We narrowed to 42,658 results for: cha

1. pET28a-ybbr-XMod-Doc (WT, S199AzF)-HIS

   Plasmid

   #153443

   Purpose

   E. coli expression (amber suppression) of Rc. XDocB with serine at position 199 replaced with amber codon.

   Depositor

   Michael Nash

   Article

   Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

   Insert

   Rc.XDocB
   

   Use

   Tags

   6xHis and ybbr tag

   Expression

   Bacterial

   Mutation

   Serine 199 was mutated to amber codon (TAG)

   Promoter

   T7 promoter

   Available since

   July 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMH-HT_ygiF-TTM

   Plasmid

   #127794

   Purpose

   Expresses the TTM/CYTH domain of ygiF in E. coli cells

   Depositor

   Michael Hothorn

   Article

   Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

   Insert

   ygiF (ygiF Escherichia coli)
   

   Use

   Tags

   6xHis tag + TEV cleavage site

   Expression

   Bacterial

   Mutation

   N-terminal TTM domain of ygiF (residues 1-200)

   Promoter

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRPL28 SpHIS5 (pNTI663)

   Plasmid

   #115433

   Purpose

   Expresses wild-type RPL28 from HIS3 yeast plasmid

   Depositor

   Nicholas Ingolia

   Article

   McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

   Inserts

   RPL28 (RPL28 Budding Yeast)
   HIS5
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   MfeI site in intron

   Promoter

   Ashbya gospii TEF1 and endogenous

   Available since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRPL28(Q38P) SpHIS5 (pNTI664)

   Plasmid

   #115434

   Purpose

   Expresses Q38P RPL28 from HIS3 yeast plasmid

   Depositor

   Nicholas Ingolia

   Article

   McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

   Inserts

   RPL28 (RPL28 Budding Yeast)
   HIS5
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Q38P; MfeI site in intron

   Promoter

   Ashbya gospii TEF1 and endogenous

   Available since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRPL28(H39L) SpHIS5 (pNTI665)

   Plasmid

   #115435

   Purpose

   Expresses H39L RPL28 from HIS3 yeast plasmid

   Depositor

   Nicholas Ingolia

   Article

   McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

   Inserts

   RPL28 (RPL28 Budding Yeast)
   HIS5
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   H39L; MfeI site in intron

   Promoter

   ashbya gospii TEF1 and endogenous

   Available since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFA6a-His3MX6-PGAL1-GST

   Plasmid

   #41613

   Depositor

   John Pringle

   Article

   Longtine et al Yeast. 1998 Jul;14(10):953-61.

   Insert

   GAL1 promoter (GAL1 Budding Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   A. gossypii translation elongation factor 1a gene…

   Expression

   Mutation

   Promoter

   Available since

   Dec. 11, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJC25R1+4stop

   Plasmid

   #17723

   Depositor

   Susan Lindquist

   Article

   Dong et al J Biol Chem. 2007 Nov 23. 282(47):34204-

   Insert

   Sup35R1+4 (SUP35 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   E.coli expression plasmid for NM-region of Sup35 …

   Promoter

   Available since

   April 4, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. FLAG-SpELL pNMT-TOPO

   Plasmid

   #15315

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Banks et al J Biol Chem. 2007 Feb 23. 282(8):5761-9.

   Insert

   eleven nineteen lysine rich in leukemia (ell1 Fission Yeast)
   

   Use

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

9. FLAG-SpEAF pNMT-TOPO

   Plasmid

   #15316

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Banks et al J Biol Chem. 2007 Feb 23. 282(8):5761-9.

   Insert

   ELL associated factor (eaf1 Fission Yeast)
   

   Use

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

10. FLAG-SpELL pBacPAK8

    Plasmid

    #15317

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Banks et al J Biol Chem. 2007 Feb 23. 282(8):5761-9.

    Insert

    eleven nineteen lysine rich in leukemia (ell1 Fission Yeast)
    

    Use

    Baculovirus construction

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only