We narrowed to 42,612 results for: cha

1. pET151-D-TOPO-PARP14-SΔK

   Plasmid

   #216658

   Purpose

   Expresses multidomain construct of PARP14 with internal SUMO domain (PARP14-SΔK) in bacterial cells.

   Depositor

   Herwig Schüler

   Article

   Chatzicharalampous et al Protein Expr Purif. 2024 Aug 16:106580.

   Insert

   PARP14-SΔK DNA sequence (PARP14 G784-K1808 with internal SUMO-TEV sequence) (PARP14 Synthetic, Human)
   

   Use

   Tags

   6xHis-V5-TEV

   Expression

   Bacterial

   Mutation

   Deletion starting from Q1390 to D1448 and substit…

   Promoter

   T7

   Available since

   Aug. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFA6a-His3MX6-PGAL1

   Plasmid

   #41607

   Depositor

   John Pringle

   Article

   Longtine et al Yeast. 1998 Jul;14(10):953-61.

   Insert

   GAL1 promoter (GAL1 Budding Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   A. gossypii translation elongation factor 1a gene…

   Expression

   Mutation

   Promoter

   Available since

   Dec. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFA6a-kanMX6-PGAL1-GST

   Plasmid

   #41611

   Depositor

   John Pringle

   Article

   Longtine et al Yeast. 1998 Jul;14(10):953-61.

   Insert

   GAL1 promoter (GAL1 Budding Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   A. gossypii translation elongation factor 1a gene…

   Expression

   Mutation

   Promoter

   Available since

   Dec. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFA6a-kanMX6-PGAL1-GFP

   Plasmid

   #41614

   Depositor

   John Pringle

   Article

   Longtine et al Yeast. 1998 Jul;14(10):953-61.

   Insert

   GAL1 promoter (GAL1 Budding Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   A. gossypii translation elongation factor 1a gene…

   Expression

   Mutation

   Promoter

   Available since

   Dec. 11, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. HM3C NaVAe1_p pET24b

   Plasmid

   #127854

   Purpose

   Bacterial expression vector for NaVAe1 bacterial sodium channel residues 143-288. It expresses HIStag-MBP-3C-channel protein.

   Depositor

   Dan Minor

   Article

   Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

   Insert

   NaVAe1p
   

   Use

   Tags

   3C Cleavage site, His, and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRPL28(K55M) SpHIS5 (pNTI666)

   Plasmid

   #115436

   Purpose

   Expresses K55M RPL28 from HIS3 yeast plasmid

   Depositor

   Nicholas Ingolia

   Article

   McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

   Inserts

   RPL28 (RPL28 Budding Yeast)
   HIS5
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   K55M; MfeI site in intron

   Promoter

   Ashbya gospii TEF1 and endogenous

   Available since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJT11

   Plasmid

   #98308

   Purpose

   Overexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Citrus limon limonene synthase LIS1 under the control of GAL1/GAL2 promoter

   Depositor

   Claudia Vickers

   Article

   Peng et al Metab Eng. 2018 Feb 19. pii: S1096-7176(

   Insert

   TRPL3ClLIS1>TRPL41B
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFA6a-His3MX6-PGAL1-3HA

   Plasmid

   #41610

   Depositor

   John Pringle

   Article

   Longtine et al Yeast. 1998 Jul;14(10):953-61.

   Insert

   GAL1 promoter (GAL1 Budding Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   3xHA, A. gossypii translation elongation factor 1…

   Expression

   Mutation

   Promoter

   Available since

   Dec. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. HpMscS

   Plasmid

   #78310

   Purpose

   H. pylori MscS with N-Term 6x Hisitidine Tag and C-Term FLAG Tag

   Depositor

   Douglas Rees

   Article

   Lai et al Protein Sci. 2013 Apr;22(4):502-9. doi:

   Insert

   Mechanosensitive channel of small conductance
   

   Use

   Tags

   6x His Tag and FLAG Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   June 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pIRTetR-GAL80

    Plasmid

    #185853

    Purpose

    Plugging the mechanism of tetracycline-mediated repression of GAL promoters in Saccharomyces cerevisiae

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    gal80Arm1-PAgTEF1-KanMX4-TAgTEF1-PHAC1>TetR>TUP1>PABF1- PTEF1+[TetO]>GAL80Arm2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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