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pCellFree_G03 YY1

Plasmid

#67082

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

YY1 (YY1 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA-Wnt7B-V5

Plasmid

#35934

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt7B (Wnt7b Human)

Tags

V5

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmMe31B

Plasmid

#21683

Depositor

Elisa Izaurralde

Article

Eulalio et al Mol Cell Biol. 2007 Jun . 27(11):3970-81

Insert

maternal expression at 31B (me31B Fly)

Use

Drosophila expression

Tags

lambdaN-HA

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pAGM9121_pFDH1

Plasmid

#160913

Purpose

Formate dehydrogenase (FDH1) promoter module for episomal P. pastoris promoter shuffling

Depositor

Sylvestre Marillonnet

Article

Pullmann et al ACS Synth Biol. 2021 Jun 2. doi: 10.1021

Insert

formate dehydrogenase promoter

Use

Synthetic Biology

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_pFLD1

Plasmid

#160914

Purpose

Formaldehyde dehydrogenase (FLD1) promoter module for episomal P. pastoris promoter shuffling

Depositor

Sylvestre Marillonnet

Article

Pullmann et al ACS Synth Biol. 2021 Jun 2. doi: 10.1021

Insert

Formaldehyde dehydrogenase promoter

Use

Synthetic Biology

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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IRE-TREAT

Plasmid

#196921

Purpose

The 5UTR of the ferritin heavy chain mRNA was cloned upstream of the Renilla coding sequence in the ΔIRE-TREAT reporter

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Expression

Bacterial and Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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PP363

Plasmid

#78984

Purpose

255B. Promoter: scTEF1, minimal Promoter: GAP. Figure 5. Expresses rHGH and IFNa2b.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Inserts

rHGH
IFNa2b

Expression

Yeast

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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MXS_Tet

Plasmid

#62422

Purpose

MXS_chaining vector with Tet

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Tetracycline inducible promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 SP6

Plasmid

#67074

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

SP6 (SP6 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pJUMP19-Ptac_P

Plasmid

#127004

Purpose

Basic Part P- Promoter (non-constitutive); Ptac; IPTG/lactose induced; controlled by LacI.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part Ptac_P

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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ASPH

Plasmid

#39094

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

ASPH (ASPH Human)

Tags

His Tev

Expression

Bacterial

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
RIT2

Plasmid

#55663

Purpose

expression of mouse RIT2

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RIT2 (Rit2 Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA-Wnt6-V5

Plasmid

#35932

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt6 (WNT6 Human)

Tags

V5

Expression

Mammalian

Mutation

Silent Mutation G (567) --> A

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pSP64-AGS-mCherry

Plasmid

#135596

Purpose

Expression of AGS-mCherry mRNA for microinjection for visualization of AGS protein dynamics in S. purpuratus

Depositor

Mamiko Yajima

Article

Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

Insert

AGS-mCherry

Use

In vitro transcription (mrna synthesis)

Tags

mCherry

Promoter

Unknown

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt9A-V5

Plasmid

#35937

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt9A (WNT9A Human)

Tags

V5

Expression

Mammalian

Mutation

Silent Mutation A (543) --> G

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pET28b-AF0623

Plasmid

#30164

Depositor

SeWon Suh

Article

Kim do et al Acta Crystallogr Sect F Struct Biol Crys

Insert

AF0623 (lig Archaeoglobus fulgidus)

Tags

His

Expression

Bacterial

Available Since

April 19, 2012

Availability

Academic Institutions and Nonprofits only
RASL11A

Plasmid

#55685

Purpose

expression of mouse RASL11A

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RASL11A (Rasl11a Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-p85α_wt

Plasmid

#39496

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

p85 (PIK3R1 Bovine)

Expression

Mammalian

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
MXS_tTA2

Plasmid

#62429

Purpose

MXS_chaining vector with rTA2

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Tetracycline transactivator

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt8B

Plasmid

#35917

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt8B (WNT8B Human)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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