We narrowed to 5,514 results for: donor

1. Bxb1-GA_AIO-TetOn_Down-Tandem Donor

   Plasmid

   #229785

   Purpose

   Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression (empty)

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   LacZ; rtTA-T2A-mTagBFP2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE and CAG

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX458-Dual-Guide-Donor-ShuttleOnly

   Plasmid

   #171098

   Purpose

   Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   XbaI site added upstream of U6 promotor. GtoC mu…

   Promoter

   Available since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTRE-TIGHT-EGFP-donor fw copy

   Plasmid

   #22074

   Depositor

   Rudolf Jaenisch

   Article

   Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

   Insert

   TETO-eGFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKnockinDonor-human-p16INK4A-P2A-mCherry

   Plasmid

   #137026

   Purpose

   donor vector to knock in P2A-mCherry to the C-terminus of endogenous human p16INK4A

   Depositor

   Chunliang Li

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2019 Dec 9. pi

   Insert

   p16INK4A-knockin-homology arm-P2A-mCherry
   

   Use

   Tags

   P2A-mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   no promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. peSpCas9(1.1)-2×sgRNA (empty, donor)

   Plasmid

   #80768

   Purpose

   All-in-one vector for CRISPR/Cas9-mediated homology-independent knock-in system. The plasmid contains eSpCas9(1.1) and two sgRNA expression cassettes. The first gRNA cloning site is empty.

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. Bxb1-GA_AIO-GZV-SynZiFTR_mNgn2 donor

   Plasmid

   #229789

   Purpose

   Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one grazoprevir-inducible expression of mouse neurogenin 2

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   mNgn2; ZF10-NS3-p65 (Neurog2 Mouse)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   ZF10 and CAG

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. Puro-T2A-mCitrine-hSmad2-donor

   Plasmid

   #203663

   Purpose

   Donor plasmid for CRISPR knock-in

   Depositor

   Ali Hemmati Brivanlou

   Article

   Yoney et al Elife. 2018 Oct 12;7:e38279. doi: 10.755

   Insert

   Smad2
   

   Use

   Tags

   mCitrine

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. BSD-P2A-TagRFP-hSmad1-donor

   Plasmid

   #203662

   Purpose

   Donor plasmid for CRISPR knock-in

   Depositor

   Ali Hemmati Brivanlou

   Article

   Yoney et al Elife. 2018 Oct 12;7:e38279. doi: 10.755

   Insert

   smad1 (SMAD1 Human)
   

   Use

   Tags

   RFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. Bxb1-GA_AIO-GZV-SynZiFTR_mGL donor

   Plasmid

   #229788

   Purpose

   Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one grazoprevir-inducible mGreenLantern expression

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   mGreenLantern; ZF10-NS3-p65
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   ZF10 and CAG

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSLQ6332 - ACTB CRISPRa CLIP Donor

    Plasmid

    #210015

    Purpose

    CLIP donor for integrating CRISPRa (dCas12-miniVPR)-T2A-GFP-P2A to the ACTB locus

    Depositor

    Stanley Qi

    Article

    Chavez et al Nat Biomed Eng. 2023 May 1. doi: 10.1038

    Insert

    dLbCpf1-miniVPR-T2A-GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDonor-tBFP-NLS-Neo (Universal)

    Plasmid

    #80767

    Purpose

    Universal donor vector for CRISPR/Cas9-mediated homology-independent knock-in system.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

    Insert

    PITCh-gRNA#3 targeting sequence (GCATCGTACGCGTACGTGTT)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. R26-EGFP HMEJ donor vector

    Plasmid

    #137927

    Purpose

    ROSA26 knock-in vector with homology-mediated end joining(HMEJ)-based method

    Depositor

    Hiroshi Kiyonari

    Article

    Abe et al Cell Rep. 2020 May 19;31(7):107653. doi:

    Insert

    SA-EGFP-bpA with homology arms
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. R26-H2B-EGFP HR donor vector

    Plasmid

    #137925

    Purpose

    R0SA26 knock-in vector with homologous recombination-based method

    Depositor

    Hiroshi Kiyonari

    Article

    Abe et al Cell Rep. 2020 May 19;31(7):107653. doi:

    Insert

    SA-H2B-EGFP-bpA with homology arms
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. TRE-TIGHT-EGFP-backward donor

    Plasmid

    #22077

    Depositor

    Rudolf Jaenisch

    Article

    Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

    Insert

    TETO-eGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEF1a-donor_opt(R2Tg)-GFP(intron)

    Plasmid

    #223252

    Purpose

    Mammalian expression of the optimized R2Tg RNA donor

    Depositor

    Wei Li

    Article

    Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

    Insert

    R2Tg donor_opt
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. eGFPbait-E2A-KalTA4-pA donor vector

    Plasmid

    #61069

    Purpose

    for CRISPR/Cas9 mediated insertion of E2A-KalTA4; to be used in combination with a eGFP specific sgRNA (e.g. Plasmid 61051)

    Depositor

    Filippo Del Bene

    Article

    Auer et al Genome Res. 2014 Jan;24(1):142-53. doi:

    Inserts

    eGFPbait
    KalTA4
    

    Use

    Zebrafish expression

    Tags

    E2A

    Expression

    Mutation

    Promoter

    Available since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. R26-H2B-mCherry HR donor vector

    Plasmid

    #137928

    Purpose

    R0SA26 knock-in vector with homologous recombination-based method

    Depositor

    Hiroshi Kiyonari

    Article

    Abe et al Cell Rep. 2020 May 19;31(7):107653. doi:

    Insert

    SA-H2B-mCherry-bpA with homology arms
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLX-N-CTCF-mAID-Halo-donor

    Plasmid

    #139747

    Purpose

    Mouse N-terminal CTCF CRISPR knock-in donor vector with miniAID degron and HaloTag

    Depositor

    Robert Tjian

    Article

    Xie et al Nat Methods. 2020 Apr;17(4):430-436. doi

    Insert

    CTCF CRISPR knock-in (Ctcf Mouse)
    

    Use

    CRISPR

    Tags

    mAID-HaloTag

    Expression

    Mutation

    Promoter

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. peSpCas9(1.1)-2×sgRNA (IFT88, donor)

    Plasmid

    #80769

    Purpose

    Expresses eSpCas9(1.1) and two sgRNAs. The first gRNA targets human IFT88 and the second gRNA targets a pDonor-tBFP-NLS-Neo (Universal).

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

    Insert

    IFT88 gRNA#1 (IFT88 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Fgfr2-T2A-H2B-Halo donor

    Plasmid

    #113117

    Purpose

    Donor plasmid for knock-in T2A-H2B-Halo to the c-terminal of mouse Fgfr2 coding sequence

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    Fgfr2 donor region including homology arms and T2A-H2B-Halo
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge