We narrowed to 5,112 results for: codon optimized

1. actc1b-2PH-PLCd-mKate2

   Plasmid

   #109500

   Purpose

   Muscle specific zebrafish expression of mKate2 fused to 2x the PH domain from Human phospholipase C delta 1 (PLCD1, codon optimised). Binds PtdIns(4,5)P2.

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   actc1b-2PH-PLCd-mKate2
   

   Use

   Zebrafish plasmids

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTU1-B-SP44s-GUS

   Plasmid

   #172430

   Purpose

   Expresses Streptomyces codon-optimised beta-D-glucuronidase

   Depositor

   Simon Moore, Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2021 Feb 19;10(2):402-41

   Insert

   beta-D-glucuronidase
   

   Use

   Synthetic Biology

   Tags

   None

   Expression

   Bacterial

   Promoter

   SP44s

   Available Since

   Aug. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1_FRB-GEM23-Halo

   Plasmid

   #197060

   Purpose

   Expression of nanocage I3-01 Halo-FRB-fusion in mammalian cells

   Depositor

   Julia Mahamid

   Article

   Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

   Insert

   I3-01
   

   Tags

   FRB and HaloTag

   Expression

   Mammalian

   Mutation

   Codon-optimised for human cell expression

   Available Since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. actc1b-mKate2-ING2

   Plasmid

   #109502

   Purpose

   Muscle specific zebrafish expression of mKate2 fused to 3xPHD domain from Mouse inhibitor of growth family member 2 (ING2), codon optimised. Binds PtdIns5P

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   actc1b-mKate2-ING2
   

   Use

   Zebrafish plasmids

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSI HA Nup53xl

   Plasmid

   #86000

   Purpose

   mammalian expression of HA tagged Xenopus laevis Nup53

   Depositor

   Wolfram Antonin

   Article

   Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

   Insert

   Xenopus laevis Nup53 (nup35.L Frog)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   codon optimzed for bacterial expression

   Available Since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSI myc Nup53xl

   Plasmid

   #86001

   Purpose

   mammalian expression of myc tagged Xenopus laevis Nup53

   Depositor

   Wolfram Antonin

   Article

   Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

   Insert

   Xenopus laevis Nup53 (nup35.L Frog)
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   codon optimzed for bacterial expression

   Available Since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-UniSAM

   Plasmid

   #99866

   Purpose

   Encodes for Cas9-VP64, MS2-p65-HSF1, mCherry and for the gRNA 2.0

   Depositor

   Lesley Forrester

   Article

   Fidanza et al Sci Rep. 2017 Jul 25;7(1):6394. doi: 10.

   Insert

   UniSAM-mCherry + U6-gRNA2.0
   

   Use

   CRISPR and Synthetic Biology; Dcas9-sam activation

   Expression

   Mammalian

   Mutation

   BbsI sites in CDS were ablated by consensus mutat…

   Promoter

   EF1a

   Available Since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLIB-HIS-TEV-Cyclin-B

   Plasmid

   #177012

   Purpose

   Generate baculovirus for insect cell expression of Cyclin-B with N-terminal Polyhistidine-TEV

   Depositor

   Andrea Musacchio

   Article

   Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

   Insert

   Cyclin B1 (CCNB1 Human)
   

   Tags

   Polyhistidine-TEV

   Expression

   Insect

   Mutation

   codon-optimised for insect cell expression

   Available Since

   Nov. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. MSP6-bio

   Plasmid

   #47732

   Purpose

   Expresses enzymatically monobiotinylated full-length MSP6 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

   Insert

   Codon-optimised MSP6
   

   Tags

   enzymatic biotinylation sequence and rat Cd4 d3+4

   Expression

   Mammalian

   Mutation

   Exogenous signal peptide of mouse origin, changed…

   Promoter

   CMV

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MSP11-bio

    Plasmid

    #47734

    Purpose

    Expresses enzymatically monobiotinylated full-length MSP11 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised MSP11
    

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. AARP-bio

    Plasmid

    #47783

    Purpose

    Expresses enzymatically monobiotinylated full-length AARP ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised AARP
    

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MSP4-bio

    Plasmid

    #47717

    Purpose

    Expresses enzymatically monobiotinylated full-length MSP4 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised MSP4
    

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LLP777_SnoopCatcher-p65HSF1-CO

    Plasmid

    #211770

    Purpose

    SnoopTag counterpart binding domain, SnoopCatcher, fused to transcriptional activator p65HSF1, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    SnoopCatcher-p65HSF1
    

    Tags

    2xOLLAS

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect p65HSF…

    Promoter

    pEF1a and pSV40

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LLP775_αGCN4-p65HSF1-CO

    Plasmid

    #211768

    Purpose

    SunTag counterpart binding domain, aGCN4, fused to transcriptional activator p65HSF1, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    aGCN4-p65HSF1
    

    Tags

    3xTy1

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect p65HSF…

    Promoter

    pEF1a and pSV40

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRSETa-mMaple3-V157I

    Plasmid

    #98577

    Purpose

    photoconvertible fluorescent protein mMaple3-V157I in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression, sequence is codon optimised for E. coli

    Depositor

    Ulrike Endesfelder

    Article

    Turkowyd et al Angew Chem Int Ed Engl. 2017 Jun 2. doi:

    Insert

    mMaple3-V157I
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. LLP778_Traptavidin-p65HSF1-CO

    Plasmid

    #211771

    Purpose

    AviTag counterpart binding domain, Traptavidin, fused to transcriptional activator p65HSF1, with sfGFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    Traptavidin-p65HSF1
    

    Tags

    3xV5

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect p65HSF…

    Promoter

    pEF1a and pSV40

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. LLP776_SpyCatcher-p65HSF1-CO

    Plasmid

    #211769

    Purpose

    SpyTag counterpart binding domain, SpyCatcher, fused to transcriptional activator p65HSF1, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    SpyCatcher-p65HSF1
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect p65HSF…

    Promoter

    pEF1a and pSV40

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. RH5-bio

    Plasmid

    #47780

    Purpose

    Expresses enzymatically monobiotinylated full-length RH5 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised RH5
    

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LLP617_αGCN4-mCherry

    Plasmid

    #211785

    Purpose

    SunTag counterpart binding domain, aGCN4, fused to catalytically inert mCherry control, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    SnoopCatcher-DNMT3A
    

    Tags

    2xOLLAS

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect DNMT3A…

    Promoter

    pEF1a and pSV40

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LLP783_SpyCatcher-DNMT3A-CO

    Plasmid

    #211776

    Purpose

    SpyTag counterpart binding domain, SpyCatcher, fused to DNA methyltransferase DNMT3A, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    Traptavudin-DNMT3A
    

    Tags

    3xV5

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect DNMT3A…

    Promoter

    pEF1a and pSV40

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge