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We narrowed to 23,602 results for: CRISPR

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Showing: 7111 - 7140 of 23602 results

  8. pShHELIX_sgRNA_entry (CJT32)

    Plasmid
    #181782
    Purpose
    Expresses ShHELIX containing a nicking I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    181782-plasmid-map-sequence-id-354808

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  15. pYPQAT09

    Plasmid
    #223381
    Purpose
    T-DNA vector for SpRY mediated mutagenesis for dicot plants; NG or NA PAM preference; SpRY was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    2x35s-SpRY-AtU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223381-plasmid-map-sequence-id-453508

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  17. pTC217

    Plasmid
    #70018
    Purpose
    Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 1b
    Depositor
    Daniel Voytas
    Article
    Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:
    Insert
    Nuclease (Cas9/sgRNA) + Donor + GVR
    Use
    CRISPR
    Expression
    Plant
    Available Since
    March 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    70018-plasmid-map-sequence-id-124557

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  19. pMEDS

    Plasmid
    #236568
    Purpose
    Introduces a multiple expression and dual selection (MEDS) system to use alongside CRISPR for applications such as gene editing.
    Depositor
    Shannon Moonah
    Article
    Sharma et al PLoS One. 2025 May 6;20(5):e0321881. doi
    Inserts
    CD/UPRT (negative selection)
    NPT II (positive selection)
    Tags
    3x Myc and FLAG
    Expression
    Mammalian
    Available Since
    June 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    236568-plasmid-map-sequence-id-477092

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  30. pYPQAT10

    Plasmid
    #223382
    Purpose
    T-DNA vector for SpRY mediated mutagenesis for monocot plants; NG or NA PAM preference; SpRY was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-SpRY-OsU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223382-plasmid-map-sequence-id-453506

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Showing: 7111 - 7140 of 23602 results