We narrowed to 7,565 results for: Tet-on

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pBC114

Plasmid

#202276

Purpose

Level 2 for strain-specific barcode DNA tag with bc141 through Tn7 bacterial insertion, Tetracyline selectable marker and far-red-FP fluorescent marker mPlum

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc141

Expression

Bacterial

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-mRuby3-WPRE

Plasmid

#193343

Purpose

AAV-mediated expression of mRuby3 under the TRE promoter in mammalian cells

Depositor

Takeshi Imai

Article

Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

Insert

mRuby3

Use

AAV

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pFU-MVIIA-PE

Plasmid

#24144

Depositor

Ines Ibanez-Tallon

Article

Auer et al Nat Methods. 2010 Mar;7(3):229-36. doi:

Insert

omega-Conotoxin MVIIA-FLAG-PDGF_R_TM-EGFP (PDGFRB Conus magus, Aequorea victoria, Human)

Use

Lentiviral

Expression

Mammalian

Available Since

April 28, 2010

Availability

Academic Institutions and Nonprofits only

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pUBQ10::ZF108_3xFLAG_YPet

Plasmid

#106441

Purpose

pUBQ10 driven Zinc Finger YPet fusion that targets the FWA promoter in arabidopsis

Depositor

Steven Jacobsen

Article

Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

Insert

ZF108_3xFLAG_YPet

Use

Synthetic Biology

Expression

Plant

Available Since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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pBEAST-pBen-Luc

Plasmid

#122694

Purpose

Output expression of firefly luciferase under the activation of BenR transcription factor for cell-free expression

Depositor

Jerome Bonnet

Article

Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

Insert

Firefly luciferase

Use

Synthetic Biology; Cell-free protein synthesis

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pBEAST-pBen-sfGFP

Plasmid

#114598

Purpose

"Output expression of sfGFP under the activation of BenR transcription factor for cell-free expression"

Depositor

Jerome Bonnet

Article

Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

Insert

sfGFP

Use

Synthetic Biology; Cell-free protein synthesis

Promoter

PBen

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pBEAST-BenR

Plasmid

#114597

Purpose

"Strong constitutive expression of transcription factor, BenR, for cell-free expression"

Depositor

Jerome Bonnet

Article

Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

Insert

BenR

Use

Synthetic Biology; Cell-free protein synthesis

Promoter

OR2-OR1-Pr

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAB085d

Plasmid

#79209

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1) luxAB; Ptet 434cI-TnTBR3-F3

Expression

Bacterial

Promoter

PlacZ-opt (OR1); Ptet

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pGLTR-FP-GFP

Plasmid

#58242

Purpose

GATEWAY-compatible lentiviral RNAi delivery vector; SFFV-driven eGFP marker

Depositor

Stephan Geley

Article

Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi:

Insert

attR1-Cm(R)-CcdB-attR2

Use

Lentiviral and RNAi; Gateway destionation vector

Expression

Mammalian

Promoter

no

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pAB085c

Plasmid

#79208

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1) luxAB; Ptet 434cI-TnTBR3-FL

Expression

Bacterial

Promoter

PlacZ-opt (OR1); Ptet

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pEN12A-P750

Plasmid

#49525

Purpose

Ampicillin resistant; entry clone containing PtetO-4C5G and attL1 and attL2 sites for recombination into a Gateway destination vector at the attR4 and attR1 sites

Depositor

Dirk Schnappinger

Article

Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

Insert

P750

Promoter

P750

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pUC-STITCH

Plasmid

#129535

Purpose

STITCH (Synthetic Toplogical Insulator with TetO for CHromatin-engineering) with homology arms for MYC locus insertion.

Depositor

Hideyuki Okano

Article

Tsujimura et al Elife. 2020 May 5;9. pii: 47980. doi: 10

Insert

STITCH

Use

CRISPR; Donor template

Available Since

Dec. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pFU-cMVIIA-PE

Plasmid

#24147

Depositor

Ines Ibanez-Tallon

Article

Auer et al Nat Methods. 2010 Mar;7(3):229-36. doi:

Insert

loxP-DsRed-loxP-omega-Conotoxin MVIIA-FLAG-PDGF_R_TM-EGFP (PDGFRB Conus magus, Aequorea victoria, Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only

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pXGFPC-5 Pxyl-dcas9 (Spy)

Plasmid

#133316

Purpose

expresses dcas9 from Streptococcus pyogenes; repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xylose locus in Caulobacter crescentus; tetracycline resistance

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

dcas9 (Streptococcus pyogenes)

Use

CRISPR

Promoter

xylose

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pBEAST-J23101-CocE

Plasmid

#114600

Purpose

"Strong constitutive expression of metabolic enzyme, CocE, for cell-free expression"

Depositor

Jerome Bonnet

Article

Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

Insert

CocE

Use

Synthetic Biology; Cell-free protein synthesis

Promoter

J23101

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pBS-TRE-mTagBFP2-WPRE

Plasmid

#193332

Purpose

Expresses mTagBFP2 under the TRE promoter in mammalian cells

Depositor

Takeshi Imai

Article

Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

Insert

mTagBFP2

Expression

Mammalian

Mutation

A XhoI site in the coding sequence is silenced

Promoter

TRE

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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p5A0-T

Plasmid

#92016

Purpose

Expresses TALE targeted to lac operator without TEV cut sites, anhydrotetracycline inducible TEV protease

Depositor

Brian Pfleger

Article

Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do

Inserts

TALE targeting lac operator
Tobacco Etch Virus Protease

Use

TALEN

Tags

5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…

Mutation

S219V

Promoter

J23102 Promoter (from Anderson promoter library) …

Available Since

Jan. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pyc128m

Plasmid

#158778

Purpose

soluble GCaMP expression

Depositor

Jennifer Garrison, Zachary Knight

Article

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

GCaMP6m

Use

AAV

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCIneo-HA-Ago2

Plasmid

#115362

Purpose

Expression vector to produce HA-tagged human Ago2 protein without N-peptide

Depositor

Witold Filipowicz, Ramesh Pillai

Article

Pillai et al RNA. 2004 Oct . 10(10):1518-25.

Insert

Argonaute 2 (AGO2 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pET28b – E. coli RecE residues 564-868

Plasmid

#104534

Purpose

Expresses E. coli RecE residues 564-868

Depositor

Charles Bell

Article

Zhang et al Structure. 2009 May 13;17(5):690-702. do

Insert

RecE residues 564-866 (C-terminal nuclease domain)

Tags

6X-His tag followed by thrombin site

Expression

Bacterial

Promoter

T7

Available Since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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