We narrowed to 5,457 results for: golden gate

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pWN080

Plasmid

#233820

Purpose

MoClo-YTK; type3B; NS3 systems; Linker-NS3a

Depositor

Jeff Hasty

Publication

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

Linker-NS3a

Expression

Yeast

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pWN043

Plasmid

#233821

Purpose

MoClo-YTK; type3B; NS3 systems; DNCR2

Depositor

Jeff Hasty

Publication

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

DNCR2

Expression

Yeast

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pWN062

Plasmid

#233822

Purpose

MoClo-YTK; type3A; NS3 systems; LexA-NES

Depositor

Jeff Hasty

Publication

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

LexA-NES

Expression

Yeast

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO176

Plasmid

#233824

Purpose

MoClo-YTK; type3A; Activation domains; NLS-VP48

Depositor

Jeff Hasty

Publication

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

NLS-VP48

Expression

Yeast

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-mRuby2(miR.FF5)-TS.FF6x1-bGH

Plasmid

#235276

Purpose

ComMAND open-loop circuit regulating mRuby2 with miR-FF5

Depositor

Kate Galloway

Publication

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-TS.FF4x1-mRuby2(miR.FF4)-bGH

Plasmid

#235285

Purpose

ComMAND closed-loop circuit regulating mRuby2 with miR-FF4 design 2

Depositor

Kate Galloway

Publication

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-EGFP-FMRP-bGH

Plasmid

#235265

Purpose

ComMAND EGFP-FMRP base gene

Depositor

Kate Galloway

Publication

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

EGFP-Fmr1 (Fmr1 Mouse)

Use

Synthetic Biology

Tags

FLAG

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-mRuby2(miRE.FF4)-TS.FF6x1-bGH

Plasmid

#235270

Purpose

ComMAND open-loop circuit regulating mRuby2, EF1a

Depositor

Kate Galloway

Publication

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-mRuby2(miRE.FF4)-TS.FF4x1-bGH

Plasmid

#235271

Purpose

ComMAND closed-loop circuit regulating mRuby2, EF1a

Depositor

Kate Galloway

Publication

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-TS.FF4x1-mRuby2(miRE.FF4)-bGH

Plasmid

#235289

Purpose

ComMAND closed-loop circuit regulating mRuby2 with miRE-FF4 design 2

Depositor

Kate Galloway

Publication

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-mRuby2(miR.FF4)-TS.FF4x1-bGH

Plasmid

#235283

Purpose

ComMAND closed-loop circuit regulating mRuby2 with miR-FF4

Depositor

Kate Galloway

Publication

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-TS.FF6x1-mRuby2(miRE.FF5)-bGH

Plasmid

#235262

Purpose

ComMAND open-loop circuit regulating mRuby2 with miRE-FF5 design 2

Depositor

Kate Galloway

Publication

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-EGFP(miRE.FF4)-FMRP-TS.FF6x1-bGH

Plasmid

#235266

Purpose

ComMAND EGFP-FMRP open-loop circuit

Depositor

Kate Galloway

Publication

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

EGFP-Fmr1 (Fmr1 Mouse)

Use

Synthetic Biology

Tags

FLAG

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-TS.FF4x1-mRuby2-bGH

Plasmid

#235274

Purpose

3' microRNA target site expression for dual-vector iFFL circuit (closed-loop)

Depositor

Kate Galloway

Publication

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-TS.FF6x1-mRuby2(miRE.FF4)-bGH

Plasmid

#235288

Purpose

ComMAND open-loop circuit regulating mRuby2 with miRE-FF4 design 2

Depositor

Kate Galloway

Publication

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-TS.FF6x1-mRuby2-miRE.FF4-bGH

Plasmid

#235290

Purpose

ComMAND open-loop circuit regulating mRuby2 with miRE-FF4 design 3

Depositor

Kate Galloway

Publication

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiX1-[TRE3G-EGFP(miRE.FF4)-FMRP-TS.FF6x1-bGH]-EFS-rtTA-P2A-mRuby2-WPRE

Plasmid

#235307

Purpose

Lentiviral expression of ComMAND open-loop circuit regulating EGFP-FMRP (therapeutically relevant gene)

Depositor

Kate Galloway

Publication

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

EGFP-Fmr1 (Fmr1 Mouse)

Use

Lentiviral and Synthetic Biology

Tags

FLAG

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiX1-[TRE3G-TS.FF6x1-mRuby2-miRE.FF4-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

Plasmid

#235323

Purpose

Lentiviral expression of ComMAND open-loop circuit regulating mRuby2, design 3

Depositor

Kate Galloway

Publication

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-EGFP(miRE.FF4)-FMRP-TS.FF4x1-bGH

Plasmid

#235267

Purpose

ComMAND EGFP-FMRP closed-loop circuit

Depositor

Kate Galloway

Publication

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

EGFP-Fmr1 (Fmr1 Mouse)

Use

Synthetic Biology

Tags

FLAG

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EFS-mRuby2(miRE.FF4)-TS.FF4x1-bGH

Plasmid

#235310

Purpose

ComMAND closed-loop circuit regulating mRuby2, EFS-driven

Depositor

Kate Galloway

Publication

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EFS (human EF1a short, intronless)

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge