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pEDF-PhdRS

Plasmid

#127445

Purpose

Double mutant of wild type mmPylRS designed for incorporation of non-cannonical amino acids in to M13 bacteriophage

Depositor

Wenshe Liu

Publication

Tharp et al Nat Commun. 2020 Mar 13;11(1):1392. doi:

Insert

Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair

Expression

Bacterial

Mutation

N346A-C348A mutations on PylRS

Promoter

araBAD

Available since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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TVBB C-term-Dendra2

Plasmid

#169216

Purpose

Targeting vector backbone to support a knock-in of Linker-Dendra2 at the C-terminus of a target locus

Depositor

Hugo Snippert

Publication

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked Dendra2-P2A-Puro

Use

Targeting vector backbone

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pHH0103_NR0B1_Hormone-recep

Plasmid

#109835

Purpose

Protein expression and purification of NR0B1_Hormone-recep

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

NR0B1_Hormone-recep (NR0B1 Human)

Expression

Bacterial

Available since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_HLA-DRB1_MHC-II-beta

Plasmid

#110021

Purpose

Protein expression and purification of HLA-DRB1_MHC-II-beta

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

HLA-DRB1_MHC-II-beta (HLA-DRB1 Human)

Expression

Bacterial

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_NR5A1_Hormone-recep

Plasmid

#109792

Purpose

Protein expression and purification of NR5A1_Hormone-recep

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

NR5A1_Hormone-recep (NR5A1 Human)

Expression

Bacterial

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pEF-pIgH-ESCargo(FTV,Orange)

Plasmid

#140161

Purpose

Expresses a regulatable secretory cargo for mammalian cells

Depositor

Benjamin Glick

Publication

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

piGH-FTVNTT-E2-Orange-FKBP(LV)(C22V)

Tags

ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

Expression

Mammalian

Promoter

EF-1a

Available since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-V5-Mfsd10

Plasmid

#120238

Purpose

Expresses V5-tagged Mfsd10 in lentiviral vector

Depositor

Larry Gerace

Publication

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Insert

Mfsd10 (Mfsd10 Mouse)

Use

Lentiviral

Tags

V5

Promoter

EF1a

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-HF1-PGK-Puro

Plasmid

#110857

Purpose

Lentiviral vector for constitutive expression of Cas9-HF1 (not codon optimized)

Depositor

Lukas Dow

Publication

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-HF1

Use

Lentiviral

Tags

3X FLAG

Mutation

N497A, R661A, Q695A, Q926A

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pCMB-TMEg

Plasmid

#61209

Purpose

Fluorescent reporter for plasma membrane and trunk peri-arbuscular membrane MtBCPsp-GFP-BCP expressed under MtBCP1 promoter

Depositor

Maria Harrison

Publication

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtBCPsp-GFP-BCP

Tags

EGFP

Expression

Plant

Promoter

MtBCP1

Available since

April 10, 2015

Availability

Academic Institutions and Nonprofits only

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miniSOG-Zyxin-6

Plasmid

#57781

Purpose

Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Publication

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Zyxin (ZYX Human)

Tags

miniSOG

Expression

Mammalian

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles
miniSOG-Keratin-17

Plasmid

#57770

Purpose

Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Publication

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Keratin (KRT18 Human)

Tags

miniSOG

Expression

Mammalian

Mutation

V282L

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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PA-mCherry-miniSOG-Talin-N-10

Plasmid

#57797

Purpose

Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Publication

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Talin

Tags

PA-mCherry-miniSOG

Expression

Mammalian

Promoter

CMV

Available since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
GPKAnes

Plasmid

#61092

Purpose

GFP-tagged PKA catalytic subunit targeted to the extranuclear cytoplasm by addition of a nuclear export signal

Depositor

Steven Green

Publication

Bok et al J Neurosci. 2003 Feb 1;23(3):777-87.

Insert

pkac2

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
mCherry-miniSOG-Paxillin-22

Plasmid

#55092

Purpose

Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Publication

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Paxillin

Tags

mCherry-miniSOG

Expression

Mammalian

Promoter

CMV

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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GFP-Rab30

Plasmid

#31743

Depositor

Ron Vale

Publication

Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

Insert

Rab30 (Rab30 Fly)

Tags

GFP

Expression

Insect

Promoter

Actin

Available since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only
Keratin 8 S431A

Plasmid

#18058

Depositor

M Bishr Omary

Publication

Ku et al J Biol Chem. 2002 Mar 29. 277(13):10775-

Insert

Keratin 8 (KRT8 Human)

Expression

Mammalian

Mutation

S431A

Available since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
M10.2::GFP-ACNF Targeting Vector

Plasmid

#15859

Depositor

Peter Mombaerts

Publication

Ishii et al J Neurosci. 2008 Mar 5. 28(10):2332-41.

Insert

M10.2

Expression

Bacterial

Available since

May 6, 2008

Availability

Academic Institutions and Nonprofits only
pSRG11 (eft-3p::scfv(glo)::gfp(smu-1 introns)::tbb-2 3’UTR)

Plasmid

#245121

Purpose

Optimized SunTag antibody for expression in C. elegans. Useful for visualization of translation of GCN4 encoding mRNAs or for visualization of GCN4-tagged proteins

Depositor

Suzan Ruijtenberg

Publication

van der Salm et al Development. 2025 May 15;152(10):dev2044

Inserts

eft-3p
scFv (GLO)
GFP (GLO)
tbb-2 3'UTR
left recombination arm MosSCI ttTi5605
right recombination arm MosSCI ttTi5605

Expression

Bacterial and Worm

Available since

Jan. 8, 2026

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pX330_H1.0

Plasmid

#247482

Purpose

Expresses SpCas9 and a sgRNA targeting the human histone H1.0 loci for knock-in.

Depositor

Kazuhiro Maeshima

Publication

Shimazoe et al Sci Adv. 2026 Apr 10;12(15):eaec9801. do

Insert

H1.0 (H1F0) (H1-0 Human)

Use

CRISPR

Promoter

U6

Available since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-9-Fzd8

Plasmid

#246709

Purpose

Expression vector for mouse Fzd8, N-terminal 3xFLAG tag

Depositor

Calvin Kuo, Mario Vallon

Publication

Vallon et al Cell Rep. 2018 Oct 9;25(2):339-349.e9. d

Insert

Fzd8 (Fzd8 Mouse)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available since

Jan. 2, 2026

Availability

Academic Institutions and Nonprofits only

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