We narrowed to 11,122 results for: ARIA

1. plenti-CAG-IKZF2-V2-FLAG-IRES-GFP

   Plasmid

   #69049

   Purpose

   lentiviral expression of IKZF2 variant 2 with FLAG tag

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   IKZF2 (IKZF2 Human)
   

   Use

   Lentiviral

   Tags

   FLAG and IRES-GFP

   Expression

   Mammalian

   Mutation

   splice variant 2

   Promoter

   CAG

   Available since

   Oct. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV.CAG.SF-Venus-iGluSnFR.A184V

   Plasmid

   #106202

   Purpose

   Medium affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

   Insert

   SF-Venus-iGluSnFR.A184V
   

   Use

   AAV

   Tags

   Expression

   Mutation

   SFGFP: T203Y, F46L, T65G, S72A GltI: A184V

   Promoter

   CAG

   Available since

   Feb. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAG-hFnCas12a-NLS(nucleoplasmin)-3xHA (AAS1472)

   Plasmid

   #114089

   Purpose

   CAG promoter expression plasmid for human codon optimized FnCas12a nuclease with C-terminal NLS and HA tag

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized FnCas12a
   

   Use

   Tags

   NLS(nucleoplasmin) and 3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPB-cT3G-cERP2-ELK1

   Plasmid

   #192918

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of ELK1

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   ELK1 (ELK1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   TET3G-miniCMV

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLV-TRE3G_3xFLAG-PGC1a4_CMV-mCherry:T2A:Hygro

   Plasmid

   #154263

   Purpose

   Dox/Tet-inducible expression of the Human PGC-1a4 splice variant in mammalian cells.

   Depositor

   Antonio Amelio

   Article

   Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

   Insert

   3xFLAG-PGC-1a4 splice variant (PPARGC1A Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDONR223_RNF212_WT

   Plasmid

   #81860

   Purpose

   Gateway Donor vector containing RNF212 , part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, Jesse Boehm, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   RNF212 (RNF212 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   W4L

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTE4889

   Plasmid

   #88905

   Purpose

   Expresses FnCpf1 crRNA and inactive/dead, humanized FnCpf1 nuclease

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Inserts

   Fn crRNA
   hFnCpf1(D917A)
   

   Use

   CRISPR

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and human U6

   Available since

   April 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDONR223_PPP6C_p.E180K

   Plasmid

   #81334

   Purpose

   Gateway Donor vector containing PPP6C , part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, Jesse Boehm, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   PPP6C (PPP6C Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   E180K

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-enCas12a-A3A-Y130F

   Plasmid

   #193652

   Purpose

   Expression of base editor enCas12a-A3A-Y130F in mammalian cells for base editing, along with non-fused EGFP fluorescence

   Depositor

   Liangxue Lai

   Article

   Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

   Insert

   dLb-enCas12a-hA3A-Y130F, EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   dLbCas12a-D156R/G532R/K538R, hAPOBEC3A-Y130F

   Promoter

   CMV

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCC_06 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9NG-NLS-VPR-2A-Puro-WPRE

    Plasmid

    #139091

    Purpose

    Expresses human codon-optimized inactive SpCas9-NG fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    dSpCas9-NG-VPR
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A, L1111R, D1135V,G1218R, E1219F, A1322…

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTwist-SARS-CoV-2 Δ18 K417N+E484K+N501Y

    Plasmid

    #169469

    Purpose

    Encodes SARS-CoV-2 K417N+E484K+N501Y Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

    Insert

    SARS-CoV-2 K417N+E484K+N501Y Spike truncated to remove 18 amino acids (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET-FLAG-SpCas9-HF1-plus

    Plasmid

    #126775

    Purpose

    Expression of increased fidelity SpCas9-HF1-plus in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    SpCas9-HF1-plus
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, MBP, and NLS

    Expression

    Bacterial

    Mutation

    N497A; Q695A; Q926A; amino acids 1005-1013 replac…

    Promoter

    T7

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1 SARS-CoV-2 S D614-trunc

    Plasmid

    #158076

    Purpose

    Expresses SARS-CoV-2 spike protein with a truncation

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    SARS-CoV-2 spike (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV.CAG.SF-Venus-iGluSnFR.S72A

    Plasmid

    #106203

    Purpose

    Weak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-Venus-iGluSnFR.S72A
    

    Use

    AAV

    Tags

    Expression

    Mutation

    SFGFP: T203Y, F46L, T65G, S72A GltI: S72A

    Promoter

    CAG

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV.GFAP.SF-Venus-iGluSnFR.A184S

    Plasmid

    #106195

    Purpose

    Tight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-Venus-iGluSnFR.A184S
    

    Use

    AAV

    Tags

    Expression

    Mutation

    SFGFP: T203Y, F46L, T65G, S72A GltI: A184S

    Promoter

    GFAP

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDONR223_STK11_WT

    Plasmid

    #82320

    Purpose

    Gateway Donor vector containing STK11 ,part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Jesse Boehm, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    STK11 (STK11 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDONR223_SGK1_WT

    Plasmid

    #81834

    Purpose

    Gateway Donor vector containing SGK1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Jesse Boehm, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    SGK1 (SGK1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDONR223_WNT2_WT

    Plasmid

    #81775

    Purpose

    Gateway Donor vector containing WNT2 , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Jesse Boehm, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    WNT2 (WNT2 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPB-cT3G-cERP2-FOSB

    Plasmid

    #192901

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of FOSB

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    FOSB (FOSB Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    TET3G-miniCMV

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDONR223_NFKBIB_WT

    Plasmid

    #82219

    Purpose

    Gateway Donor vector containing NFKBIB , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Jesse Boehm, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    NFKBIB (NFKBIB Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDONR223_WNT5A_WT

    Plasmid

    #82240

    Purpose

    Gateway Donor vector containing WNT5A , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Jesse Boehm, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    WNT5A (WNT5A Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGEX-6P-1-HRD1 aa 236-444

    Plasmid

    #185349

    Purpose

    RING domain-containing GST fusion protein for ubiquitination assays.

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    HRD1/SYVN (SYVN1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 236-444 only

    Promoter

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. LV_EF1a_Stat3(A662C,N664C,V667L)-P2A-Hygro_Barcode

    Plasmid

    #170254

    Purpose

    Barcoded lentiviral vector to express Stat3 (A662C, N664C, V667L) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    STAT3 (STAT3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    A662C, N664C, V667L

    Promoter

    EF1a

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDONR223_SV2C_WT

    Plasmid

    #81924

    Purpose

    Gateway Donor vector containing SV2C, part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Jesse Boehm, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    SV2C (SV2C Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    F346S

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pPGK-T7/2-CD44v3 (-cyt)

    Plasmid

    #137813

    Purpose

    expression of CD44 proteins in mammalian cells

    Depositor

    Ursula Gunthert

    Article

    Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

    Insert

    CD44v3 (-cyt) (CD44 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    without cytoplasmic region, N280D in the V3 region

    Promoter

    PGK

    Available since

    June 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pTE4566

    Plasmid

    #88904

    Purpose

    Expresses MbCpf1 crRNA and inactive/dead, humanized MbCpf1 nuclease

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    Mb crRNA
    hMbCpf1(D986A)
    

    Use

    CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and human U6

    Available since

    April 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pS6-TGFB2-STREP

    Plasmid

    #128503

    Purpose

    Expresses human pro-TGFβ2 (from L21 to S414) in mammalian cells. With N-t STREP(2x) tag.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

    Insert

    TGFB2 (TGFB2 Human)
    

    Use

    Tags

    STREP (2X) tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCC_10 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dCas9NG-NLS-2A-Puro-WPRE

    Plasmid

    #139095

    Purpose

    Expresses human codon-optimized inactive SpCas9-NG fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    KRAB-dSpCas9-NG
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A, L1111R, D1135V,G1218R, E1219F, A1322…

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. ACTB-(A)3x

    Plasmid

    #112057

    Purpose

    ACTB mRNA tagged with 3 copies of Riboglow (variant A)

    Depositor

    Amy Palmer

    Article

    Braselmann et al Nat Chem Biol. 2018 Jul 30. pii: 10.1038

    Insert

    ACTB (ACTB Human)
    

    Use

    Tags

    Riboglow RNA tag

    Expression

    Mammalian

    Mutation

    3 copies of Riboglow tag (variant A) after stop c…

    Promoter

    CMV

    Available since

    June 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. ACTB-(A)2x

    Plasmid

    #112056

    Purpose

    ACTB mRNA tagged with 2 copies of Riboglow (variant A)

    Depositor

    Amy Palmer

    Article

    Braselmann et al Nat Chem Biol. 2018 Jul 30. pii: 10.1038

    Insert

    ACTB (ACTB Human)
    

    Use

    Tags

    Riboglow RNA tag

    Expression

    Mammalian

    Mutation

    2 copies of Riboglow tag (variant A) after stop c…

    Promoter

    CMV

    Available since

    June 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge