We narrowed to 5,566 results for: donor

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pGS96_EGFP(A206K)-I3-01 AAVS donor

Plasmid

#211865

Purpose

I3-01 transgene integration at AAVS1 safe harbor locus

Depositor

Iain Cheeseman

Insert

I3-01

Use

CRISPR

Mutation

N/A

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pDonor-Prf1-ires-mCherry

Plasmid

#209073

Purpose

Targeting vector for the mouse Prf1 locus to correct prf1 gene

Depositor

Klaus Rajewsky

Article

Li et al Sci Immunol. 2024 Feb 2;9(92):eadi0042.

Insert

Prf1-ires-mCherry HDR template (parts of Prf1 with mutated PAM) (Prf1 Mouse)

Use

CRISPR

Available Since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

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pDonor-PRF1-T2A-cDNA

Plasmid

#209076

Purpose

Targeting vector for the human PRF1 locus to replace exon 3 with repaired cDNA

Depositor

Klaus Rajewsky

Article

Li et al Sci Immunol. 2024 Feb 2;9(92):eadi0042.

Insert

PRF1-T2A-cDNA HDRT (PRF1 Human)

Use

CRISPR

Available Since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

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MTOR-F2108L_DARIC(1)_CD19-scFv_AAV6_Donor

Plasmid

#211905

Purpose

Vector for AAV6 donor production. Targeted CD19-scFv DARIC transgene integration to the MTOR locus with the dominant rapamycin resistance MTOR-F2108L mutation via homology-directed repair.

Depositor

Yannick Doyon

Article

Levesque et al bioRxiv 2023.09.14.557485

Insert

DARIC-CD19-scFv

Use

AAV

Expression

Mammalian

Promoter

hPGK1

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pJMC-L2-Reverse-Donor-BsaI

Plasmid

#197843

Purpose

Donor vector to restore L2 destination via BsaI (reverse orientation)

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

None

Use

Unspecified

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pJMC-L2-Reverse-Donor-Esp3I

Plasmid

#197844

Purpose

Donor vector to restore L2 destination via Esp3I (reverse orientation)

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

None

Use

Unspecified

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pminiT-MED6-mEmerald donor

Plasmid

#197865

Purpose

Donor plasmid used for building MED6-mEmerald knock-in mESCs

Depositor

James Zhe Liu

Article

Dong et al bioRxiv 2022.10.26.513917

Insert

Med6 (Med6 Mouse)

Use

Mouse Targeting

Tags

mEmerald

Promoter

NA

Available Since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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pminiT-mEmerald-MED1 donor

Plasmid

#197867

Purpose

Donor plasmid used for building mEmerald-MED1 knock-in mESCs

Depositor

James Zhe Liu

Article

Dong et al bioRxiv 2022.10.26.513917

Insert

CRSP210 (Med1 Mouse)

Use

Mouse Targeting

Tags

mEmerald

Promoter

NA

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pBlueScript II SK-SNAP donor for Hist1h3g

Plasmid

#186932

Purpose

Genomic targeting of SNAP tag at Hist1h3g C-terminal

Depositor

Zhiguo Zhang

Article

Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

Insert

SNAP donor for Hist1h3g (H3c8 Mouse)

Tags

SNAP

Expression

Mammalian

Promoter

T7

Available Since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pMiniT:myc-GluA2 TKIT donor

Plasmid

#169423

Purpose

Donor DNA for TKIT knockin of Myc to GluA2 in mouse

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Myc (Myc Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pMC_cherry donor_Rosa target (pE002)

Plasmid

#149348

Purpose

Replace donor vector for cherry insertion using the mouse Rosa26 guide RNA

Depositor

Ralf Kuehn

Article

Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

Insert

cherry

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pMC_cherry donor_Polb target (pE038)

Plasmid

#149349

Purpose

Replace donor vector for cherry insertion using the human Polb guide RNA

Depositor

Ralf Kuehn

Article

Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

Insert

cherry

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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HoxC-downstream-5xTetO Donor

Plasmid

#131341

Purpose

Donor template to insert 5xTet operator sequence into downstream of HoxC cluster.

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

HoxC-downstream-5xTetO Donor

Use

CRISPR and Mouse Targeting

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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mGDF8 donor CFP-hDF

Plasmid

#72590

Purpose

Plasmid contains left and right homology arms for mouse GDF8 flanking the mCherry-puromycin-2A-ECFP-dysferlin cassette.

Depositor

Renzhi Han

Article

Xu et al Mol Ther Nucleic Acids. 2013 Jul 30;2:e1

Insert

dysferlin (DYSF Human)

Tags

ECFP

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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hGDF8 donor CFP-hDF

Plasmid

#72591

Purpose

Plasmid contains left and right homology arms for human GDF8 flanking the mCherry-puromycin-2A-ECFP-dysferlin cassette.

Depositor

Renzhi Han

Article

Xu et al Mol Ther Nucleic Acids. 2013 Jul 30;2:e1

Insert

dysferlin (DYSF Human)

Tags

ECFP

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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pDonor-tBFP-NLS-Neo (Universal)

Plasmid

#80767

Purpose

Universal donor vector for CRISPR/Cas9-mediated homology-independent knock-in system.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

Insert

PITCh-gRNA#3 targeting sequence (GCATCGTACGCGTACGTGTT)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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peSpCas9(1.1)-2×sgRNA (IFT88, donor)

Plasmid

#80769

Purpose

Expresses eSpCas9(1.1) and two sgRNAs. The first gRNA targets human IFT88 and the second gRNA targets a pDonor-tBFP-NLS-Neo (Universal).

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

Insert

IFT88 gRNA#1 (IFT88 Human)

Use

CRISPR

Expression

Mammalian

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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R26-H2B-EGFP HR donor vector

Plasmid

#137925

Purpose

R0SA26 knock-in vector with homologous recombination-based method

Depositor

Hiroshi Kiyonari

Article

Abe et al Cell Rep. 2020 May 19;31(7):107653. doi:

Insert

SA-H2B-EGFP-bpA with homology arms

Use

Mouse Targeting

Available Since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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pX458-Dual-Guide-Donor-ShuttleOnly

Plasmid

#171098

Purpose

Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Type

Empty backbone

Use

CRISPR

Mutation

XbaI site added upstream of U6 promotor. GtoC mu…

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pEF1a-donor_opt(R2Tg)-GFP(intron)

Plasmid

#223252

Purpose

Mammalian expression of the optimized R2Tg RNA donor

Depositor

Wei Li

Article

Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

Insert

R2Tg donor_opt

Expression

Mammalian

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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