available since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pIE-hα2M-H6

    Plasmid

    #128492

    Purpose

    Expresses human α2M (from S24 to N1473) in insect cells. With C-t H6x tag.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

    Insert

    A2M (A2M Human)
    

    Use

    Tags

    His tag (6x)

    Expression

    Insect

    Mutation

    Promoter

    IE1

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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22. pAAV.GFAP.SF-iGluSnFR.A184V

    Plasmid

    #106193

    Purpose

    Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-iGluSnFR.A184V
    

    Use

    AAV

    Tags

    Expression

    Mutation

    GltI: A184V

    Promoter

    GFAP

    Available since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. LentiU6-hIL2RN gRNA_multi1-3-MS2-Puro

    Plasmid

    #192684

    Purpose

    Lentiviral expression of multi gRNAs targeting hIL1RN promoter to activate human IL1RN transcription

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Human IL1RN activating gRNAs #1,2,3 (IL1RN Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. lenti-Cas9-VQR-Blast

    Plasmid

    #87155

    Purpose

    lentiviral expression of SpCas9-VQR (NGA PAM restricted)

    Depositor

    Daniel Bauer

    Article

    Canver et al Nat Genet. 2017 Feb 20. doi: 10.1038/ng.

    Insert

    SpCas9-VQR(D1135V,R1335Q,T1337R)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    D1135V,R1335Q,T1337R

    Promoter

    Available since

    Feb. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pTE4938

    Plasmid

    #107527

    Purpose

    Expresses Fn crRNA and mCherry in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    Fn crRNA
    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and human U6

    Available since

    April 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. LV_EF1a_CDK1-P2A-Hygro_Barcode

    Plasmid

    #170221

    Purpose

    Barcoded lentiviral vector to express CDK1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    CDK1 (CDK1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pPGK-T7/2-CD44s (-cyt)

    Plasmid

    #137815

    Purpose

    expression of CD44 proteins in mammalian cells

    Depositor

    Ursula Gunthert

    Article

    Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

    Insert

    CD44s (-cyt) (CD44 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    without cytoplasmic region

    Promoter

    PGK

    Available since

    June 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pPGK-T7/2-CD44v6-10 (-cyt)

    Plasmid

    #137821

    Purpose

    expression of CD44 proteins in mammalian cells

    Depositor

    Ursula Gunthert

    Article

    Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

    Insert

    CD44v6-10 (-cyt) (CD44 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    without cytoplasmic region

    Promoter

    PGK

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pTE4495

    Plasmid

    #80338

    Purpose

    mammalian expression MbCpf1 nuclease and Mb crRNA

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    Mb crRNA
    MbCpf1
    

    Use

    CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and human U6

    Available since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV.GFAP.SF-iGluSnFR.S72A

    Plasmid

    #106194

    Purpose

    Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-iGluSnFR.S72A
    

    Use

    AAV

    Tags

    Expression

    Mutation

    GltI: S72A

    Promoter

    GFAP

    Available since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge