We narrowed to 13,642 results for: NTS

1. pFAB3725

   Plasmid

   #47837

   Purpose

   BIOFAB RFP reporter plasmid for measuring promoter 6 + BCD1 efficiency.

   Depositor

   Drew Endy

   Article

   Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

   Insert

   promoter 6 and BCD1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   see insert

   Available since

   Dec. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pFAB3869

   Plasmid

   #47842

   Purpose

   BIOFAB RFP reporter plasmid for measuring promoter 11 + BCD1 efficiency.

   Depositor

   Drew Endy

   Article

   Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

   Insert

   promoter 11 and BCD1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   see insert

   Available since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. VRER variant

   Plasmid

   #138561

   Purpose

   Expresses human codon-optimized SpCas9-VRER and blasticidin resistance: EFS promoter-VRER-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   VRER
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   D1135V, G1218R, R1335E, T1337R

   Promoter

   EFS

   Available since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. E1b-GFP-Tol2-Gateway dre SE-zic2a S

   Plasmid

   #89388

   Purpose

   Vector for reporter assay of one zebrafish super-enhancer region

   Depositor

   Alena Shkumatava

   Article

   Perez-Rico et al Genome Res. 2017 Feb;27(2):259-268. doi:

   Insert

   zic2a (zic2a Zebrafish)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 15, 2017

   Availability

   Academic Institutions and Nonprofits only

5. DCT.VV.Orf176

   Plasmid

   #12864

   Purpose

   This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

   Depositor

   Jack Bennink, Jon Yewdell

   Article

   Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

   Insert

   VV.Orf176
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2006

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA3.2 mir-1-1 reporter mir16-1 wt

   Plasmid

   #46683

   Purpose

   Expresses wild-type miR-16-1 in mammalian cells

   Depositor

   David Bartel

   Article

   Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

   Insert

   mir16-1 wt (MIR16-1 Human, Synthetic)
   

   Use

   RNAi

   Tags

   V5 and hsa-mir-1-1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. DCT.VV.Orf222

   Plasmid

   #12910

   Purpose

   This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

   Depositor

   Jack Bennink, Jon Yewdell

   Article

   Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

   Insert

   VV.Orf222
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 29, 2006

   Availability

   Academic Institutions and Nonprofits only

8. DCT.VV.Orf264

   Plasmid

   #12952

   Purpose

   This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

   Depositor

   Jack Bennink, Jon Yewdell

   Article

   Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

   Insert

   VV.Orf264
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 29, 2006

   Availability

   Academic Institutions and Nonprofits only

9. DCT.VV.Orf194

   Plasmid

   #12882

   Purpose

   This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

   Depositor

   Jack Bennink, Jon Yewdell

   Article

   Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

   Insert

   VV.Orf194
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2006

   Availability

   Academic Institutions and Nonprofits only

10. pmalc4x_E323K_TEV

    Plasmid

    #47699

    Purpose

    Expresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation E323K

    Depositor

    Raquel Lieberman

    Article

    Burns et al Biochemistry. 2011 Jul 5;50(26):5824-33.

    Insert

    Maltose Binding Protein and Olfactomedin-like domain with TEV cleavage site (MYOC Human)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    changed glutamic acid 323 to lysine

    Promoter

    Ptac

    Available since

    Sept. 9, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. KHBD00120

    Plasmid

    #34274

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG3956 (sna Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pGEX-4T2 SHP2 D61A

    Plasmid

    #8323

    Depositor

    Ben Neel

    Article

    O'Reilly et al Mol Cell Biol 2000 Jan;20(1):299-311.

    Insert

    SHP2 (PTPN11 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    D61A (activated mutant)

    Promoter

    Available since

    June 17, 2005

    Availability

    Academic Institutions and Nonprofits only

13. pX330-sgRNA_Dicer_3

    Plasmid

    #68809

    Purpose

    specific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of Dicer1 knockout mESCs.

    Depositor

    Constance Ciaudo

    Article

    Bodak et al FEBS Open Bio. 2017 Jan 11;7(2):204-220.

    Insert

    sgRNA mouse Dicer1
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJG488

    Plasmid

    #91196

    Purpose

    WDV replicon T-DNA for gene targeting in wheat scutella, H840A double nickase (nTaCas9_H840A+gUbi8+gUbi1+donor)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    nTaCas9_H840A+gUbi8+gUbi1+donor
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. FusionRed-Sit-N-15

    Plasmid

    #56133

    Purpose

    Localization: Golgi TGN, Excitation: 580, Emission: 608

    Depositor

    Michael Davidson

    Article

    Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

    Insert

    Sit (ST6GAL1 Human)
    

    Use

    Tags

    FusionRed

    Expression

    Mammalian

    Mutation

    aa 1-45 of SiT

    Promoter

    CMV

    Available since

    April 1, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

16. DCT.VV.Orf92

    Plasmid

    #12780

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jack Bennink, Jon Yewdell

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf92
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pAGHa-SERBP1(189-400)

    Plasmid

    #172315

    Purpose

    Expression of the C-terminal (189-400) domain of SERBP1 in E. coli.

    Depositor

    David Libich

    Article

    Baudin et al Biomol NMR Assign. 2021 Oct;15(2):461-46

    Insert

    Plasminogen Activator Inhibitor 1 RNA-binding protein, residues 189-400 (SERBP1 Human)
    

    Use

    Tags

    His8

    Expression

    Bacterial

    Mutation

    deleted 1-188, 401-408

    Promoter

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. p35S::IRE1a

    Plasmid

    #135232

    Purpose

    constitutive expression of IRE1a from A. thaliana (AT2G17520; upregulates UPR signaling)

    Depositor

    Armin Djamei

    Article

    Alcantara et al Plant Methods. 2020 Jan 21;16:4. doi: 10

    Insert

    IRE1a (IRE1A Mustard Weed)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pnEA-vH_His-TEV-DSep1

    Plasmid

    #174493

    Purpose

    bacterial expression of Drosophila Sep1

    Depositor

    Manos Mavrakis

    Article

    Mavrakis et al Nat Cell Biol. 2014 Apr;16(4):322-34. do

    Insert

    Sep1 (Sep1 Fly)
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFAB1935

    Plasmid

    #47860

    Purpose

    BIOFAB reporter plasmid for measuring his_T_min_linker_crp_T_min termination efficiency

    Depositor

    Drew Endy

    Article

    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

    Insert

    his_T_min_linker_crp_T_min double terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    concatenate of his_min and crp_min

    Promoter

    pLTetO15

    Available since

    Dec. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAT9750-BEAR-mCherry

    Plasmid

    #162990

    Purpose

    BEAR target plasmid with split mCherry and disrupted 5' splice site

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    mCherry split with an intron between amino acids 119-120
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAT15416-BEAR-mScarlet-target-BFP

    Plasmid

    #162996

    Purpose

    plasmid expressing an sgRNA targeting the BEAR-mScarlet plasmid along with a TagBFP marker

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    sgRNA targeting the BEAR-mScarlet plasmid
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDGB3 alpha1 P35S:AsCas12a:T35S (GB1440)

    Plasmid

    #160610

    Purpose

    Transcriptional unit for the expression of the AsCas12a in plant systems driven by the CaMV35s promoter.

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

    Insert

    P35s:AsCas12a:T35s
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAT-9222

    Plasmid

    #124222

    Purpose

    Cloning plasmid for EGxFP assay with self-targeting gRNA-s

    Depositor

    Ervin Welker

    Article

    Talas et al Nucleic Acids Res. 2021 Jan 15. pii: 610

    Insert

    self-targeting gRNA between EGFP halves
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pX330-sgRNA_Dicer_2

    Plasmid

    #68808

    Purpose

    specific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of

    Depositor

    Constance Ciaudo

    Article

    Bodak et al FEBS Open Bio. 2017 Jan 11;7(2):204-220.

    Insert

    sgRNA mouse Dicer1
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMOD_C2519

    Plasmid

    #91088

    Purpose

    Module C, Promoter: OsU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    OsU3

    Available since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. KHBD00638

    Plasmid

    #39705

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG3090 (Sox14 Fly)
    

    Use

    Gateway donr vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Aug. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

28. KHBD00118

    Plasmid

    #34272

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG7345 (Sox21a Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

29. 625_HOXD13 in pmiRGlo

    Plasmid

    #78129

    Purpose

    luciferase reporter for miRNA activity

    Depositor

    Heidi Schwarzenbach

    Article

    Eichelser et al Oncotarget. 2014 Oct 30;5(20):9650-63.

    Insert

    HOXD13 (HOXD13 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    3'UTR containing miRNA binding sites

    Promoter

    PGK

    Available since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCTCON2-BXG-2TAG

    Plasmid

    #158138

    Purpose

    Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (2 TAG construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    BFP-GFP fragment
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Cloned in a TAG codon at the first serine residue…

    Promoter

    GAL1-10

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge