We narrowed to 6,763 results for: poly

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pAP14

Plasmid

#186262

Purpose

wgaA (a.k.a expA1) and wgaB (a.k.a expA23), bicistronic, under light light responsive PEL222 promoter and EL222 under constitutively active LacIq promoter

Depositor

Yongku Cho

Article

Pirhanov et al ACS Synth Biol. 2021 Feb 19;10(2):345-35

Inserts

wgaAB bicistronic
EL222 (ELI_04755 Erythrobacter litoralis)

Expression

Bacterial

Promoter

LacIq (CGAATGGTGCAAAACCTTTCGCGGTATGGCATGATAGCGCCC…

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-2T-TRR-C421-G2304D

Plasmid

#182845

Purpose

mutation G2304D (GGC/GAC), PCR product coding C-terminal residues 2011-2431 of TRR was inserted into modified pGEX-2T by Nde I-Nsi I sites

Depositor

Peter Harte

Article

Tie et al Development. 2014 Mar;141(5):1129-39. do

Insert

TRR 2011-2431 (trr Fly)

Tags

GST

Expression

Bacterial

Mutation

mutation G2304D (GGC/GAC)

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-2T-TRX-C751-E3616S

Plasmid

#182844

Purpose

mutation E3616S (GAA/TCA), PCR product coding C-terminal residues 2976-3726 of TRX was inserted into modified pGEX-2T by Nde I-Nsi I sites. Expression in E. coli. by IPTG induction

Depositor

Peter Harte

Article

Tie et al Development. 2014 Mar;141(5):1129-39. do

Insert

TRX (w Fly)

Tags

GST

Expression

Bacterial

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-GST-NQO1(Y128F)

Plasmid

#177141

Purpose

Bacterial vector for expression of an N-terminal GST fusion of NQO1(Y128F) with a TEV protease site located between the GST tag and NQO1(Y128F)

Depositor

Robert Sobol

Article

Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

Insert

NQO1 (NQO1 Human)

Tags

GST

Expression

Bacterial

Promoter

Tac

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pET-DEST42-dre4

Plasmid

#170440

Purpose

Express drosophila dre4 in E.coli expression system. Generated from pDONR-dre4 by Gateway system. Used for custom antibody purification.

Depositor

Joan Conaway

Article

Tettey et al Cell Rep. 2019 Jun 25;27(13):3770-3779.e

Insert

dre4 (dre4 Fly)

Tags

6xHis and V5

Expression

Bacterial

Mutation

Deleted amino acids 1-20

Promoter

T7

Available Since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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CPH3400 (YCp LEU2 Rpb1 A1529_G1534delInsLEVLFQGP)

Plasmid

#91806

Purpose

Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site

Depositor

Chris Hill

Article

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

RPO21 (RPO21 Budding Yeast)

Expression

Yeast

Mutation

Residues A1529-G1534 replaced with a PreScission …

Promoter

Endogenous Rpb1 promoter

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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CPH3477 (YCp LEU2 Rpb1 P1455_E1456InsLEVLFQGP)

Plasmid

#91807

Purpose

Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site

Depositor

Chris Hill

Article

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

RPO21 (RPO21 Budding Yeast)

Expression

Yeast

Mutation

PreScission Protease site (LEVLFQGP) inserted bet…

Promoter

Endogenous Rpb1 promoter

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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CPH3506 (pET151_Spt6 1247-1451 K1355A,K1435A)

Plasmid

#91818

Purpose

Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with K1435A and K1355A mutations

Depositor

Chris Hill

Article

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

SPT6 (SPT6 Budding Yeast)

Tags

6xHis

Expression

Bacterial

Mutation

changed lysine 1355 and lysine 1435 to alanines; …

Promoter

T7

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pLJM1-Flag-Rheb-12Rs

Plasmid

#165023

Purpose

expression of Flag-Rheb-12Rs mutant protein in mammalian cells (all the lysine residues on Rheb except the K19 and K120 are mutated to arginines).

Depositor

Ken Inoki

Article

Yao et al Mol Cell. 2020 Nov 5;80(3):437-451.e6. d

Insert

Rheb-12Rs (RHEB Human)

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

All the lysine residues except K19 and K120 are c…

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pX330_gRNA_H19-DMR-2

Plasmid

#159930

Purpose

Specific gRNA against mouse H19-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of H19-DMR in mouse ESC.

Depositor

Anton Wutz

Article

Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

Insert

gRNA mouse H19-DMR (H19-icr Mouse)

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

U6, CBh

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pX330_gRNA_H19-DMR-1

Plasmid

#159929

Purpose

Specific gRNA against mouse H19-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of H19-DMR in mouse ESC.

Depositor

Anton Wutz

Article

Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

Insert

gRNA mouse H19-DMR (H19-icr Mouse)

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

U6, CBh

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCDF11-SFPQ(214-598)_quadruple_mutant

Plasmid

#135441

Purpose

Expresses SFPQ 214-598 quadruple mutant (L535A, L539A, L546A, M549A) with TEV-cleavable hexahistidine tag

Depositor

Charles Bond

Article

Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

Insert

splicing factor proline and glutamine rich (SFPQ Human)

Tags

Hexahistidine

Expression

Bacterial

Mutation

L535A, L539A, L546A, M549A

Promoter

T7

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pBacMam2-DiEx-LIC-N entry vector

Plasmid

#111751

Purpose

Intermediate vector containing HTT gene, except for polyQ region. Used to clone various polyQ lengths into HTT sequence with N-term FLAG tag. Baculovirus transfer vector for insect and mammalian cells

Depositor

Cheryl Arrowsmith

Article

Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

Type

Empty backbone

Use

Baculovirus expression

Tags

FLAG

Expression

Mammalian

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 Dynamin1 T78R L84R T92R V118R

Plasmid

#112106

Purpose

Mammalian expression plasmid of GFP-tagged Dynamin protein.

Depositor

Justin Taraska

Article

Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

Insert

Dynamin1 (DNM1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

T78R L84R T92R V118R

Promoter

CMV

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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hASXL(1-115)-luciferase

Plasmid

#106427

Purpose

Expresses human ASXL1 (amino acids 1-115) fused to the N-terminal of firefly luciferase

Depositor

Douglas Feldman

Article

Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

Insert

addition sex combs like 1 ASXL1 (ASXL1 Human)

Tags

luciferase

Expression

Mammalian

Mutation

amino acids 1-115 fused to luciferase

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pJC20cdc8.27

Plasmid

#99362

Purpose

Bacterial expression vector containing cDNA encoding for the temperature sensitive fission yeast tropomyosin mutant, Cdc8.27.

Depositor

Dan Mulvihill

Article

Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

Insert

cdc8 (cdc8 Fission Yeast)

Expression

Bacterial

Mutation

Glutamic acid 129 to Lysine

Promoter

T7

Available Since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-GST-CHD5-WT-FRAG

Plasmid

#68873

Purpose

IPTG inducible bacterial expression of CHD5 fragment

Depositor

Adrian Bracken

Article

Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

Insert

CHD5 (CHD5 Human)

Tags

GST

Expression

Bacterial

Mutation

331-672aa

Promoter

tac

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only
pCR2.1-Ins2-842

Plasmid

#53969

Purpose

842 bp insert from mouse Ins2 gene used as a control for methylation-specific PCR assays

Depositor

Kevin Ferreri

Article

Husseiny et al PLoS One. 2012;7(10):e47942. doi: 10.137

Insert

mouse Ins2 gene partial (Ins2 Mouse)

Expression

Bacterial

Promoter

lac

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

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pTU1-A-pdh_RiboJ_mCherry_Bba_B0015

Plasmid

#107581

Purpose

B. megaterium DSM319 pdh promoter, mCherry (Bacillus codon optimised) for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)

Depositor

Paul Freemont

Article

Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p

Insert

mCherry

Use

Synthetic Biology

Expression

Bacterial

Promoter

pdh promoter Bacillus megaterium DSM319

Available Since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

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pGEMHE-mScarlet-hCenpC

Plasmid

#171490

Purpose

T7 promotor drives in vitro transcription of mScarlet-tagged human hCenpC mRNA

Depositor

Melina Schuh

Article

Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

Insert

CenpC (CENPC Human)

Use

Pgehme

Tags

mScarlet

Promoter

T7

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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