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We narrowed to 8,750 results for: Dos

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Showing: 4601 - 4620 of 8750 results

  1. pDG459

    Plasmid
    #100901
    Purpose
    SpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG and T2A-PuroR
    Expression
    Mammalian
    Promoter
    CBh and U6
    Available Since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100901-plasmid-map-sequence-id-197197

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  2. pCMV-IRES-Renilla Luciferase-IRES-Gateway-Firefly Luciferase (pIRIGF)

    Plasmid
    #101139
    Purpose
    Luciferase reporter plasmid. Note that both renilla luciferase and ORF-firefly luciferase are encoded by a single mRNA and both are translated independently via internal ribosomal binding sites.
    Depositor
    William Kaelin
    Article
    Lu et al Science. 2014 Jan 17;343(6168):305-9. do
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    101139-plasmid-map-sequence-id-194900

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  8. FZ-NbPDS

    Plasmid
    #184268
    Purpose
    pAIDE containing a full-length CPSMV RNA2 cDNA modified to accomodate an NbPDS fragment.
    Depositor
    Feng Qu
    Article
    Zaulda et al Plant Methods. 2022 Oct 28;18(1):116. do
    Insert
    CPSMV RNA2 cDNA, modified to permit foreign gene insertion between MP and L-CP
    Expression
    Plant
    Mutation
    The protease processing site between MP and L-CP …
    Promoter
    CaMV 35S with duplicated enhancer
    Available Since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    184268-plasmid-map-sequence-id-363057

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  10. pICE-FLAG-NAT10-siR-WT

    Plasmid
    #59365
    Purpose
    Plasmid for constitutive or doxycycline-inducible expression of wild-type human NAT10 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.
    Depositor
    Steve Jackson
    Article
    Larrieu et al Science. 2014 May 2;344(6183):527-32. do
    Insert
    human NAT10 (NAT10 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Mutations to render cDNA resistant to a siRNA
    Promoter
    CMV-tet
    Available Since
    Oct. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    59365-plasmid-map-sequence-id-92043

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  12. pICE-FLAG-NAT10-siR-G641E

    Plasmid
    #59366
    Purpose
    Plasmid for constitutive or doxycycline-inducible expression of G641E mutant of human NAT10 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.
    Depositor
    Steve Jackson
    Article
    Larrieu et al Science. 2014 May 2;344(6183):527-32. do
    Insert
    NAT10 NM_024662 (NAT10 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    G641E and silent mutations to render the cDNA res…
    Promoter
    CMV-tet
    Available Since
    Oct. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    59366-plasmid-map-sequence-id-92067

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Showing: 4601 - 4620 of 8750 results