We narrowed to 5,822 results for: pCas

1. pDL22_Cas9-His Δcys E532C

   Plasmid

   #206283

   Purpose

   Bacterial expression of Cas9-His Δcys E532C variant under T7 promoter

   Depositor

   Amit Choudhary

   Article

   Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   6x-His and SV40 NLS

   Expression

   Bacterial

   Mutation

   C80S, E532C, C574S

   Promoter

   T7

   Available Since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. lenti-Cas9-sgHPRT1

   Plasmid

   #196713

   Purpose

   CRISPR-KO. WT-SpCas9 and sgRNA targeting HPRT1. Editing-competent cells can be selected with 6-TG

   Depositor

   Bernhard Schmierer

   Article

   Schmierer et al Mol Syst Biol. 2017 Oct 9;13(10):945. do

   Insert

   Cas9-T2A-BSD-U6-sgHPRT1
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Promoter

   EF1a/hU6

   Available Since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pC1300_pUB10_pcoCASphi_E9t_MCS_version1

   Plasmid

   #197937

   Purpose

   T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter, with SV40 NLS at the N-terminal and nucleoplasmin NLS at the C-terminal.

   Depositor

   Steven Jacobsen

   Article

   Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

   Insert

   pcoCasphi-2
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   pUBQ10

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. UAS-GFP::NMN-Deamidase

   Plasmid

   #187868

   Purpose

   Gal4/UAS expression of GFP::NMN-Deamidase

   Depositor

   Lukas Neukomm

   Article

   Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

   Insert

   NMN-Deamidase
   

   Tags

   GFP

   Expression

   Insect

   Available Since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. TRE-RARa-VP16

   Plasmid

   #185561

   Purpose

   Doxycycline inducible expression of constitutively active Retinoic Acid Receptor alpha

   Depositor

   Connie Cepko

   Article

   Amamoto et al Elife. 2022 Mar 22;11. pii: 76389. doi:

   Insert

   RARa-VP16 (Rara Mouse)
   

   Tags

   VP16

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   June 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX458_sgRNA_Ago1_5

   Plasmid

   #172470

   Purpose

   Expresses SpCas9, GFP, and sgRNA targeting Ago1

   Depositor

   Constance Ciaudo

   Article

   Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

   Insert

   sgAgo1
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX458_sgRNA_Ago1_6

   Plasmid

   #172471

   Purpose

   Expresses SpCas9, GFP, and sgRNA targeting Ago1.

   Depositor

   Constance Ciaudo

   Article

   Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

   Insert

   sgAgo1
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. BPK617

   Plasmid

   #179297

   Purpose

   Expresses dCas9 fused to VP64

   Depositor

   Keith Joung

   Article

   Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d

   Insert

   dCas9-VP64
   

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically inactive Cas9)

   Promoter

   CAG

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. NAX_CAGGS_CAS9_iRFP713

   Plasmid

   #167860

   Purpose

   PiggyBac compatible plasmid expressing spCas9

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   Cas9
   

   Use

   CRISPR

   Available Since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. GST-TEVcs- FIP200 delta641-779-MBP

    Plasmid

    #171411

    Purpose

    For the purifcation of human ULK1 truncated complex

    Depositor

    James Hurley

    Article

    Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

    Insert

    FIP200 (RB1CC1 Synthetic)
    

    Expression

    Mammalian

    Mutation

    truncation (delta641-779-MBP)

    Available Since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB-TAC-Cas9

    Plasmid

    #120352

    Purpose

    piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry). SpCas9 is constitutively expressed.

    Depositor

    Knut Woltjen

    Article

    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

    Type

    Empty backbone

    Use

    CRISPR; Piggybac transposon

    Expression

    Mammalian

    Promoter

    tet-on and CBh

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pL2-1 ConjEpi PtEF2bsd Pt49202HMGRVenusThrFLAG

    Plasmid

    #139963

    Purpose

    Over-expression of native 3-hydroxy-3-methyl glutaryl CoA reductase

    Depositor

    Raffaela Abbriano

    Article

    Jaramillo-Madrid et al Pharmaceuticals (Basel). 2020 Dec 21;13(

    Insert

    ConjEpi PtEF2_bsd_PttEF2 Pt49202_HMGRVenusThrFLAG_Ptt49202
    

    Use

    Synthetic Biology

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. MTK234_058

    Plasmid

    #123984

    Purpose

    Encodes the spCas9 Safetargeting Chr11 gRNA expression cassette as a type 234 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    sg Safetargeting Chr11
    

    Expression

    Mammalian

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. MTK234_038

    Plasmid

    #123917

    Purpose

    Encodes the spCas9 pSV40 (site 1) gRNA expression cassette as a type 234 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    sgRNA-pSV40
    

    Expression

    Mammalian

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. MTK234_043

    Plasmid

    #123913

    Purpose

    Encodes the spCas9 pSV40 (site 2) gRNA expression cassette as a type 234 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    sgRNA-pSV40
    

    Expression

    Mammalian

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. EED-KO-gRNA-2

    Plasmid

    #131322

    Purpose

    gRNA to knockout EED. Use with EED-KO-gRNA-1

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    EED-KO-gRNA-2
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

17. MTK234_015

    Plasmid

    #123991

    Purpose

    Encodes the spCas9 sgRNA1 hROSA26 (site 4) gRNA expression cassette as a type 234 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    hROSA26 sg4
    

    Expression

    Mammalian

    Available Since

    Feb. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. MTK234_047

    Plasmid

    #124055

    Purpose

    Encodes the spCas9 mScarlet (site 2) gRNA expression cassette as a type 234 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    mScarlet sgRNA 2
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. MTK234_048

    Plasmid

    #124056

    Purpose

    Encodes the spCas9 mScarlet (site 3) gRNA expression cassette as a type 234 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    mScarlet sgRNA 3
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. MTK234_049

    Plasmid

    #124057

    Purpose

    Encodes the spCas9 mScarlet (site 4) gRNA expression cassette as a type 234 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    mScarlet sgRNA 4
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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