We narrowed to 159,371 results for: Gene

1. HA-TRIM28deltaRBCC

   Plasmid

   #124953

   Purpose

   Mammalian expression of Human TRIM28 with deleted RING, Bbox, CC domains

   Depositor

   Michelle Barton

   Article

   Li et al Oncogene. 2017 May 25;36(21):2991-3001.

   Insert

   hTRIM28 (TRIM28 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Deletion of RING, Bbox, and CC domains

   Promoter

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PC17-605-9-1_BRD4-BD2-VPR

   Plasmid

   #200436

   Purpose

   Expresses the VPR transactivation domain linked to the BRD4(BD2) target protein

   Depositor

   Xue Gao

   Article

   Ma et al J Am Chem Soc. 2023 Jan 25;145(3):1593-1

   Insert

   BRD4(BD2)-VPR
   

   Use

   Tags

   BRD4(BD2), VPR

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PC15-421-14-1_TRIM24-BD-VPR

   Plasmid

   #200435

   Purpose

   Expresses the VPR transactivation domain linked to the TRIM24(BD) target protein

   Depositor

   Xue Gao

   Article

   Ma et al J Am Chem Soc. 2023 Jan 25;145(3):1593-1

   Insert

   TRIM24(BD)-VPR
   

   Use

   Tags

   TRIM24(BD), VPR

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. CA-Stat3-t2A-mCherry

   Plasmid

   #127540

   Purpose

   Expresses constitutively active (CA) mouse Stat3 and mCherry via t2A linker.

   Depositor

   Jennifer Mitchell

   Article

   Dhaliwal et al Genes Dev. 2019 Jun 20. pii: gad.324319.

   Insert

   Stat3 (Stat3 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A662C and N664C. Residues changed to Cys renders …

   Promoter

   CMV

   Available since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pPBpuro-ErbB3

   Plasmid

   #197362

   Purpose

   Encoding human ErbB3.

   Depositor

   Michiyuki Matsuda

   Article

   Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

   Insert

   ERBB3 (ERBB3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-hSyn-Cluc-RMA

   Plasmid

   #189624

   Purpose

   Expresses Cluc-RMA under the hSyn promoter. Cluc-RMA for monitoring neuronal transduction.

   Depositor

   Jerzy Szablowski

   Article

   Lee et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Insert

   Cypridina luciferase fused to Fc
   

   Use

   AAV and Luciferase

   Tags

   Cluc and IgG1 Fc

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. iCAP-BI155-KanR

   Plasmid

   #209527

   Purpose

   RepCap for AAV production

   Depositor

   Ben Deverman

   Article

   Eid et al Nat Commun. 2024 Aug 4;15(1):6602. doi:

   Insert

   AAV-BI155 Cap
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LentiCRISPRv2 NT sgRNA3

   Plasmid

   #164929

   Purpose

   Expresses Non-targeting sgRNA3 control in mammalian cells

   Depositor

   Ji Luo

   Article

   Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

   Insert

   Non-targeting sgRNA3 (verified for knockout)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EFS promoter

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-SlugCBE-NNG-P2A-puro

   Plasmid

   #214355

   Purpose

   Expresses SlugCBE-NNG and puromycin resistance genes

   Depositor

   Yongming Wang

   Article

   Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

   Insert

   SlugCBE-NNG and puromycin resistance genes
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Q782R/S888R/L906R/N984S/E1012K/K1016I

   Promoter

   Available since

   March 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pK237.U6-Chimeric-CAG-loxP-stop-loxP-hspCas9 (Supernova)

    Plasmid

    #85041

    Purpose

    This plasmid should be used for Cre/loxP-based Supernova-CRISPR/Cas9.

    Depositor

    Takuji Iwasato

    Article

    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

    Insert

    loxP-stop-loxP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-TRE-DIO-mNeonGreen

    Plasmid

    #99132

    Purpose

    An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein mNeonGreen

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    DIO-mNeonGreen
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBABR IRFP-Bcd-LEXY

    Plasmid

    #182595

    Purpose

    Drosophila integration plasmid expressing IRFP-Bcd-LEXY fusion protein under the maternal tubulin promoter.

    Depositor

    Jared Toettcher

    Article

    Singh et al Cell Rep. 2022 Mar 22;38(12):110543. doi

    Insert

    Bcd (bcd Fly)
    

    Use

    Tags

    IRFP and LEXY

    Expression

    Insect

    Mutation

    Promoter

    maternal tubulin

    Available since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-TRE-DIO-tdTomato

    Plasmid

    #99116

    Purpose

    An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein tdTomato

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    DIO-tdTomato
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. IFNAR2_pCSdest

    Plasmid

    #53914

    Purpose

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    IFNAR2 (IFNAR2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6 and CMV

    Available since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

15. pCG-Hc∆18-AA-IL-13

    Plasmid

    #86171

    Purpose

    Plasmid encodes blinded measles virus H glycoprotein displaying IL-13

    Depositor

    Jakob Reiser

    Article

    Ou et al Hum Gene Ther Methods. 2012 Apr;23(2):13

    Insert

    Measles virus H glycoprotein fused to IL-13
    

    Use

    Tags

    6x His

    Expression

    Mammalian

    Mutation

    Y463A, R515A

    Promoter

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. IFNAR1_pCSdest

    Plasmid

    #53881

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    IFNAR1 (IFNAR1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6 and CMV

    Available since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pLV[Exp]-Neo-EF1A>Tet3G

    Plasmid

    #184379

    Purpose

    The Tet3G gene is expressed under a constitutive EF1-alpha promoter. This protein binds a TRE3G promoter to activate gene transcription only in the presence of tetracycline or its analogs (e.g. doxycycline)

    Depositor

    Ori Bar-Nur

    Article

    Domenig et al Stem Cell Reports. 2022 Feb 8;17(2):321-

    Insert

    Tet3G
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Human NCKX3-FlagTag (pcDNA3.1-)

    Plasmid

    #75206

    Purpose

    Mammalian expression of SLC24A3

    Depositor

    Jonathan Lytton

    Article

    Kraev et al J Biol Chem. 2001 Jun 22;276(25):23161-7

    Insert

    SLC24A3 (SLC24A3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 3xFLAG-CUL4A-pCMV7.1

    Plasmid

    #155022

    Purpose

    N-terminal 3xFLAG-tagged CUL4A in a pCMV7.1 vector

    Depositor

    Andre Catic

    Article

    Sweeney et al Sci Rep. 2020 Aug 18;10(1):13942. doi: 1

    Insert

    Cullin 4A (CUL4A Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    Promoter

    pCMV7.1

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV2-SynTetOff-palGFP

    Plasmid

    #190232

    Purpose

    AAV vector, high-level expression of EGFP with a palmitoylation signal derived from GAP-43 N-terminus (palGFP) in neuronal cells

    Depositor

    Hiroyuki Hioki

    Article

    Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

    Insert

    EGFP
    

    Use

    AAV

    Tags

    palmitoylation signal sequence

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV-GFAP-mKate2.5f

    Plasmid

    #99129

    Purpose

    Also known as pAAV-GFAP-mKate2.5-f. An AAV genome that express the farnesylated fluorescent protein mKate2.5 from the GFAP promoter

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    mKate2.5f
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    GFAP

    Available since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA5/FRT-mMAPT

    Plasmid

    #231584

    Purpose

    Minigene of mouse MAPT containing exon 9, 10, 11 and relevant intronic sequences

    Depositor

    Chaolin Zhang

    Article

    Recinos et al Cell Genom. 2024 Jun 12;4(6):100563. doi

    Insert

    mouse MAPT (Mapt Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV2-SynTetOff-myrGFP

    Plasmid

    #190233

    Purpose

    AAV vector, high-level expression of EGFP with a myristoylation/palmitoylation signal derived from Fyn N-terminus (myrGFP) in neuronal cells

    Depositor

    Hiroyuki Hioki

    Article

    Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

    Insert

    EGFP
    

    Use

    AAV

    Tags

    myristoylation signal sequence

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-CaMKII-GAL4-iLight-VP16

    Plasmid

    #170274

    Purpose

    NLS-Gal4(1-65)-iLight(human codon optimized)-VP16 construct expression in mammalian cells. pAAV vector.

    Depositor

    Vladislav Verkhusha

    Article

    Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

    Insert

    NLS-Gal4(1-65)-iLight(human codon optimized)-VP16
    

    Use

    AAV

    Tags

    Expression

    Mutation

    See Comments

    Promoter

    CaMKII

    Available since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pYDE009

    Plasmid

    #194151

    Purpose

    Delivery of dcas9 into A. baumannii via Tn7 for CRISPRi. Tn7 element has tetR-tetP-dcas9, GmR; plasmid backbone is CarbR.

    Depositor

    Edward Geisinger

    Article

    Bai et al J Bacteriol. 2021 May 20;203(12):e005652

    Insert

    tetR-tetP-dcas9-rrnB T1-T7Te
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tetp

    Available since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. (364) SB KRAB-SadCas9

    Plasmid

    #163022

    Purpose

    Sleeping Beauty TET-On expression of CRISPRi (without U6-gRNA)

    Depositor

    Tonia Rex

    Article

    Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

    Insert

    Mammalian codon-optimized SadCas9
    

    Use

    Transposon

    Tags

    KRAB and myc NLS

    Expression

    Mammalian

    Mutation

    Promoter

    TET-On

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAC93-pmax-dCas9VP160

    Plasmid

    #48225

    Purpose

    dCas9VP160 on pmax expression vector

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9(D10A;H840A) fusion with VP160 activation domain
    

    Use

    CRISPR

    Tags

    HA Tag and VP160

    Expression

    Mammalian

    Mutation

    D10A;H840A (catalytically inactive)

    Promoter

    CAGGS

    Available since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMGS56 (GFP-ARF16-PB1-P2A-OsTIR1)

    Plasmid

    #129668

    Purpose

    A repair construct to express GFP-ARF16-PB1 and OsTIR1 under the control of the CMV promoter from the human AAVS1 locus

    Depositor

    Michael Guertin

    Article

    Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

    Insert

    OsARF16-PB1 domain
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCAS1yl

    Plasmid

    #73226

    Purpose

    Constitutive expression of Cas9 and sgRNA in Yarrowia lipolytica cells

    Depositor

    Sheng Yang

    Article

    Gao et al J Ind Microbiol Biotechnol. 2016 Aug;43(

    Inserts

    Cas9
    sgRNA
    

    Use

    CRISPR; In y. lipolytica

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    unknown

    Available since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. CYFIP1.mCherry

    Plasmid

    #122051

    Purpose

    Expresses human CYFIP1 in mammalian cells.

    Depositor

    Josef Kittler

    Article

    Pathania et al Transl Psychiatry. 2014 Mar 25;4:e374. d

    Insert

    CYFIP1 (CYFIP1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLV_hUbC-dSpCas9-2xVP64-T2A-BSD

    Plasmid

    #162333

    Purpose

    Lentiviral expression of dSpCas9-2xVP64 with blasticidin resistance

    Depositor

    Charles Gersbach

    Article

    Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

    Insert

    dSpCas9-2xVP64
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D10A and H840A

    Promoter

    hUbC

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. proE-cad670-Luc

    Plasmid

    #42083

    Depositor

    Kumiko Ui-Tei

    Article

    Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

    Insert

    E-cadherin (CDH1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    contains E-cadherin promoter region from -670 to …

    Promoter

    E-cadherin

    Available since

    March 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pKRG3-mU6-PUb-hSpCas9

    Plasmid

    #162162

    Purpose

    Expresses hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)

    Depositor

    Charles Rice

    Article

    Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

    Inserts

    hSpCas9
    sgRNA
    

    Use

    CRISPR

    Tags

    N/A

    Expression

    Insect

    Mutation

    Promoter

    Ae. aegypti PUb and Ae. aegypti U6

    Available since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pSLS.419

    Plasmid

    #187208

    Purpose

    Expresses wild-type retron Eco4 ncRNA, and Cas1 + Cas2

    Depositor

    Seth Shipman

    Article

    Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

    Inserts

    Retron Eco4 ncRNA
    Cas1 + Cas2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7/Lac

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pOME0182

    Plasmid

    #178403

    Purpose

    Expresses KSHV K8.1 glycoprotein hyp tagged

    Depositor

    Dirk Dittmer

    Article

    Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

    Insert

    KSHV K8.1
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pTet-O-OVOL1-T2A-PuroR

    Plasmid

    #162347

    Purpose

    Lentiviral expression of OVOL1 under the control of the TetON promoter

    Depositor

    Charles Gersbach

    Article

    Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

    Insert

    OVOL1 (OVOL1 Human)
    

    Use

    Lentiviral

    Tags

    T2A-PuroR

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-ON

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pSL1180-HR-Aag2-loxP-PUb-RFP-loxP-3xFLAG-AGO1

    Plasmid

    #162164

    Purpose

    Selectable homology donor template for Aag2 cell AGO1

    Depositor

    Charles Rice

    Article

    Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

    Inserts

    RFP
    3xFLAG
    

    Use

    CRISPR and Cre/Lox; Homology donor template

    Tags

    AGO1 homology and loxP

    Expression

    Insect

    Mutation

    Promoter

    Ae. aegypti PUb and endogenous AGO1

    Available since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. CAG-DDdCas9VP192-T2A-EGFP-ires-puro

    Plasmid

    #69534

    Purpose

    DHFR destabilised domain (DD) fused to dCas9VP192 (S.pyogenes) on CAG expression vector. DDdCas9VP192 protein is stabilised by Trimethoprim.

    Depositor

    Timo Otonkoski

    Article

    Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

    Insert

    DD-dCas9VP192-T2A-EGFP
    

    Use

    Tags

    C-term T2A-EGFP and DHFR Destabilised Domain

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. th-P2A-EGFP donor

    Plasmid

    #65562

    Purpose

    Intron targeting-mediated EGFP knockin at the zebrafish th locus.

    Depositor

    Jiu-lin Du

    Article

    Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20

    Insert

    th-P2A-EGFP (th Zebrafish)
    

    Use

    Zebrafish injection

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    June 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pTRE-Tight-BI-Gl NORM-LacZA-TER-LacZB

    Plasmid

    #86194

    Purpose

    beta Globin NMD construct with and without PTC under Bi-directional TET on promoter

    Depositor

    Robert Singer

    Article

    Trcek et al Genes Dev. 2013 Mar 1;27(5):541-51. doi:

    Insert

    beta Globin (HBB, Hbb Mouse, Human)
    

    Use

    Tet on

    Tags

    Expression

    Mammalian

    Mutation

    39TER in beta Globin in MCS II

    Promoter

    Tet on

    Available since

    Jan. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pAH-CTX1-rhadCas9

    Plasmid

    #129391

    Purpose

    Derived from pAH-CTX1-rha. The Burkholderia cenocepacia codon-optimized dCas9 cloned downstream of PrhaBAD. Hence, dCas9 is controlled by the rhamnose-inducible promoter system.

    Depositor

    Silvia Cardona

    Article

    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

    Insert

    Burkholderia cenocepacia codon-optimized dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Entire dCas9 codon optimized for the GC-rich B. c…

    Promoter

    PrhaBAD

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pSW610 - GR-LhG4_BD

    Plasmid

    #115992

    Purpose

    entry vector for GreenGate cloning method, CDS, with adaptors for B and D modules

    Depositor

    Sebastien Wolf

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    GR-LhG4_BD
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pB[nos-Cas9 PUb-ZsGreen]

    Plasmid

    #169011

    Purpose

    For germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.

    Depositor

    Max Scott

    Article

    Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

    Insert

    Cas9
    

    Use

    Piggybac

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Drosophila melanogaster nanos

    Available since

    April 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pSH1/S-Fvls-p30Casp9-E

    Plasmid

    #15272

    Depositor

    David Spencer

    Article

    Fan et al Hum Gene Ther. 1999 Sep 20. 10(14):2273-

    Insert

    FKBP12(V36)-p30Caspase9 (CASP9 Human)
    

    Use

    Tags

    HA epitope and Myristoylation-targeting domain c-…

    Expression

    Mammalian

    Mutation

    FKBP12 F36 to VTruncated Caspase9 (135-416; lacki…

    Promoter

    Available since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. LentiCRISPRv2 EGFP sgRNA2

    Plasmid

    #164933

    Purpose

    Expresses EGFP sgRNA2 in mammalian cells

    Depositor

    Ji Luo

    Article

    Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

    Insert

    sgRNA2 targeting Enhanced green fluorescent protein (verified for knockout)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS promoter

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. PB-CAG-CasRX-P2A-EGFP-BGH PolyA

    Plasmid

    #154004

    Purpose

    Express CasRx

    Depositor

    Hui Yang

    Article

    He et al Protein Cell. 2020 Mar 18. pii: 10.1007/

    Insert

    CasRx
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. MSCVhygro-F-Ezh2-F667I

    Plasmid

    #24927

    Depositor

    Kai Ge

    Article

    Wang et al Proc Natl Acad Sci U S A. 2010 Apr 20. 1

    Insert

    Ezh2 (EZH2 Mouse)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Phenylanaline(F)667 mutated to Isoleucine(I)

    Promoter

    Available since

    July 15, 2010

    Availability

    Academic Institutions and Nonprofits only

48. pSH231-EF1-Puro

    Plasmid

    #115143

    Purpose

    Safe harbor site 231 knock-in vector with puromycin resistance selection cassette

    Depositor

    Raymond Monnat, Michael Phelps

    Article

    Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

    Insert

    Puromycin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pAAV-TRE-DIO-mTurquoise2

    Plasmid

    #99115

    Purpose

    An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein mTurquoise2

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    DIO-mTurquoise2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pM2AH + mSlo1

    Plasmid

    #16152

    Depositor

    Larry Salkoff

    Article

    Butler et al Science. 1993 Jul 9. 261(5118):221-4.

    Insert

    Mus musculus potassium large conductance calcium-activated channel (Kcnma1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available since

    Jan. 10, 2008

    Availability

    Academic Institutions and Nonprofits only