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PKMC356_pTRE-ActivinA_IRES_BFP_WPRE

Plasmid

#225355

Purpose

Lentiviral vector for ActivinA and BFP expression

Depositor

Wendell Lim

Publication

Yamada et al Cell. 2024 Dec 11:S0092-8674(24)01323-0.

Insert

Actvin A and TagBFP (Inhba Synthetic, Mouse)

Use

Lentiviral

Expression

Mammalian

Available since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF-G1(5CNW)RS

Plasmid

#222872

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of 5-Cyano-L-Tryptophan (5-CN-Trp) into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Publication

Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF-G1(6CNW)RS

Plasmid

#222873

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of 6-Cyano-L-Tryptophan (6-CN-Trp) into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Publication

Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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mTALEAD 16S rRNA-1139T10C

Plasmid

#210838

Purpose

mTALEAD expression vector

Depositor

Shin-ichi Arimura

Publication

Zhou et al Plant Physiol. 2023 Dec 21:kiad678. doi:

Insert

For specific A-to-G base editing to introduce targeted A-to-G mutation in 16S rRNA in chloroplast

Expression

Plant

Available since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-4V5-APEX2-URA

Plasmid

#207056

Purpose

For ORF tagging with 4V5-APEX2

Depositor

Zhiping Xie

Publication

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

4xV5-APEX2

Tags

4xV5-APEX2

Expression

Yeast

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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pRRL.SIN.EF1A.JAG1-B5.GS.CAR-2G

Plasmid

#194463

Purpose

CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.B5 in VH-VL order and (GGGGS)3 linker (scFv). GFP-Zeo for selection and monitoring.

Depositor

Ana Sofia Coroadinha

Publication

Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

Insert

Anti-JAG1 CAR

Use

Lentiviral

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pRRL.SIN.EF1A.JAG1-F1.GS.CAR-2G

Plasmid

#194461

Purpose

CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.F1 in VH-VL order and (GGGGS)3 linker (scFv). GFP-Zeo for selection and monitoring.

Depositor

Ana Sofia Coroadinha

Publication

Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

Insert

Anti-JAG1 CAR

Use

Lentiviral

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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ApoE4_47-300_mCh-SspB (pBS1069)

Plasmid

#185292

Purpose

For the mammalian expression of the human protein ApoE4_47-300 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

Depositor

Pamela Silver

Publication

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

ApoE4_47-300

Expression

Mammalian

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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ApoE2_47-300_mCh-SspB (pBS1068)

Plasmid

#185291

Purpose

For the mammalian expression of the human protein ApoE2_47-300 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

Depositor

Pamela Silver

Publication

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

ApoE2_47-300

Expression

Mammalian

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pST_DHN_AtCor47 (pBS0784)

Plasmid

#185254

Purpose

For the mammalian expression of the Arabidopsis protein DHN_AtCor47. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Publication

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

DHN_AtCor47

Expression

Mammalian

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_SERF2_HUMAN (pBS0751)

Plasmid

#185239

Purpose

For the mammalian expression of the human protein SERF2_HUMAN. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Publication

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SERF2_HUMAN

Expression

Mammalian

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pPBpuro-LDR

Plasmid

#173857

Purpose

Encoding a part of the rapamycin-induced dimerization system.

Depositor

Michiyuki Matsuda

Publication

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

a Lyn11-targeted FRB (LDR)

Expression

Mammalian

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2-hFAM 3B-His

Plasmid

#172192

Purpose

Coding sequence of human FAM3B pCS2 C terminal 6xHis tag used for protein purification from conditioned medium

Depositor

Edward De Robertis

Publication

Zhang et al Proc Natl Acad Sci U S A. 2021 May 18;11

Insert

hFAM 3B (FAM3B Human)

Tags

His

Expression

Mammalian

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_RXRB_Hormone-recep

Plasmid

#109849

Purpose

Protein expression and purification of RXRB_Hormone-recep

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

RXRB_Hormone-recep (RXRB Human)

Expression

Bacterial

Available since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_AP2M1_Adap-comp-sub

Plasmid

#109992

Purpose

Protein expression and purification of AP2M1_Adap-comp-sub

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

AP2M1_Adap-comp-sub (AP2M1 Human)

Expression

Bacterial

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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NCVB-ssGrl1-ESCargo*

Plasmid

#140167

Purpose

Expresses a regulatable secretory cargo (without an ER export signal) for Tetrahymena thermophila

Depositor

Benjamin Glick

Publication

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ssGrl1-DsRed-Express2-FKBP(LV)(C22V)

Use

Other

Promoter

MTT1

Available since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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pMH-HT_ygiF-FL

Plasmid

#127795

Purpose

Expresses full-length ygiF in E. coli cells

Depositor

Michael Hothorn

Publication

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

ygiF (ygiF Escherichia coli)

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pEMS1958

Plasmid

#82569

Purpose

High copy number plasmid containing PR2/3:Con MiniPromoter insert.

Depositor

Elizabeth Simpson

Publication

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

PRS2/3:Con

Use

Pleiades promoter project [sic, pleaides plieades]

Available since

Sept. 14, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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Cav1-miniSOG

Plasmid

#67667

Purpose

Mammalian expression vector driving dog Caveolin1-minSOG

Depositor

Rob Parton

Publication

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Dog Cav1-miniSOG

Expression

Mammalian

Promoter

CMV

Available since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pCMB-TLEr

Plasmid

#61188

Purpose

Fluorescent reporter for transitory late endosome mCherry-MtRAB7A2 expressed under MtBCP1 promoter

Depositor

Maria Harrison

Publication

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtRAB7A2

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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