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pMaCTag-P08

Plasmid

#120019

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mClover3

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mClover3

Use

Pcr template

Tags

mClover3

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-34

Plasmid

#119998

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with 3xFLAG

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

3xFLAG

Use

Pcr template

Tags

3xFLAG

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-15

Plasmid

#119994

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with miRFP670

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

miRFP670

Use

Pcr template

Tags

miRFP670

Promoter

None

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-GGG-ELP(40nm)-Cys

Plasmid

#91571

Purpose

Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]2, with N-Terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine

Depositor

Hermann Gaub

Publication

Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

Insert

GGG-ELP(40nm)-Cys

Tags

Cysteine and Sortase-Tag

Expression

Bacterial

Promoter

T7

Available since

Feb. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-Cys-ELP(40nm)-LPETGG

Plasmid

#90475

Purpose

Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]2, with N-Terminal Cysteine and C-Terminal Sortase Recognition Sequence LPETGG

Depositor

Hermann Gaub

Publication

Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

Insert

Cys-ELP(40nm)-LPETGG

Tags

Cysteine and Sortase-Tag

Expression

Bacterial

Promoter

T7

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pBSU6-shTim23/CMV-eGFP

Plasmid

#89795

Purpose

Expresses shRNA against TIM23 with a separate promoter for expression of GFP.

Depositor

Robert Friedlander

Publication

Yano et al Nat Neurosci. 2014 Jun;17(6):822-31. doi

Insert

shRNA Tim23 (Timm23 Mouse)

Tags

eGFP under separate promoter

Expression

Mammalian

Promoter

U6

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pFastBac1-OSF-TRIM5alpha(human)

Plasmid

#79034

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Publication

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5alpha(human) (TRIM5 Human)

Tags

OneSTrEP-FLAG

Expression

Insect

Promoter

polyhedrin

Available since

March 3, 2017

Availability

Academic Institutions and Nonprofits only

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CSII-IDR2-owlTRIMCyp-FOS

Plasmid

#79067

Purpose

Express owl monkey TRIMCyp in mammalan cells

Depositor

Wesley Sundquist

Publication

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

TRIM5Cyp (owl monkey)-FOS

Tags

OneSTrEP-FLAG

Expression

Mammalian

Promoter

CMV

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Kohinoor-mitochondria/pcDNA3

Plasmid

#67778

Purpose

Mitochondria-targetted Kohinoor for mammalian cell expression

Depositor

Takeharu Nagai

Publication

Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

Insert

Kohinoor

Tags

CoxVIII x 2

Expression

Mammalian

Promoter

CMV

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only
Pr-deGFP

Plasmid

#67743

Purpose

Pr promoter expressing deGFP

Depositor

Richard Murray

Publication

Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

Insert

UTR1-deGFP-T500

Use

Synthetic Biology

Expression

Bacterial

Promoter

OR2-OR1-Pr

Available since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

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mOrange pHis-//

Plasmid

#53304

Purpose

Empty vector to express N-terminal His-mOrange-tagged fusion protein.

Depositor

John Lis

Publication

Tome et al Nat Methods. 2014 Jun;11(6):683-8. doi:

Type

Empty backbone

Tags

His and mOrange

Expression

Bacterial

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pCDF1-ADAM29 (wt)

Plasmid

#31143

Depositor

Yardena Samuels

Publication

Wei et al Hum Mutat. 2011 Jun;32(6):E2148-75. doi:

Insert

ADAM29 (ADAM29 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

WT

Available since

July 22, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ADAM7 (wt)

Plasmid

#31139

Depositor

Yardena Samuels

Publication

Wei et al Hum Mutat. 2011 Jun;32(6):E2148-75. doi:

Insert

ADAM7 (ADAM7 Human)

Tags

FLAG

Expression

Mammalian

Mutation

wt

Available since

July 22, 2011

Availability

Academic Institutions and Nonprofits only
pDASH-AIK

Plasmid

#242525

Purpose

Acceptor vector I, accepting Donor I first, with attPTT-FRT

Depositor

Jose Alonso, Anna Stepanova

Publication

Zhao et al Plant Biotechnol J. 2025 Jun 10. doi: 10

Type

Empty backbone

Use

Synthetic Biology

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pBI-BFP-NL1

Plasmid

#206153

Purpose

pBI-BFP-NL1

Depositor

Erika Holzbaur

Publication

Dou et al J Cell Biol. 2024 Jun 3;223(6):e20230709

Insert

Neuroligin-1 (Nlgn1 Mouse)

Tags

Bicistronic BFP

Expression

Mammalian

Promoter

CMV

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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CMV HA-MADD

Plasmid

#206150

Purpose

CMV HA-MADD

Depositor

Erika Holzbaur

Publication

Dou et al J Cell Biol. 2024 Jun 3;223(6):e20230709

Insert

MADD (MADD Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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CMV SNAP-Synapsin

Plasmid

#206152

Purpose

CMV SNAP-Synapsin

Depositor

Erika Holzbaur

Publication

Dou et al J Cell Biol. 2024 Jun 3;223(6):e20230709

Insert

Synapsin

Tags

SNAPf

Expression

Mammalian

Promoter

CMV

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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ISA431: aTc-MP6

Plasmid

#189935

Purpose

Mutagenesis Plasmid: pTet, danQ926, dam, seqA, emrR, ugi and cda1, tetR, CloDF13, KanR

Depositor

Nathan Crook

Publication

Al'Abri et al Nucleic Acids Res. 2022 Jun 10;50(10):e5

Insert

danQ926, dam, seqA, emrR, ugi and cda1

Expression

Bacterial

Promoter

pTet

Available since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pIT5_KP_LacI

Plasmid

#173780

Purpose

Bacterial genomic integration vector, LacI repressor

Depositor

Keith Shearwin

Publication

Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pIT5_KH_VanR

Plasmid

#173779

Purpose

Bacterial genomic integration vector, VanR repressor

Depositor

Keith Shearwin

Publication

Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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